Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,628,555 T→G 10.0% intergenic (‑25/+82) rlmJ ← / ← putP 23S rRNA (adenine(2030)‑N(6))‑methyltransferase/sodium/L‑proline transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,628,5550TG10.0% 137.7 / 6.2 49intergenic (‑25/+82)rlmJ/putP23S rRNA (adenine(2030)‑N(6))‑methyltransferase/sodium/L‑proline transporter
Reads supporting (aligned to +/- strand):  ref base T (21/23);  new base G (3/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCGATATAGGCGAACGGCTGCTCCTTGCGCGACATCAGGGCGATGAGGCGGGTCAGCACGATGTGTTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCGAAGCGCTTGACCATCGTCTGCGAAGGGCT  >  NC_002947/5628413‑5628702
                                                                                                                                              |                                                                                                                                                   
tATTGATATTGGCGATCGGCTGCTCCTTGAGCGACATCAGGGCGATGAGGCGGGGCAGCACGATGTGTTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTAc                                                                                                                                                 <  2:199812/147‑1 (MQ=255)
tATCGATATAGGCGAACGGCTGCTCCTTGCGCGACATCAGGGCGATGAGGCGGGTCAGCACGATGTGTTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTAcct                                                                                                                                               <  6:91473/149‑1 (MQ=255)
tATCGATATAGGCGAACGGCTGATCCTTGCGCGACATCAGGGCGATGAGGCGGGTCAGCACGATGTGTTTGAGGACGTCGGCTTGGTTGCCGGAGTGGAAGGCGTGACGCTAGTTCATGGCCACTCCTGCGAAGGCGGCAAGtttcc                                                                                                                                                 >  1:199812/1‑145 (MQ=255)
      tataGGCGAACGGCTGCTCGTTGCGCGACATCAGGGCGATGAGGCGTGTCAGCACGATGTGTTTGAGGACGTCGGCGTGGGTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTAccttgcctt                                                                                                                                         <  5:209932/149‑1 (MQ=255)
           gCGAACGGCTGCTCCTTGCGCGACATCAGGGCGATGAGGCGGGTCAGCACGATGTGTTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGGTAAacttgccgc                                                                                                                                         <  1:148254/144‑3 (MQ=255)
           gCGAACGGCTGCTCCTTGCGCGACATCAGGGCGATGAGGCGGGTCAGCACGATGTGTTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGGTAAacttgccgc                                                                                                                                         >  2:148254/1‑142 (MQ=255)
                cGGCTGCTCCTTGCGCGACATCAGGGCGATGAGGCGGGTCAGCACGATGTGTTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAg                                                                                                                               <  1:131715/149‑1 (MQ=255)
                      ctCCTTGCGCGACATCAGGGCGATGAGGCGGGTCAGCACGATGTGTTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAg                                                                                                                           <  4:215043/147‑1 (MQ=255)
                                           gATGAGGCGGGTCAGCACGATGTGTTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCAc                                                                                                    >  7:159615/1‑149 (MQ=255)
                                                ggCGGGTCAGCACGATGTGTTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGGTAAacttgccg                                                                                                                                          >  4:145899/1‑105 (MQ=255)
                                                ggCGGGTCAGCACGATGTGTTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGGTAAacttgccg                                                                                                                                          <  3:145899/106‑2 (MQ=255)
                                                     gTCAGCACGATGTGTTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAg                                                                                            >  2:112414/1‑147 (MQ=255)
                                                                  gtTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCGTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAAt                                                                              >  7:223043/1‑148 (MQ=255)
                                                                      gAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAAc                                                                         >  8:55076/1‑149 (MQ=255)
                                                                      gAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAAc                                                                         >  6:216749/1‑149 (MQ=255)
                                                                       aGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGgct                                                                                                                   <  1:104142/106‑1 (MQ=255)
                                                                       aGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGgct                                                                                                                   >  2:104142/1‑106 (MQ=255)
                                                                          aCGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGgt                                                                     <  3:241973/149‑1 (MQ=255)
                                                                            gTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGgtgt                                                                   >  3:122343/1‑149 (MQ=255)
                                                                            gTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGgtgt                                                                   >  3:66270/1‑149 (MQ=255)
                                                                            gTCGGAGTGGTTGCCGGCGTCGAAGCAGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACATTGCATTGCAGGCGGAGTCAGGCATGGCTGCTGATAGGCGGCACCAGCAAAGCAGGCGCCGCCAATTAAACGGgagt                                                                   >  8:139277/1‑149 (MQ=255)
                                                                               ggCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTggc                                                                                                         >  7:169408/1‑108 (MQ=255)
                                                                               ggCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTggc                                                                                                         <  8:169408/108‑1 (MQ=255)
                                                                               ggCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTAc                                                                <  7:55076/149‑1 (MQ=255)
                                                                               ggCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTACTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTa                                                                 <  5:216749/148‑1 (MQ=255)
                                                                                gCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACt                                                               >  5:191095/1‑149 (MQ=255)
                                                                                      ttGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCCGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCgg                                                         >  3:207615/1‑149 (MQ=255)
                                                                                         ccGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCg                                                       <  8:159615/148‑1 (MQ=255)
                                                                                         ccGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGt                                                      <  1:112414/149‑1 (MQ=255)
                                                                                         ccGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGAAAGGTTACCTTTCCTTGCAGGCCGAGTCAGGCCTGGCATCTTATTTGCCGCACCCCCAAAGCAAGCCCCGCCAATTAAACGGGTGGTAATTGtacgcct                                                      >  1:63532/1‑143 (MQ=255)
                                                                                          cGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCaa                                                                               >  8:210169/1‑123 (MQ=255)
                                                                                          cGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCaa                                                                               <  7:210169/123‑1 (MQ=255)
                                                                                            gCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGt                                                      <  2:175930/146‑1 (MQ=255)
                                                                                            gCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGt                                                      >  1:175930/1‑146 (MQ=255)
                                                                                                     gCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCAt                                          >  8:211330/1‑149 (MQ=255)
                                                                                                            gATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGc                                   >  3:115122/1‑149 (MQ=255)
                                                                                                                 ttCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCg                               >  2:65396/1‑148 (MQ=255)
                                                                                                                  tCATGGCAACTCCTGCGAAGGCGGAAAGTTTACCTTGACTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAATCAGGCGACGCCAATTAAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCg                               >  8:123981/1‑147 (MQ=255)
                                                                                                                   cATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCGaa                             <  4:122343/148‑1 (MQ=255)
                                                                                                                    aTGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCGAAgc                           >  1:271193/1‑149 (MQ=255)
                                                                                                                     tGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCGAAgcg                          <  7:29845/149‑1 (MQ=255)
                                                                                                                              cTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCGaa                             >  6:54008/1‑137 (MQ=255)
                                                                                                                              cTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCGaa                             <  5:54008/137‑1 (MQ=255)
                                                                                                                                gCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGc                                   >  1:54059/1‑129 (MQ=255)
                                                                                                                                gCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGc                                   <  2:54059/129‑1 (MQ=255)
                                                                                                                                   aaGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCGAAGCGCTTGACCATCGTCt            <  2:271193/149‑1 (MQ=255)
                                                                                                                                    aGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCGAAGCGCTTGACCATCGTCTg           <  8:180042/149‑1 (MQ=255)
                                                                                                                                    aGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCGAAGCGCTTGACCATCGTCTg           <  4:115122/149‑1 (MQ=255)
                                                                                                                                           aaGTGTACCTTTCCGTGTAGGCGGAGTCCGGCCTGGCGGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCGAAGCGCTTGACCATCGTCTGCGAAggg    <  8:223043/149‑1 (MQ=255)
                                                                                                                                             gTTTACCTTGCCTTGCAGTCGGAGTCATGGCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCAAATTCAACTGGTGTTACTTGTCGTCGTGATTCGCCGCATCATCCGCTTCGAAGCGCTTGACCATCGTCTGCGAAGGGCt  <  6:146060/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
TATCGATATAGGCGAACGGCTGCTCCTTGCGCGACATCAGGGCGATGAGGCGGGTCAGCACGATGTGTTTGAGGACGTCGGCGTGGTTGCCGGCGTGGAAGGCGTGACGATAGTTCATGGCAACTCCTGCGAAGGCGGCAAGTTTACCTTGCCTTGCAGGCGGAGTCAGGCCTGGCTGCTGATTGGCGGCACCCGCAAAGCAGGCGCCGCCAATTCAACGGGTGTTACTTGTCGGCGTGATACGCCGCATCAGCCGCTTCGAAGCGCTTGACCATCGTCTGCGAAGGGCT  >  NC_002947/5628413‑5628702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑