Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,552,915 C→A 15.0% intergenic (+578/+83) PP_4882 → / ← PP_5717 iron ABC transporter permease/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,552,9150CA15.0% 72.9 / 7.0 39intergenic (+578/+83)PP_4882/PP_5717iron ABC transporter permease/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (12/21);  new base A (4/2);  total (16/23)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.10e-01

GGTACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTGTA  >  NC_002947/5552767‑5553059
                                                                                                                                                    |                                                                                                                                                  
ggTACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTc                                                                                                                                                    <  3:158525/149‑1 (MQ=255)
  tACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGc                                                                                                                                                  <  5:233710/149‑1 (MQ=255)
     tCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCa                                                                                                                                                 >  7:189934/1‑147 (MQ=255)
       aTTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCaaa                                                                                                                                               <  1:266086/147‑1 (MQ=255)
       aTTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACAGGAAGGTCCGGGCTTTTGTTAGCaaatc                                                                                                                                             >  7:118302/1‑147 (MQ=18)
       aTTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTCAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGACCGCGCATTGCTTCGCAAAga                                                                                                                                             >  4:163066/1‑148 (MQ=255)
          ttCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGCTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGAAGCAAAG‑ccc                                                                                                                                          <  1:198507/148‑1 (MQ=255)
             gcgcTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGa                                                                                                                                       <  2:19072/148‑1 (MQ=255)
               gcTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAAc                                                                                                                                    >  2:55902/1‑149 (MQ=255)
                 tctcATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGAAGCAAAG‑cc                                                                                                                                           >  5:207908/1‑140 (MQ=255)
                 tctcATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGAAGCAAAG‑cc                                                                                                                                           <  6:207908/140‑1 (MQ=255)
                                      aGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAGCCCCGGAAACTGGGGTTTCAGCTGGTGgctg                                                                                                               <  1:20720/148‑1 (MQ=255)
                                           tgcttcttGGTGAACCGGCTGTACACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCAGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAgt                                                                                                        >  3:87260/8‑149 (MQ=17)
                                             aCGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAgtgt                                                                                                      >  6:67386/1‑149 (MQ=255)
                                               gTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGATGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGttt                                                                                                    <  8:100318/149‑1 (MQ=255)
                                                       tACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGACCCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGGTTCGCCAAG‑CCCGGCAACTGGGGTTTCAGGTGGTGGCTGGTTGAGTGTTTAGAAAAc                                                                                             >  8:55667/1‑148 (MQ=17)
                                                       tACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGaaaa                                                                                              <  7:36350/147‑1 (MQ=255)
                                                          aGCCGGTTCACCAAGAAGCAGGCTTATGCTGATCAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTTCTTCTCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATg                                                                                         <  3:163066/149‑1 (MQ=255)
                                                            ccGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑GCCGGAAACTGGGGGTTAAGCAGGTGGCTGGTGTAGTGGTTAGATAAGAATtg‑g                                                                                      >  8:108010/1‑149 (MQ=17)
                                                                tctaCCAATAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTg                                                                                   <  5:164293/145‑1 (MQ=255)
                                                                   aCCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCAtt                                                                               <  7:220438/149‑1 (MQ=255)
                                                                     cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCt                                                                             >  3:216900/1‑149 (MQ=255)
                                                                              ggCTTATGCTGAACATGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACGGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCa                                                                    <  3:109730/149‑1 (MQ=255)
                                                                               gCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGttt                                                                         <  8:170287/143‑1 (MQ=255)
                                                                               gCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGttt                                                                         >  7:170287/1‑143 (MQ=255)
                                                                                cTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAgg                                                                  <  4:216900/149‑1 (MQ=255)
                                                                                   aTGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGc                                                                <  5:67386/148‑1 (MQ=255)
                                                                                         aaCAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCATGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGGTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGCTTAc                                                            <  5:9710/146‑1 (MQ=255)
                                                                                           cAAGTTCTTGAGTAATTCCTAACCATAATCCAATACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAgg                                                                  <  8:168480/138‑1 (MQ=17)
                                                                                                      ggAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATc                                             <  1:214496/148‑1 (MQ=255)
                                                                                                         aTTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTTCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTTGTGGCTGCTTGAGTGTTTAGAAAACAATGGCGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCaa                                          <  1:55902/149‑1 (MQ=255)
                                                                                                          ttCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCa                                           >  5:257154/1‑146 (MQ=255)
                                                                                                             cGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCAGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCA‑GGCTTACCTCAAGTACTCTATCCAAAGaaaa                                    <  4:87260/149‑1 (MQ=255)
                                                                                                               aaCCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGaaaaa                                   <  6:257154/149‑1 (MQ=255)
                                                                                                                  cAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCGTGAAACTGGGGTTTCAGCTGGTGTCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGaaaaaaaa                                <  5:34418/149‑1 (MQ=255)
                                                                                                                        ccAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACaa                          >  1:200938/1‑149 (MQ=255)
                                                                                                                                        cGGGCTTTGCGAAGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCa           >  4:91511/1‑148 (MQ=255)
                                                                                                                                         gggCTTTGCGAAGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAg             >  1:267784/1‑145 (MQ=255)
                                                                                                                                                 cTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTGTa  <  1:174805/148‑1 (MQ=255)
                                                                                                                                                  ttCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAAACAGCAAAATATGTa  >  6:265504/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGTACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGG‑GTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTGTA  >  NC_002947/5552767‑5553059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑