Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 461,023 C→A 7.5% R481L (CGG→CTG)  pqqF ← coenzyme PQQ synthesis protein F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947461,0230CA 7.5% 161.5 / 3.6 53R481L (CGG→CTG) pqqFcoenzyme PQQ synthesis protein F
Reads supporting (aligned to +/- strand):  ref base C (24/25);  new base A (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

ACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAG  >  NC_002947/460876‑461170
                                                                                                                                                   |                                                                                                                                                     
aCCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCg                                                                                                                                                      >  7:243142/1‑149 (MQ=255)
   gcggggggCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCgcag                                                                                                                                                   <  4:155433/146‑1 (MQ=255)
     tGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCgcagc‑a                                                                                                                                                <  4:143035/149‑1 (MQ=255)
       ggggCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑Ac                                                                                                                                               <  3:13904/148‑1 (MQ=255)
          gCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑Actgct                                                                                                                                           >  8:139956/1‑149 (MQ=255)
          gCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑Actgct                                                                                                                                           <  6:84982/149‑1 (MQ=255)
               cTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTgg                                                                                                                                      <  3:19822/149‑1 (MQ=255)
               cTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTgg                                                                                                                                      <  4:245107/149‑1 (MQ=255)
                 aCCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGTGCCCTGATGGGGATCATGGACGGGGGCAGCGCCGTGCATGGCAGCCAGCAACTCGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTgg                                                                                                                                      >  1:205176/1‑147 (MQ=255)
                  ccccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGc                                                                                                                                     <  6:177861/147‑1 (MQ=255)
                   cccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAgg                                                                                                                                  >  5:243176/1‑149 (MQ=255)
                           gcgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGgc‑g                                                                                                                           >  5:217967/1‑147 (MQ=255)
                            cgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAg                                                                                                                        <  6:217967/149‑1 (MQ=255)
                              tCTGGCAGTTGCTTGAGCAGGGGCCTGATGCGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCTTCAAGGCCAAGGGTTGTTCCACTGCCCGCAGC‑TCTGCTGCTGGGAGGCCGGC‑GGAGTg                                                                                                                      <  6:167983/149‑1 (MQ=255)
                                  gCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCaa                                                                                                                   <  3:142286/148‑1 (MQ=255)
                                  gCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAAc                                                                                                                  >  3:218235/1‑149 (MQ=255)
                                     gTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGc                                                                                                               <  7:139956/149‑1 (MQ=255)
                                            gAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTAt                                                                                                         <  4:218235/148‑1 (MQ=255)
                                             aGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTc                                                                                                       >  2:70016/1‑149 (MQ=255)
                                               cccggCCCTGATCCCCATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTccc                                                                                                     >  5:233990/4‑149 (MQ=255)
                                               cAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTccc                                                                                                     >  3:2175/1‑149 (MQ=255)
                                                 cggCCCTGATCCCCATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCa                                                                                                                         <  5:8079/126‑1 (MQ=255)
                                                 cggCCCTGATCCCCATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCa                                                                                                                         >  6:8079/2‑127 (MQ=255)
                                                       tGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCActg                                                                                             >  5:42155/1‑149 (MQ=255)
                                                           ggggATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCActgctg                                                                                          >  4:178344/1‑148 (MQ=255)
                                                            gggATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCAACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCActgctg                                                                                          <  1:70016/148‑1 (MQ=255)
                                                             ggATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTAc                                                                                       >  5:275808/1‑149 (MQ=255)
                                                               aTCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTg                                                                                     <  5:197404/149‑1 (MQ=255)
                                                                    ggCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGtt                                                                                >  2:270621/1‑149 (MQ=255)
                                                                     gCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGtt                                                                                <  7:169649/148‑1 (MQ=255)
                                                                         gtggCATCGCGGTGCAGGGCAGCCAGCAACTGTCAGGTGGATTTAGCATCAAGGCCTAGTCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTTCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACg                                                                           <  2:205176/147‑1 (MQ=255)
                                                                           ggCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGa                                                                         <  7:164854/149‑1 (MQ=255)
                                                                            gCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAg                                                                        <  3:178344/149‑1 (MQ=255)
                                                                            gCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAg                                                                        <  6:42155/149‑1 (MQ=255)
                                                                                  ccGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCgg                                                                  <  4:2175/149‑1 (MQ=255)
                                                                                         gggCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATACCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAAc                                                           >  8:146226/1‑149 (MQ=255)
                                                                                          ggCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACg                                                          >  4:191340/1‑149 (MQ=255)
                                                                                             agccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTc                                                       <  6:233990/149‑1 (MQ=255)
                                                                                               ccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCt                                                     <  3:218953/149‑1 (MQ=255)
                                                                                                             gTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAgg                                       >  2:196939/1‑149 (MQ=255)
                                                                                                                  ttGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTg                                  >  7:218951/1‑149 (MQ=255)
                                                                                                                     aGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGtt                                <  1:270621/148‑1 (MQ=255)
                                                                                                                           aaggccaaggcTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGca                          >  2:18256/1‑148 (MQ=255)
                                                                                                                              gccaaggcTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCgg                                                                  >  2:261953/1‑105 (MQ=255)
                                                                                                                              gccaaggcTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCgg                                                                  <  1:261953/105‑1 (MQ=255)
                                                                                                                                         ttCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCt           >  2:70511/1‑149 (MQ=255)
                                                                                                                                            cACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGCGGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTgg         >  1:36440/1‑149 (MQ=255)
                                                                                                                                            cACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGgc        >  3:87058/1‑149 (MQ=255)
                                                                                                                                              cTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGgcg       >  4:38760/1‑148 (MQ=255)
                                                                                                                                               tGCCAGCAGC‑AGTGCTGCGGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTaa                >  2:228758/1‑138 (MQ=255)
                                                                                                                                               tGCCAGCAGC‑AGTGCTGCGGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTaa                <  1:228758/138‑1 (MQ=255)
                                                                                                                                                gCCAGCAGC‑AGTGCTGCGGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCt                    <  1:188643/133‑1 (MQ=255)
                                                                                                                                                gCCAGCAGC‑AGTGCTGCGGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCt                    >  2:188643/1‑133 (MQ=255)
                                                                                                                                                 cccGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCAt    <  8:243142/148‑1 (MQ=255)
                                                                                                                                                  ccGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAg  >  7:217308/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAG  >  NC_002947/460876‑461170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑