Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,406,053 C→G 7.4% A185A (GCG→GCC)  PP_4750 ← amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,406,0530CG 7.4% 209.2 / 6.8 68A185A (GCG→GCC) PP_4750amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (27/36);  new base G (2/3);  total (29/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

ACAGCACAAAGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTGGGAATGGCGA  >  NC_002947/5405906‑5406198
                                                                                                                                                   |                                                                                                                                                 
aCAGCACAAAGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCAccgccg                                                                                                                                                  >  4:122502/1‑149 (MQ=255)
 cAGCACAAAGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCAccgccgc                                                                                                                                                 <  3:212640/149‑1 (MQ=255)
  aGCACAAAGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTCCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCAccgccgcc                                                                                                                                                >  5:235591/1‑149 (MQ=255)
    cacaAAGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACcgccgccgc                                                                                                                                              >  3:132568/1‑149 (MQ=255)
     acaAAGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCcgcg                                                                                                                                             <  5:197527/149‑1 (MQ=255)
     acaAAGCACTTCCACAACTCCGGCAACCAAAGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCCACAGCCAACGAGAGAACAAGGCAACTGGCACGCTGAGCACCAGGTACACCAccgcagccgag                                                                                                                                             >  6:82152/1‑147 (MQ=255)
       aaaGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGgt                                                                                                                                           >  8:209062/1‑149 (MQ=255)
           cccTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCGGCGGCGgtggtg                                                                                                                                       <  2:84274/149‑4 (MQ=255)
           cccTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAg                                                                                                                                       <  7:78329/149‑1 (MQ=255)
            ccTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGa                                                                                                                                      >  2:56393/1‑149 (MQ=255)
            ccTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGa                                                                                                                                      >  6:27508/1‑149 (MQ=255)
             cTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGgt                                                                                                                                           <  4:3570/143‑1 (MQ=255)
             cTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGgt                                                                                                                                           >  3:3570/1‑143 (MQ=255)
                ccACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGc                                                                                                                                   <  3:108674/148‑1 (MQ=255)
                      cTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCGGCGGCGgtggtgtacc                                                                                                                                   <  6:126917/142‑8 (MQ=255)
                      cTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCGGCGGCGgtggtgtacc                                                                                                                                   >  5:126917/1‑135 (MQ=255)
                           gCAACGATTTCATGCACTTCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGAAAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTa                                                                                                                                        <  4:230154/132‑1 (MQ=255)
                            cAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTCCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGaaa                                                                                                                      <  6:235591/149‑1 (MQ=255)
                                      aTCCACATCTTCAGCAGTTCTCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACGAGGTACACCACCGACGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCtgt                                                                                                            <  5:82152/149‑1 (MQ=255)
                                         cacaTCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGa                                                                                                          >  2:166475/1‑148 (MQ=255)
                                         cacaTCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGa                                                                                                          >  6:126673/1‑148 (MQ=255)
                                               gTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTg                                                                                                    <  7:184138/148‑1 (MQ=255)
                                                          gCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGaaa                                                                                        <  1:166475/149‑1 (MQ=255)
                                                            tCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGc                                                                                      >  5:239419/1‑149 (MQ=255)
                                                              aTCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGc                                                                                      <  4:29012/147‑1 (MQ=255)
                                                              aTCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGc                                                                                      <  8:35736/147‑1 (MQ=255)
                                                                 gggCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGAt                                                                                  <  6:210993/148‑1 (MQ=255)
                                                                    cAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCTTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCa                                                                              >  6:200175/1‑149 (MQ=255)
                                                                       tCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTATGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCAcc                                                                            >  8:123770/1‑148 (MQ=255)
                                                                          tctGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGAt                                                                                  >  8:109517/1‑139 (MQ=255)
                                                                          tctGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGAt                                                                                  <  7:109517/139‑1 (MQ=255)
                                                                                   aCGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAAtcgtc                                                               >  5:189189/1‑149 (MQ=255)
                                                                                       ggCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAgg                                                           >  8:247130/1‑149 (MQ=255)
                                                                                          cGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCtgtg                                                                                                           <  4:113929/98‑1 (MQ=255)
                                                                                          cGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCtgtg                                                                                                           >  3:113929/1‑98 (MQ=255)
                                                                                                 cAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGcc                                                   >  1:123319/1‑147 (MQ=255)
                                                                                                  aaCGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAg                                                >  2:58188/1‑149 (MQ=255)
                                                                                                   aCGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAAc                                                                         >  7:190622/1‑123 (MQ=255)
                                                                                                   aCGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAAc                                                                         <  8:190622/123‑1 (MQ=255)
                                                                                                     gagagaACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTcc                                             >  5:116686/1‑149 (MQ=255)
                                                                                                       gagaACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCtt                                           <  7:209062/149‑1 (MQ=255)
                                                                                                       gagaACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCtt                                           <  6:239419/149‑1 (MQ=255)
                                                                                                       gagaACAAGGCCACTGGCACGCTGAGCAACTGGTACACCACCGCCGCAGCTGTGTAGACGCTCAGCCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGACTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCt                                            >  1:22231/1‑148 (MQ=255)
                                                                                                        agaACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCTGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTg                                          <  1:180495/149‑1 (MQ=255)
                                                                                                        agaACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCtt                                           <  7:123770/148‑1 (MQ=255)
                                                                                                          aaCAAGGCCTCTGGCACGCTGAGCACCAGGTAGACGACGGCCGCCGCGGTGTAGACTCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAATGGTTAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAg                                        <  2:22231/149‑1 (MQ=255)
                                                                                                           aCAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCTTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGc                                       <  5:200175/149‑1 (MQ=255)
                                                                                                           aCAACGCCCCTGGCACGCCGAGCACCAGGTACCCCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGc                                       <  4:166750/149‑1 (MQ=255)
                                                                                                            cAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCa                                      <  1:58188/149‑1 (MQ=255)
                                                                                                             aaGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAg                                     >  1:89/1‑149 (MQ=255)
                                                                                                              aGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAgg                                    <  5:126673/149‑1 (MQ=255)
                                                                                                               ggCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGc                                   >  5:71091/1‑149 (MQ=255)
                                                                                                                   aCTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAAt                               >  4:27803/1‑149 (MQ=255)
                                                                                                                    cTGGCACGCTGAGCACCAGGTACACCACCGCCTCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATg                              <  6:189189/149‑1 (MQ=255)
                                                                                                                       gCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCt                                                                                     >  4:239125/1‑91 (MQ=255)
                                                                                                                        cACCCTCAGCACCACGAACACCACCGCCGCTGCGTTGTAGACGCTCAGGCCCTGAAAGGATTGCTGTGAAACCTGGTCAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGt                          <  8:47430/149‑1 (MQ=255)
                                                                                                                            cTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGt                      <  5:27508/149‑1 (MQ=255)
                                                                                                                                 cACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCaa                                                                    <  8:130228/98‑1 (MQ=255)
                                                                                                                                 cACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCaa                                                                    >  7:130228/1‑98 (MQ=255)
                                                                                                                                 cACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCt                  >  6:40016/1‑148 (MQ=255)
                                                                                                                                 cACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTg                 <  1:56393/149‑1 (MQ=255)
                                                                                                                                 cACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTg                 >  3:195170/1‑149 (MQ=255)
                                                                                                                                   ccAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCa                                      >  7:140731/1‑126 (MQ=255)
                                                                                                                                   ccAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCa                                      <  8:140731/126‑1 (MQ=255)
                                                                                                                                       gTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTgg           <  3:122502/149‑1 (MQ=255)
                                                                                                                                       gTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTgg           <  3:27803/149‑1 (MQ=255)
                                                                                                                                       cgtctacaccGCGGCGGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTgg           >  8:85332/7‑149 (MQ=255)
                                                                                                                                       cgtctacaccGCGGCGGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTgg           <  7:85332/143‑1 (MQ=255)
                                                                                                                                           accaccGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTGGGAAt       <  4:132568/149‑1 (MQ=255)
                                                                                                                                             caccGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGa                                                 <  3:227657/105‑1 (MQ=255)
                                                                                                                                             caccGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGa                                                 >  4:227657/1‑105 (MQ=255)
                                                                                                                                             caccGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTGGGAATgg     <  7:247130/149‑1 (MQ=255)
                                                                                                                                              accGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTGGGAATGGc    <  5:40016/149‑1 (MQ=255)
                                                                                                                                               ccgccgccgCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTGGGAATGGCg   <  2:123319/149‑1 (MQ=255)
                                                                                                                                                cgccgccgcGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTGGGAATGGCGa  <  6:116686/149‑1 (MQ=255)
                                                                                                                                                 gccgccgcGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCg                           >  6:80817/1‑123 (MQ=255)
                                                                                                                                                 gccgccgcGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCg                           <  5:80817/123‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACAGCACAAAGCCCTTCCACAACTCCGGCAACCATTGCATCCACATCGTCAGCAGTTCGCTCATCGGGCAATCCTCTGTTTCAACGTGGCCGACAGCCAACGAGAGAACAAGGCCACTGGCACGCTGAGCACCAGGTACACCACCGCCGCCGCGGTGTAGACGCTCAGGCCCTGAAAGGTTTGCTGTGAAACCTGGTAAGCCTGAAAGCTGATTTCACCAACGCCAATCGTCGAGGCCACGGCCGAGTCCTTGAGCAGGCCAATGGCGTAGGTGGCTGAGGTGGGAATGGCGA  >  NC_002947/5405906‑5406198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑