Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,570,178 G→C 9.5% A24G (GCA→GGA) ‡ nnrD ← ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,570,1780GC 9.5% 125.6 / 4.9 42A24G (GCA→GGA) ‡nnrDADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (18/20);  new base C (2/2);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCCTC  >  NC_002947/5570032‑5570324
                                                                                                                                                  |                                                                                                                                                  
gCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCtgccgtgcc                                                                                                                                                  >  3:147784/1‑149 (MQ=255)
 cTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCtgccgtgccg                                                                                                                                                 <  7:30435/149‑1 (MQ=255)
 cTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCtgccgtgccg                                                                                                                                                 >  2:102511/1‑149 (MQ=255)
    gggCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCgg                                                                                                                                                >  2:250350/1‑147 (MQ=255)
    gggCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCgg                                                                                                                                                <  2:242759/147‑1 (MQ=255)
         gggTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCgg                                                                                                                                         >  7:230583/1‑149 (MQ=255)
           ggtGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGGCACGGCAGGCgcat                                                                                                                                       <  8:197483/147‑4 (MQ=255)
            ttGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCACCTGCCGTGCCGGCAGGCGGGc                                                                                                                                       >  8:36165/1‑148 (MQ=255)
               ccACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCCCCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAAcc                                                                                                                                   >  6:58839/1‑149 (MQ=255)
               ccACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGGCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAAcc                                                                                                                                   >  5:235147/1‑149 (MQ=255)
                 actcTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGt                                                                                                                                 <  8:230583/146‑1 (MQ=255)
                   gCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCa                                                                                                                               <  1:261319/149‑1 (MQ=255)
                    cTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCAc                                                                                                                              <  7:215716/149‑1 (MQ=255)
                          aGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACgctgct                                                                                                                        <  5:110158/149‑1 (MQ=255)
                               ggCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGc                                                                                                                    >  6:73665/1‑148 (MQ=255)
                               ggCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGc                                                                                                                    >  5:69054/1‑148 (MQ=255)
                                  gccgcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAAtt                                                                                                                <  1:250350/149‑1 (MQ=255)
                                     gcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCGGGCGGGCAACCGTCACGCTGCTGAGCAATtgtg                                                                                                             <  4:209419/149‑1 (MQ=255)
                                       aaCGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCTCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg                                                                                                           <  7:36165/149‑1 (MQ=255)
                                         cGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGc                                                                                                         <  6:69054/149‑1 (MQ=255)
                                                   cTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTgggg                                                                                               >  3:201459/1‑149 (MQ=255)
                                                         cACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTg                                                                                         <  4:147784/149‑1 (MQ=255)
                                                         cACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGGCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTg                                                                                         <  5:55402/149‑1 (MQ=255)
                                                                  acacAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCgg                                                                                 >  3:66119/1‑148 (MQ=255)
                                                                           cTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCAGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTc                                                                        >  7:75951/1‑148 (MQ=255)
                                                                                   cccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTc                                                                <  4:66119/148‑1 (MQ=255)
                                                                                             ccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                      >  5:179628/1‑148 (MQ=255)
                                                                                               ccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                      >  2:211693/1‑146 (MQ=255)
                                                                                                     accaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtat                                             <  5:73665/149‑1 (MQ=255)
                                                                                                     accaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtat                                             <  1:211693/149‑1 (MQ=255)
                                                                                                      ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg                                                                                                           >  5:45647/1‑86 (MQ=255)
                                                                                                      ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg                                                                                                           <  6:45647/86‑1 (MQ=255)
                                                                                                       caGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATAc                                           <  5:58839/149‑1 (MQ=255)
                                                                                                         gTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTAGCAGAATTATACGc                                         <  3:148485/149‑1 (MQ=255)
                                                                                                                gCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAg                                  >  7:13327/1‑149 (MQ=255)
                                                                                                                 ccAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGc                                 <  8:13327/149‑1 (MQ=255)
                                                                                                                       tCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAt                           >  6:180955/1‑149 (MQ=255)
                                                                                                                       tCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAt                           >  6:176999/1‑149 (MQ=255)
                                                                                                                        cGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCTGTAtt                          <  8:238970/149‑1 (MQ=255)
                                                                                                                         gCCTTTGTGGGCATGCGCCTGCCGTGTCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttg                         >  2:122990/1‑149 (MQ=255)
                                                                                                                                  ggCATGCGCCTGCCGTGCCGGCAGGCGGGTAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGc                                                     <  2:52925/112‑1 (MQ=255)
                                                                                                                                  ggCATGCGCCTGCCGTGCCGGCAGGCGGGGAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGc                                                     >  1:52925/1‑112 (MQ=255)
                                                                                                                                         gcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                      <  7:89435/104‑1 (MQ=255)
                                                                                                                                         gcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                      >  8:89435/1‑104 (MQ=255)
                                                                                                                                          ccTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTc        >  2:257424/1‑149 (MQ=255)
                                                                                                                                              ccgtgccgGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGGCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGcc    <  1:102511/149‑1 (MQ=255)
                                                                                                                                                gtgccgGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCctc  >  4:36929/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCCTC  >  NC_002947/5570032‑5570324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑