Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,679,142 G→T 9.8% E256D (GAG→GAT)  bioA → adenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,679,1420GT 9.8% 114.5 / 4.4 41E256D (GAG→GAT) bioAadenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA
Reads supporting (aligned to +/- strand):  ref base G (17/20);  new base T (2/2);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01

GCCGAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTAC  >  NC_002947/5678999‑5679283
                                                                                                                                                 |                                                                                                                                             
gCCGAACACCACGCCTCGTTCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGGCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATc                                                                                                                                            <  1:202498/148‑1 (MQ=255)
   gAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGt                                                                                                                                       >  4:222866/1‑149 (MQ=255)
      caccacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGGCGATCTCGTCGTgga                                                                                                                                    <  7:35130/149‑2 (MQ=255)
        ccacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGCGGCCTGCGCCCGCTATGACGTGCACCTTATC‑CACGCCGAGATCGCCGTGGGCt                                                                                                                                  >  7:173651/1‑149 (MQ=255)
         cacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCtt                                                                                                                                 <  3:222866/149‑1 (MQ=255)
            gCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCgg                                                                                                                              <  8:210182/149‑1 (MQ=255)
                          cGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGTCGCACCGGCACGa                                                                                                                >  7:205484/1‑149 (MQ=255)
                           gTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGAt                                                                                                               >  7:122550/1‑149 (MQ=255)
                                       ccGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGa                                                                                                   <  6:61361/149‑1 (MQ=255)
                                             aTACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAg                                                                                              <  6:173137/148‑1 (MQ=255)
                                                        cGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCATGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGcc                                                                                  >  8:154823/1‑149 (MQ=255)
                                                                tGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTc                                                                          <  8:122550/149‑1 (MQ=255)
                                                                tGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCATGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTc                                                                          <  7:154823/149‑1 (MQ=255)
                                                                    cATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGTCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgt                                                                      <  8:205484/149‑1 (MQ=255)
                                                                    cATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgt                                                                      <  3:153181/149‑1 (MQ=255)
                                                                    cATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgt                                                                      <  2:222988/149‑1 (MQ=255)
                                                                      tGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGc                                                                    <  5:174165/149‑1 (MQ=255)
                                                                                      ccTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCGCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGTCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCt                                                      >  1:199253/1‑148 (MQ=255)
                                                                                       cTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTc                                                                          >  7:101153/1‑126 (MQ=255)
                                                                                       cTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTc                                                                          <  8:101153/126‑1 (MQ=255)
                                                                                        tCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGc                                                         >  3:230017/1‑142 (MQ=255)
                                                                                        tCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGc                                                         <  4:230017/142‑1 (MQ=255)
                                                                                            gttg‑ttGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACcggcg                                              >  7:249526/1‑149 (MQ=255)
                                                                                                  tgCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTAc                                         >  7:92035/1‑149 (MQ=255)
                                                                                                    cgcgAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCt                                       <  7:253834/149‑1 (MQ=255)
                                                                                                      cgAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGTCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGc                                     <  2:199253/149‑1 (MQ=255)
                                                                                                           ccTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTg                                >  6:122276/1‑149 (MQ=255)
                                                                                                            gtGCGACCGCTATGACGTGCAGCTTATC‑CACGTCGAGATCGCCGTGGGCTTCTGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGcc                                                        <  4:122042/123‑1 (MQ=255)
                                                                                                            cTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGcc                                                        >  3:122042/1‑124 (MQ=255)
                                                                                                                          aCGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGac                  >  4:143506/1‑148 (MQ=255)
                                                                                                                             tGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGaccacc              <  8:92035/149‑1 (MQ=255)
                                                                                                                                 ccTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctgc                       <  4:263056/136‑1 (MQ=255)
                                                                                                                                 ccTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctgc                       >  3:263056/1‑136 (MQ=255)
                                                                                                                                  gaagcc‑cACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGAc           >  4:199752/6‑147 (MQ=255)
                                                                                                                                     aTC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgt      >  4:157803/1‑149 (MQ=255)
                                                                                                                                      tC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgt      <  1:54814/148‑1 (MQ=255)
                                                                                                                                      cc‑cACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctgcct                     <  7:243652/132‑1 (MQ=255)
                                                                                                                                      cc‑cACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctgcct                     >  8:243652/2‑133 (MQ=255)
                                                                                                                                            gacgaGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTAc  >  6:203775/1‑147 (MQ=255)
                                                                                                                                              cgaGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgt      >  2:79037/1‑141 (MQ=255)
                                                                                                                                              cgaGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgt      <  1:79037/141‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GCCGAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTG‑TTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTAC  >  NC_002947/5678999‑5679283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑