Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 461,030 C→G 9.8% V479L (GTG→CTG)  pqqF ← coenzyme PQQ synthesis protein F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947461,0300CG 9.8% 146.8 / 6.4 51V479L (GTG→CTG) pqqFcoenzyme PQQ synthesis protein F
Reads supporting (aligned to +/- strand):  ref base C (24/22);  new base G (3/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

GGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAGGT  >  NC_002947/460883‑461172
                                                                                                                                                    |                                                                                                                                               
ggggCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑Ac                                                                                                                                                 <  3:13904/148‑1 (MQ=255)
   gCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑Actgct                                                                                                                                             <  6:84982/149‑1 (MQ=255)
   gCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑Actgct                                                                                                                                             >  8:139956/1‑149 (MQ=255)
        cTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTgg                                                                                                                                        <  4:245107/149‑1 (MQ=255)
        cTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTgg                                                                                                                                        <  3:19822/149‑1 (MQ=255)
          aCCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGTGCCCTGATGGGGATCATGGACGGGGGCAGCGCCGTGCATGGCAGCCAGCAACTCGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTgg                                                                                                                                        >  1:205176/1‑147 (MQ=255)
           ccccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGc                                                                                                                                       <  6:177861/147‑1 (MQ=255)
            cccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAgg                                                                                                                                    >  5:243176/1‑149 (MQ=255)
                    gcgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGgc‑g                                                                                                                             >  5:217967/1‑147 (MQ=255)
                     cgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAg                                                                                                                          <  6:217967/149‑1 (MQ=255)
                       tCTGGCAGTTGCTTGAGCAGGGGCCTGATGCGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCTTCAAGGCCAAGGGTTGTTCCACTGCCCGCAGC‑TCTGCTGCTGGGAGGCCGGC‑GGAGTg                                                                                                                        <  6:167983/149‑1 (MQ=255)
                           gCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCaa                                                                                                                     <  3:142286/148‑1 (MQ=255)
                           gCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAAc                                                                                                                    >  3:218235/1‑149 (MQ=255)
                              gTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGc                                                                                                                 <  7:139956/149‑1 (MQ=255)
                                     gAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTAt                                                                                                           <  4:218235/148‑1 (MQ=255)
                                      aGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTc                                                                                                         >  2:70016/1‑149 (MQ=255)
                                        cccggCCCTGATCCCCATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTccc                                                                                                       >  5:233990/4‑149 (MQ=255)
                                        cAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTccc                                                                                                       >  3:2175/1‑149 (MQ=255)
                                          cggCCCTGATCCCCATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCa                                                                                                                           >  6:8079/2‑127 (MQ=255)
                                          cggCCCTGATCCCCATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCa                                                                                                                           <  5:8079/126‑1 (MQ=255)
                                                tGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCActg                                                                                               >  5:42155/1‑149 (MQ=255)
                                                    ggggATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCActgctg                                                                                            >  4:178344/1‑148 (MQ=255)
                                                     gggATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCAACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCActgctg                                                                                            <  1:70016/148‑1 (MQ=255)
                                                      ggATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTAc                                                                                         >  5:275808/1‑149 (MQ=255)
                                                        aTCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTg                                                                                       <  5:197404/149‑1 (MQ=255)
                                                             ggCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGtt                                                                                  >  2:270621/1‑149 (MQ=255)
                                                              gCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGtt                                                                                  <  7:169649/148‑1 (MQ=255)
                                                                  gtggCATCGCGGTGCAGGGCAGCCAGCAACTGTCAGGTGGATTTAGCATCAAGGCCTAGTCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTTCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACg                                                                             <  2:205176/147‑1 (MQ=255)
                                                                    ggCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGa                                                                           <  7:164854/149‑1 (MQ=255)
                                                                     gCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAg                                                                          <  3:178344/149‑1 (MQ=255)
                                                                     gCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAg                                                                          <  6:42155/149‑1 (MQ=255)
                                                                           ccGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCgg                                                                    <  4:2175/149‑1 (MQ=255)
                                                                                  gggCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATACCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAAc                                                             >  8:146226/1‑149 (MQ=255)
                                                                                   ggCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACg                                                            >  4:191340/1‑149 (MQ=255)
                                                                                      agccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTc                                                         <  6:233990/149‑1 (MQ=255)
                                                                                        ccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCt                                                       <  3:218953/149‑1 (MQ=255)
                                                                                                      gTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAgg                                         >  2:196939/1‑149 (MQ=255)
                                                                                                           ttGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTg                                    >  7:218951/1‑149 (MQ=255)
                                                                                                              aGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGtt                                  <  1:270621/148‑1 (MQ=255)
                                                                                                                    aaggccaaggcTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGca                            >  2:18256/1‑148 (MQ=255)
                                                                                                                       gccaaggcTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCgg                                                                    >  2:261953/1‑105 (MQ=255)
                                                                                                                       gccaaggcTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCgg                                                                    <  1:261953/105‑1 (MQ=255)
                                                                                                                                  ttCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCt             >  2:70511/1‑149 (MQ=255)
                                                                                                                                     cACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGCGGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTgg           >  1:36440/1‑149 (MQ=255)
                                                                                                                                     cACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGgc          >  3:87058/1‑149 (MQ=255)
                                                                                                                                       cTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGgcg         >  4:38760/1‑148 (MQ=255)
                                                                                                                                        tGCCAGCAGC‑AGTGCTGCGGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTaa                  >  2:228758/1‑138 (MQ=255)
                                                                                                                                        tGCCAGCAGC‑AGTGCTGCGGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTaa                  <  1:228758/138‑1 (MQ=255)
                                                                                                                                         gCCAGCAGC‑AGTGCTGCGGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCt                      <  1:188643/133‑1 (MQ=255)
                                                                                                                                         gCCAGCAGC‑AGTGCTGCGGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCt                      >  2:188643/1‑133 (MQ=255)
                                                                                                                                          cccGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCAt      <  8:243142/148‑1 (MQ=255)
                                                                                                                                          ccagcagc‑aGTGCTGCGGGCAGTCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCa       >  7:7712/4‑147 (MQ=255)
                                                                                                                                           ccGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAg    >  7:217308/1‑149 (MQ=255)
                                                                                                                                              cagc‑aCTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAGGt  >  1:173970/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGC‑ACTGCTGCTGGCAGGCCGGC‑GCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAGGT  >  NC_002947/460883‑461172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑