Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,679,147 C→T 9.5% A258V (GCC→GTC)  bioA → adenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,679,1470CT 9.5% 112.9 / 2.8 42A258V (GCC→GTC) bioAadenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA
Reads supporting (aligned to +/- strand):  ref base C (18/20);  new base T (2/2);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

GAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGTTCTAT  >  NC_002947/5679002‑5679295
                                                                                                                                                  |                                                                                                                                                    
gAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGt                                                                                                                                                   >  4:222866/1‑149 (MQ=255)
   caccacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGGCGATCTCGTCGTgga                                                                                                                                                <  7:35130/149‑2 (MQ=255)
     ccacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGCGGCCTGCGCCCGCTATGACGTGCACCTTATC‑CACGCCGAGATCGCCGTGGGCt                                                                                                                                              >  7:173651/1‑149 (MQ=255)
      cacGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCtt                                                                                                                                             <  3:222866/149‑1 (MQ=255)
         gCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCgg                                                                                                                                          <  8:210182/149‑1 (MQ=255)
                       cGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGTCGCACCGGCACGa                                                                                                                            >  7:205484/1‑149 (MQ=255)
                        gTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGAt                                                                                                                           >  7:122550/1‑149 (MQ=255)
                                    ccGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGa                                                                                                               <  6:61361/149‑1 (MQ=255)
                                          aTACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAg                                                                                                          <  6:173137/148‑1 (MQ=255)
                                                     cGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCATGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGcc                                                                                              >  8:154823/1‑149 (MQ=255)
                                                             tGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTc                                                                                      <  8:122550/149‑1 (MQ=255)
                                                             tGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCATGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTc                                                                                      <  7:154823/149‑1 (MQ=255)
                                                                 cATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGTCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgt                                                                                  <  8:205484/149‑1 (MQ=255)
                                                                 cATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgt                                                                                  <  3:153181/149‑1 (MQ=255)
                                                                 cATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCtgt                                                                                  <  2:222988/149‑1 (MQ=255)
                                                                   tGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGc                                                                                <  5:174165/149‑1 (MQ=255)
                                                                                   ccTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCGCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGTCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCt                                                                  >  1:199253/1‑148 (MQ=255)
                                                                                    cTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTc                                                                                      <  8:101153/126‑1 (MQ=255)
                                                                                    cTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTc                                                                                      >  7:101153/1‑126 (MQ=255)
                                                                                     tCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGc                                                                     <  4:230017/142‑1 (MQ=255)
                                                                                     tCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGc                                                                     >  3:230017/1‑142 (MQ=255)
                                                                                         gttgttGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACcggcg                                                          >  7:249526/1‑149 (MQ=255)
                                                                                              tgCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTAc                                                     >  7:92035/1‑149 (MQ=255)
                                                                                                cgcgAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCt                                                   <  7:253834/149‑1 (MQ=255)
                                                                                                  cgAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGTCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGc                                                 <  2:199253/149‑1 (MQ=255)
                                                                                                       ccTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTg                                            >  6:122276/1‑149 (MQ=255)
                                                                                                        gtGCGACCGCTATGACGTGCAGCTTATC‑CACGTCGAGATCGCCGTGGGCTTCTGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGcc                                                                    <  4:122042/123‑1 (MQ=255)
                                                                                                        cTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGcc                                                                    >  3:122042/1‑124 (MQ=255)
                                                                                                                      aCGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGac                              >  4:143506/1‑148 (MQ=255)
                                                                                                                         tGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGaccacc                          <  8:92035/149‑1 (MQ=255)
                                                                                                                             ccTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctgc                                   <  4:263056/136‑1 (MQ=255)
                                                                                                                             ccTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctgc                                   >  3:263056/1‑136 (MQ=255)
                                                                                                                              gaagcc‑cACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGAc                       >  4:199752/6‑147 (MQ=255)
                                                                                                                                 aTC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgt                  >  4:157803/1‑149 (MQ=255)
                                                                                                                                  tC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgt                  <  1:54814/148‑1 (MQ=255)
                                                                                                                                  cc‑cACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctgcct                                 <  7:243652/132‑1 (MQ=255)
                                                                                                                                  cc‑cACGGCGATCTCGTCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCgcctgcct                                 >  8:243652/2‑133 (MQ=255)
                                                                                                                                        gacgaGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTAc              >  6:203775/1‑147 (MQ=255)
                                                                                                                                          cgaGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgt                  >  2:79037/1‑141 (MQ=255)
                                                                                                                                          cgaGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGgt                  <  1:79037/141‑1 (MQ=255)
                                                                                                                                              aTCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTc                                                                                      <  4:135469/69‑1 (MQ=255)
                                                                                                                                              aTCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTc                                                                                      >  3:135469/1‑69 (MQ=255)
                                                                                                                                                  ccGGGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGGCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGTTCTAt  <  8:173651/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GAACACCACGCCTCGATCAGCGCCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATC‑CACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCCGGATTTCCTGTGCCTGTCCAAGGCCCTGACCGGCGGCTACCTGCCGCTGGCCGCCTGCCTGACCACCGACAAGGTGTACCAGGCGTTCTAT  >  NC_002947/5679002‑5679295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑