| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,518,734 | T→A | 16.8% | K59N (AAA→AAT) ‡ | PP_4851 ← | phosphate starvation‑inducible protein PsiF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,518,734 | 0 | T | A | 16.8% | 102.7 / 16.9 | 54 | K59N (AAA→AAT) ‡ | PP_4851 | phosphate starvation‑inducible protein PsiF |
| Reads supporting (aligned to +/- strand): ref base T (21/23); new base A (5/4); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.76e-01 | |||||||||||
CAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACACCGCCAGCGCCAGTAACGGGATTTGCAGCACTTTCATGGAG > NC_002947/5518586‑5518881 | cAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑t < 5:9942/149‑1 (MQ=255) cttcatcAGGCAATCGCTCATGAAGGCCTTGAGCTCATCCACCTTCAGGGGGTTGGTGGTGGCGTCAGCATTGCAGGTCTTAATTTTTTCATGCTGGGGTTGGGGCGCATCAATTAGGAGGCAGGTGCACATGCCCGA‑GATGCGTTCaa > 8:113879/1‑148 (MQ=255) ttcttcAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATc > 4:110504/1‑149 (MQ=255) cttcAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑AAAGCGTTCAtga < 2:164324/148‑3 (MQ=255) cttcAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑AAAGCGTTCAtg < 7:237028/147‑2 (MQ=255) aGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCtt < 3:163214/149‑1 (MQ=255) gCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑AAAGCGTTc < 2:65478/138‑1 (MQ=255) gCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑AAAGCGTTc > 1:65478/1‑138 (MQ=255) cTCATGAAGGCCTTGCGCTCATCCGCCTTCAGGGATTTGGTGGTGGCGTCAGCATTTCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCtt < 3:197794/149‑1 (MQ=255) cTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTGTTGCTGGGTTTGGGGGACATCCTGTTTCATGACGGTGCTCATGATCGC‑TTTTCGTTCATCGCCCTTGAGGGccgg > 5:152957/1‑147 (MQ=255) aTGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTg < 3:110504/147‑1 (MQ=255) aaGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGt < 3:168524/149‑1 (MQ=255) ccTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAgg > 4:40788/1‑134 (MQ=255) ccTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAgg < 3:40788/134‑1 (MQ=255) cTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGc < 8:213605/147‑1 (MQ=255) cTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGc > 7:213605/1‑147 (MQ=255) cccTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGGACATCCTTTTTCAGGAAGGTGCTCATGAACGC‑TTTGCGTTCATCGACCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGt > 1:155790/1‑149 (MQ=255) cTTCAGGGCTTTGGTTGTTGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTCGGGCACATCGTTTTTCAGGCTGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGttt < 3:196501/149‑1 (MQ=255) tggCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCtgctgc < 1:115079/149‑1 (MQ=255) gCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTCAGGGCCTTGGCGGTGGCGTCGGCCTTGCAGGTTTTCATCTTTTCCTCCTgcgc > 7:250050/1‑149 (MQ=255) gTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCgg > 8:80514/1‑149 (MQ=255) cATTGCAGGTCTTCATATTTTCTTGCTGGGTTTCGGGCACATCCTTTTTCAGGCAGGTCCTCATGACCGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGCTTTTCATCTTTTCCTGCTGCGCGGTATTg > 2:53513/1‑149 (MQ=255) gCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTAATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAg > 5:73858/1‑149 (MQ=255) gTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGa > 8:89647/1‑148 (MQ=255) ttCATTTTTTCTTGCTTTGTTTGGTGCACATCCTTTTTCAGGCATGTGCTCATGAACGC‑TTTGCTTTCATCGCCCTTGAGGGCCTTGGCGGTGTCGTCTGCGTTGCAGTTTTTCATCTTTTCctgct < 5:176950/127‑1 (MQ=255) ttCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGccc < 7:179037/75‑1 (MQ=255) ttCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGccc > 8:179037/1‑75 (MQ=255) ttCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAAcc > 5:78073/1‑149 (MQ=255) ttCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAAAGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTAatgct > 6:176950/1‑127 (MQ=255) aTTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTg > 6:174595/1‑149 (MQ=255) aTTTTTTCATGCTGGGTTTCGGGCACATCCTTTTTCAGGCACGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCtt < 5:51940/148‑1 (MQ=255) ttttttCTTGCTGGGTTTGGTGCACATTCTTTTTCAGGCATGTGCTCATGATCGG‑TTTTCGTTCATAGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTg < 2:155790/148‑1 (MQ=255) ttttttCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTgg < 6:78073/149‑1 (MQ=255) ttttttCTTGCTGGGGGTGGGGCACATCCTTTTTCAGGCAGGGGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTgg < 3:175932/149‑1 (MQ=255) ttttCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTggg < 2:130137/148‑1 (MQ=255) gCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGCGTTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGa > 1:59761/1‑148 (MQ=255) gCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTg < 5:174595/146‑1 (MQ=255) ggTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTgg > 5:98632/1‑136 (MQ=255) ggTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTgg < 6:98632/136‑1 (MQ=255) aTCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACACcgccagcgcca < 2:59761/149‑1 (MQ=255) tttttCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAAACTTGGGCACTGAACAACACCGCCAGCGCCAGTaa > 8:39415/1‑149 (MQ=255) ttCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACACCGCCAGCGCCAGTAAct < 7:80514/148‑2 (MQ=255) aggcaggTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACAcc > 2:109370/1‑129 (MQ=255) aggcaggTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACAcc < 1:109370/129‑1 (MQ=255) aggTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCtt < 5:93453/108‑1 (MQ=255) aggTGCTCATGAAAGC‑TTTGCGTTCATCGCACTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCtt > 6:93453/1‑108 (MQ=255) cgaTGAACGC‑AAAGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCAc > 2:74755/3‑108 (MQ=255) cgaTGAACGC‑AAAGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCAc < 1:74755/106‑1 (MQ=255) gaTGAACGC‑AAAGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACACCGCCAGCGCCAGTAACGGGATTTGCAGCACtt > 5:8691/2‑149 (MQ=255) gaTGAACGC‑AAAGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACACCGCCAGCGCCAGTAACGGGATTTGCAGCACtt > 5:4865/2‑149 (MQ=255) gaTGAACGC‑AAAGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACACCGCCAGCGCCAGTAACGGGATTTGCAGCACtt > 3:75773/2‑149 (MQ=255) gAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGTGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACACCGCCAGCGCCAGTAACGGGATTTGCAGCACTTTCa > 5:108749/1‑149 (MQ=255) gAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACACCGCCAGCGCCAGTAACGGGATTTGCAGCACTTTCa > 7:150433/1‑149 (MQ=255) aaCGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACACCGCCAGCGCCAGTAACGGGATTTGCAGCACTTTCAt < 3:223104/149‑1 (MQ=255) c‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGTGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACACCGCCAGCGCCAGTAACGGGATTTGCAGCACTTTCATGGAg < 6:108749/149‑1 (MQ=255) | CAGGTCATTTCTTCTTCAGGCAATCGCTCATGAAGGCCTTGCGCTCATCCCCCTTCAGGGCTTTGGTGGTGGCGTCAGCATTGCAGGTCTTCATTTTTTCTTGCTGGGTTTGGGGCACATCCTTTTTCAGGCAGGTGCTCATGAACGC‑TTTGCGTTCATCGCCCTTGAGGGCCTTGGCGGTGGCGTCGGCGTTGCAGGTTTTCATCTTTTCCTGCTGCGCGGTATTGGCAGCGAAACCTTGGGCACTGAACAACACCGCCAGCGCCAGTAACGGGATTTGCAGCACTTTCATGGAG > NC_002947/5518586‑5518881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |