Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,743,847 A→C 7.4% L673R (CTC→CGC)  glgP ← glycogen phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,743,8470AC 7.4% 152.6 / 2.7 51L673R (CTC→CGC) glgPglycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (24/23);  new base C (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCGGCTATGGCAGCATTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCA  >  NC_002947/5743701‑5743988
                                                                                                                                                  |                                                                                                                                             
aaGCGGCTATGGCAGCATTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCGACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAgc                                                                                                                                             <  7:253161/149‑1 (MQ=255)
         tGGCAGCATTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACTTGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGCGCTCACCATTg                                                                                                                                    <  3:99576/149‑1 (MQ=255)
              gcaTTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAgg                                                                                                                                 >  4:155431/1‑147 (MQ=255)
              gcaTTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAgg                                                                                                                                 <  3:155431/147‑1 (MQ=255)
                        cAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATg                                                                                                                     >  3:219238/1‑149 (MQ=255)
                        cAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATg                                                                                                                     >  2:69553/1‑149 (MQ=255)
                        cAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATg                                                                                                                     >  2:66098/1‑149 (MQ=255)
                             tCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCt                                                                                                                <  7:141575/149‑1 (MQ=255)
                                               gcTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCg                                                                                                <  1:174746/147‑1 (MQ=255)
                                               gcTTCAACCTGCTGCGAGGTCAGGCCAAACATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCg                                                                                                >  2:174746/1‑147 (MQ=255)
                                                 ttCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAAc                                                                                            >  2:36288/1‑149 (MQ=255)
                                                          ctgcGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTc                                                                                                                        <  6:198434/112‑1 (MQ=255)
                                                          ctgcGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTc                                                                                                                        >  5:198434/1‑112 (MQ=255)
                                                          ctgcGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTgg                                                                                   <  4:219238/149‑1 (MQ=255)
                                                            gcGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGa                                                                                  >  2:82072/1‑148 (MQ=255)
                                                            gcGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAACGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTc                                                                                                 >  3:182503/1‑133 (MQ=255)
                                                            gcGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAACGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTc                                                                                                 <  4:182503/133‑1 (MQ=255)
                                                             cGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCAtt                                                                                                                                     >  6:176910/1‑96 (MQ=255)
                                                                      gCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCgg                                                                       <  4:184076/149‑1 (MQ=255)
                                                                        cAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGaa                                                                     <  1:69553/149‑1 (MQ=255)
                                                                        cAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGaa                                                                     <  1:66098/149‑1 (MQ=255)
                                                                         aaaGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGaaa                                                                    <  8:39228/149‑1 (MQ=255)
                                                                          aaGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAg                                                                   >  6:173597/1‑149 (MQ=255)
                                                                          aaGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAg                                                                   <  5:155823/149‑1 (MQ=255)
                                                                           aGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAgg                                                                  >  7:190483/1‑149 (MQ=255)
                                                                              tGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTcc                                                               <  1:82072/149‑1 (MQ=255)
                                                                                 aCATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCg                                                                        >  6:134558/1‑137 (MQ=255)
                                                                                 aCATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCg                                                                        <  5:134558/137‑1 (MQ=255)
                                                                                   aTGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTccgccgc                                                          >  5:37444/1‑149 (MQ=255)
                                                                                     gttgtCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTccgccgccg                                                        >  7:39116/1‑149 (MQ=255)
                                                                                        gtCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGc                                                                                                    <  3:237375/102‑1 (MQ=255)
                                                                                        gtCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGc                                                                                                    >  4:237375/1‑102 (MQ=255)
                                                                                                          gCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGc                                   >  7:71023/1‑149 (MQ=255)
                                                                                                              aTCTCCACTTTTGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTACTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGc                                   <  4:142717/145‑1 (MQ=255)
                                                                                                              aTCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTACTCGGAAAGGTCCGCAGCCGGGATGATGCTCTCGGCCAGGCtag                                >  3:142717/1‑146 (MQ=255)
                                                                                                                       ttGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCgg                                         >  5:37223/1‑130 (MQ=255)
                                                                                                                       ttGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCgg                                         <  6:37223/130‑1 (MQ=255)
                                                                                                                       ttGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGtt                      <  8:71023/149‑1 (MQ=255)
                                                                                                                                tCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGtt                            <  7:7064/134‑1 (MQ=255)
                                                                                                                                tCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGtt                            >  8:7064/1‑134 (MQ=255)
                                                                                                                                  cctggTGCCAATGGTGATCTCACCCTTGAGGCCGAACTTCATGTTGCCGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGGCCGCCGCCGGGATGAGGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAac            <  6:263181/145‑1 (MQ=255)
                                                                                                                                  cAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAaca           >  4:261910/1‑149 (MQ=255)
                                                                                                                                  cAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAaca           >  4:154897/1‑149 (MQ=255)
                                                                                                                                   aGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATc                                                                              >  2:232771/1‑81 (MQ=255)
                                                                                                                                   aGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATc                                                                              <  1:232771/81‑1 (MQ=255)
                                                                                                                                        atcAATGGTGCGCTCACCATTGAGGACGAACTACAAGTTGCTGGTACCGGAACCTTCGTAACCAGCCTTGGAAATCTGCTCGGAAAGCTCCGCCGCCGGGATGATGCTCTCGCCCAGGCTGACGTTCTAGATGGGCAGGAACTCCACCt     >  3:91250/3‑149 (MQ=255)
                                                                                                                                         ccAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCtt    >  7:39746/1‑149 (MQ=255)
                                                                                                                                          cAATGGTGCGCTCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGtt                      <  4:118939/130‑1 (MQ=255)
                                                                                                                                          cAATGGTGCGCTCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGtt                      >  3:118939/1‑130 (MQ=255)
                                                                                                                                           aaTGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTg                           <  4:255698/124‑1 (MQ=255)
                                                                                                                                           aaTGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTca  <  8:190483/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
AAGCGGCTATGGCAGCATTGGCGCCAAAATCACCGGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCA  >  NC_002947/5743701‑5743988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑