Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,611,354 C→A 5.3% F116L (TTC→TTA)  PP_4931 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,611,3540CA 5.3% 254.9 / 3.2 76F116L (TTC→TTA) PP_4931hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (38/34);  new base A (2/2);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GGAAGCCATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTCCCGAGCCTGCTGGCCAACC  >  NC_002947/5611210‑5611493
                                                                                                                                                |                                                                                                                                           
ggAAGCCATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGc                                                                                                                                           <  8:183214/147‑1 (MQ=255)
ggAAGCCATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCa                                                                                                                                         <  3:69041/149‑1 (MQ=255)
ggAAGCCATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCa                                                                                                                                         <  5:186077/149‑1 (MQ=255)
     ccATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAg                                                                                                                                    <  5:66302/149‑1 (MQ=255)
      cATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGTCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGCTGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCACCCTCACAAGCTTCTTAGCCAGCAAgg                                                                                                                                   >  8:57331/1‑149 (MQ=255)
      cATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGTCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTAGCCAGCAAgg                                                                                                                                   >  8:60983/1‑149 (MQ=255)
       aTCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGc                                                                                                                                  >  7:137280/1‑149 (MQ=255)
          gCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGt                                                                                                                               >  4:27840/1‑149 (MQ=255)
                     gcgccgcTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTg                                                                                                                              >  5:51083/1‑139 (MQ=255)
                     gcgccgcTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTg                                                                                                                              <  6:51083/139‑1 (MQ=255)
                          gcTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGAc                                                                                                               <  5:54826/149‑1 (MQ=255)
                          gcTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGAc                                                                                                               <  2:208219/149‑1 (MQ=255)
                                gcgAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGt                                                                                                          >  4:98040/1‑148 (MQ=255)
                                   aGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAg                                                                                                      >  5:63851/1‑149 (MQ=255)
                                    ggCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGTGCGGCCGGGTCGACCAGGTCAAgg                                                                                                     <  2:254802/149‑1 (MQ=255)
                                    ggCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAgg                                                                                                     <  6:63851/149‑1 (MQ=255)
                                        ttGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGa                                                                                                 >  2:137591/1‑149 (MQ=255)
                                          ggCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGAc                                                                                                <  3:98040/148‑1 (MQ=255)
                                          ggCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGAc                                                                                                <  3:195292/148‑1 (MQ=255)
                                              ggCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGtt                                                                                           <  5:212143/149‑1 (MQ=255)
                                                 aaCGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGc                                                                                         >  3:136079/1‑148 (MQ=255)
                                                     gCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCg                                                                                    <  2:248655/149‑1 (MQ=255)
                                                             gACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCAACGGCACTg                                                                              <  5:265437/147‑1 (MQ=255)
                                                               ccTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAgg                                                                                                     <  1:131110/122‑1 (MQ=255)
                                                               ccTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAgg                                                                                                     >  2:131110/1‑122 (MQ=255)
                                                                cTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGa                                                                         >  5:174940/1‑149 (MQ=255)
                                                                   aGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACcg                                                                      >  3:83527/1‑149 (MQ=255)
                                                                    gTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACcgc                                                                     <  8:137280/149‑1 (MQ=255)
                                                                          cccGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGtt                                                                >  6:127058/1‑148 (MQ=255)
                                                                           ccGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTc                                                               >  4:73107/1‑148 (MQ=255)
                                                                             gCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAg                                                            >  5:61214/1‑149 (MQ=255)
                                                                                 gCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGc                                                         >  4:210414/1‑148 (MQ=255)
                                                                                   tctcCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAg                                                      >  3:54297/1‑149 (MQ=255)
                                                                                     tcCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAg                                                      <  3:210414/147‑1 (MQ=255)
                                                                                     tcCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAg                                                      <  3:27840/147‑1 (MQ=255)
                                                                                     tcCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGgt                                                    >  7:72218/1‑149 (MQ=255)
                                                                                       cGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGgtgt                                                  <  6:174940/149‑1 (MQ=255)
                                                                                       cGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGgtg                                                   >  5:52525/1‑148 (MQ=255)
                                                                                         aTGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTc                                                                                                                  <  6:252933/83‑1 (MQ=255)
                                                                                         aTGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTc                                                                                                                  >  5:252933/1‑83 (MQ=255)
                                                                                             ccACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGgt                                                    <  4:119201/141‑1 (MQ=255)
                                                                                             ccACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGgt                                                    >  3:119201/1‑141 (MQ=255)
                                                                                             ccACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTAGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGcc                                            <  7:57331/149‑1 (MQ=255)
                                                                                             ccACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTAGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGcc                                            <  7:60983/149‑1 (MQ=255)
                                                                                              cACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCt                                           <  5:127058/149‑1 (MQ=255)
                                                                                                   ccTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGgt                                                    <  4:15558/135‑1 (MQ=255)
                                                                                                   ccTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGgt                                                    >  3:15558/1‑135 (MQ=255)
                                                                                                   ccTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGc                                        <  4:83527/147‑1 (MQ=255)
                                                                                                    cTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCgg                                      >  2:23665/1‑148 (MQ=255)
                                                                                                         gTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTg                                          <  5:59568/139‑1 (MQ=255)
                                                                                                         gTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTg                                          >  6:59568/1‑139 (MQ=255)
                                                                                                         gTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGc                                        >  2:53323/1‑141 (MQ=255)
                                                                                                         gTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGc                                        <  1:53323/141‑1 (MQ=255)
                                                                                                         gTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACtgg                                <  1:23665/149‑1 (MQ=255)
                                                                                                           cGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACtggtg                              >  3:76276/1‑149 (MQ=255)
                                                                                                               gggCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCg                          <  1:137591/149‑1 (MQ=255)
                                                                                                                ggCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGa                         >  3:111997/1‑149 (MQ=255)
                                                                                                                 gCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGAt                        <  4:76276/149‑1 (MQ=255)
                                                                                                                 gCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGAt                        >  7:15670/1‑149 (MQ=255)
                                                                                                                     cTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTcc                    >  3:116952/1‑149 (MQ=255)
                                                                                                                     cTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTcc                    >  3:121034/1‑149 (MQ=255)
                                                                                                                        ggCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTccc                   >  1:91818/1‑147 (MQ=255)
                                                                                                                         gCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTCCCGa                 <  6:61214/148‑1 (MQ=255)
                                                                                                                          ccGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTCCCGAGc               >  4:203554/1‑149 (MQ=255)
                                                                                                                           cGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAg                                                      <  4:142262/109‑1 (MQ=255)
                                                                                                                           cGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAg                                                      >  3:142262/1‑109 (MQ=255)
                                                                                                                           cGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTCCCGAGcc              >  4:251344/1‑149 (MQ=255)
                                                                                                                               aaCCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGCTGTCCCGAGCctgct          >  8:132159/1‑149 (MQ=255)
                                                                                                                                      caAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTCCCGAGCCTGCTGGCCAAc   >  3:72432/1‑149 (MQ=255)
                                                                                                                                      caAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTCCCGAGCCTGCTGGCCAAc   <  2:91818/149‑1 (MQ=255)
                                                                                                                                      caAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTCCCGAGCCTGCTGGCCAAc   >  2:75256/1‑149 (MQ=255)
                                                                                                                                       aaGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTg                                          >  2:80177/1‑109 (MQ=255)
                                                                                                                                       aaGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTg                                          <  1:80177/109‑1 (MQ=255)
                                                                                                                                       aaGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTCCCGAGCCTGCTGGCCAAcc  >  6:87805/1‑149 (MQ=255)
                                                                                                                                         gCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTc                     <  3:180777/128‑1 (MQ=255)
                                                                                                                                         gCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTc                     >  4:180777/1‑128 (MQ=255)
                                                                                                                                              ttcGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTCCCGAGCCTGCTGGc       <  7:132223/137‑1 (MQ=255)
                                                                                                                                              ttcGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTCCCGAGCCTGCTGGc       >  8:132223/1‑137 (MQ=255)
                                                                                                                                                |                                                                                                                                           
GGAAGCCATCGCCGACATGCCGCGCCGCTGGCGCGAGGCCTTGGCCGGCAACGGCGAACTCGACCTCAGTCGTACCCGCCTGCTCTCCGATGCCCACTACCTGTGGTCGCCGGGCACCTTGGCCGGCAACCTCACAAGCTTCTTCGCCAGCAAGGCCGTGCGCGGCCGGGTCGACCAGGTCAAGGGTGACCAGTTGCCACCGGCACTGCAGGACCGCGCGTTCAAGGGCAAGGTGTACCGCCTGGCGGAACTGGTGGTCGATGTCCCGAGCCTGCTGGCCAACC  >  NC_002947/5611210‑5611493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑