| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 445,095 | T→C | 14.5% | intergenic (+68/‑220) | PP_0367 → / → PP_0368 | hypothetical protein/acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 445,095 | 0 | T | C | 14.5% | 65.4 / 3.8 | 30 | intergenic (+68/‑220) | PP_0367/PP_0368 | hypothetical protein/acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (10/14); new base C (2/2); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01 | |||||||||||
GACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCT‑ACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACG > NC_002947/444949‑445237 | gACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCa < 1:71259/149‑1 (MQ=255)gACCGAAGTCCACCTAGGCCCAAAGTTATTCAAGCCATCC‑GACGAAATGCTTGTCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCa < 6:235531/149‑1 (MQ=255) aaGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCTGGCTTGCCCGCGAATGCGTCCGATc < 4:73919/143‑1 (MQ=255) aaGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATc > 3:73919/1‑143 (MQ=255) tCGAGCTCGGCGCAAAGGTCGTCAAGGCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGacaaaat > 5:214908/1‑146 (MQ=38) gCTCGGCGCAAAGGTCGTCAAGGCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAAtttt < 5:72009/149‑1 (MQ=255) cTCGGCGCAAAGGTCGTCAAGGCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTg < 1:237921/149‑1 (MQ=255) tCGGCGCAAAGGTCGTCAAGGCATCCAGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTg > 1:140209/1‑149 (MQ=255) ggCGCAAAGGTCGTCAAGGCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGt < 6:112385/147‑1 (MQ=255) ggCGCAAAGGTCGTCAAGGCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCt < 7:249476/149‑1 (MQ=255) gcgcAAAGGTCGTCAAGGCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTg > 3:189963/1‑149 (MQ=255) gCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCgg < 4:189963/149‑1 (MQ=255) gCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCgg < 6:214908/149‑1 (MQ=18) aTCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGc < 2:140209/149‑1 (MQ=255) aggCTTGGCCGGATCATCCACACCTACGCCTTAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTGGTAGGAGCGGGTTTGCCCTCTAATGCGTCCGATCAGACAAAATTTTTGTCTGGTGGGGCGTATTCGGGGGAAAGCCCGCTc < 6:88091/147‑1 (MQ=255) tGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCGGGCAAGccc > 2:58636/1‑140 (MQ=14) tGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCGGGCAAGccc < 1:58636/140‑1 (MQ=14) ccTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCATTCCCGCGAAAGCGTCCGAACAGACAACAAATTTGTCAGGTCGGGCCACTTAGCGGGGAACCCCCCTc > 3:62328/1‑127 (MQ=255) tGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATAAGACAACAATTTTGTCTGGTCGGGCCTCTTAGCGGGCAAGCCCGCTCCT‑ACAAGGGTTTTTGGCATGGGATGACTGa > 1:170622/1‑149 (MQ=255) gAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCt < 2:150464/88‑1 (MQ=255) gAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCt > 1:150464/1‑88 (MQ=255) aaTACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTATGGTGGGGCCTCTTAGCGGGGAAGCCCGCTCCG‑AAAAGGGTTTGTGGCATGGCATTACTGAtt > 8:73320/1‑149 (MQ=39) tGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCCCGAAATGGTCCGAAAACACAAAAAATTTTTATGGGGGGGGCGCTTAGCGGGGAAGCCCCCCACTAACAAGGTTTTTTGG‑ATGTCATGACATATTTTCCAt > 4:72329/1‑149 (MQ=255) ccTATTTCAGCGCCTGCCTTGTTGGCGCGGGCTTGCCCGCTAATGCGTCCGATCCCACAACATTTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCT‑ACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGc < 2:27098/149‑1 (MQ=38) ttGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCt < 4:193116/39‑1 (MQ=255) ttGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCt > 3:193116/1‑39 (MQ=255) cccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGTGCCTCTTCGCGGGCAAGCCCGCTCCT‑ACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGc > 4:215236/1‑149 (MQ=255) cccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCT‑ACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGc > 6:133673/1‑149 (MQ=255) gcgAATGCGTCCTATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCT‑ACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGa < 2:170622/149‑1 (MQ=255) tCCGATCAGACAACAATATTGTCTCGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCT‑ACAAGGGTTTGTGGCATGGCATGACTCATTAGCCAATTTTCGTGGAGTAAATGGCACCATACTCCATCCATGACAGAAAGCTCAGCTAAAca > 4:172979/1‑147 (MQ=255) | GACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCC‑GACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCT‑ACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACG > NC_002947/444949‑445237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |