Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,753,485	T→G	11.5%	intergenic (+46/‑320)	glnA → / → glnL	glutamine synthetase/two‑component system sensor histidine kinase/phosphatase GlnL/GlnG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,753,485	0	T	G	11.5%	189.9 / 12.9	68	intergenic (+46/‑320)	glnA/glnL	glutamine synthetase/two‑component system sensor histidine kinase/phosphatase GlnL/GlnG
Reads supporting (aligned to +/- strand):  ref base T (32/28);  new base G (5/3);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

CGACTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGTTTCACCAAGACTTTC  >  NC_002947/5753340‑5753631                                                                                                                                                   |                                                                                                                                                    cgacTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCgg                                                                                                                                                  <  5:43551/149‑1 (MQ=255) cgacTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCgg                                                                                                                                                  <  3:253922/149‑1 (MQ=255) cgacTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCgg                                                                                                                                                  <  3:119917/149‑1 (MQ=255)   acTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGa                                                                                                                                                <  3:238156/149‑1 (MQ=255)     ttCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCgagggagg                                                                                                                                              >  8:61361/1‑149 (MQ=255)     ttCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAgg                                                                                                                                              >  1:26247/1‑149 (MQ=255)     ttCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAgg                                                                                                                                              >  6:268674/1‑149 (MQ=255)       cATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGcc                                                                                                                                            >  3:189422/1‑149 (MQ=255)       cATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCACGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGc                                                                                                                                             <  1:16582/148‑1 (MQ=255)        aTCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGAGGGAGGCCg                                                                                                                                           <  7:61361/149‑1 (MQ=255)           gATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGttt                                                                                                                                        >  7:57425/1‑149 (MQ=255)           gATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGAGGGAGGCCGtt                                                                                                                                         <  8:141255/148‑1 (MQ=255)               ccTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGt                                                                                                                                    <  2:26247/149‑1 (MQ=255)                cTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACAGCCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGt                                                                                                                                    >  1:202680/1‑149 (MQ=255)                 ttCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGttt                                                                                                                                        >  1:110240/1‑143 (MQ=255)                 ttCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGttt                                                                                                                                        <  2:110240/143‑1 (MQ=255)                             aaGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTg                                                                                                                      >  6:18311/1‑149 (MQ=255)                               gagCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCa                                                                                                                    <  8:57425/149‑1 (MQ=255)                                agCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTg                                                                                                                               >  4:260118/1‑137 (MQ=255)                                agCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTg                                                                                                                               <  3:260118/137‑1 (MQ=255)                                    aagaagaaATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAgc                                                                                                               >  8:198671/1‑149 (MQ=255)                                    aagaagaaATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGATGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCATCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCATAAgc                                                                                                               >  8:137793/1‑149 (MQ=255)                                     agaagaaATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTg                                                                                                                      >  4:144707/1‑141 (MQ=255)                                     agaagaaATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTg                                                                                                                      <  3:144707/141‑1 (MQ=255)                                      gaagaaATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAgcgc                                                                                                             <  2:100640/149‑1 (MQ=255)                                         gaaATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGc                                                                                                                     <  2:233734/138‑1 (MQ=255)                                         gaaATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCG‑TTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCt                                                                                                         <  5:268674/149‑1 (MQ=255)                                          aaaTCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCt                                                                                                         >  5:102797/1‑149 (MQ=255)                                            aTCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGa                                                                                                       <  3:7685/149‑1 (MQ=255)                                                        aCCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAg                                                                                                                <  6:115971/128‑1 (MQ=255)                                                        aCCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAg                                                                                                                >  5:115971/1‑128 (MQ=255)                                                          cTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGGCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGc                                                                                         >  4:1852/1‑149 (MQ=255)                                                                aca‑cCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAgc                                                                                                               <  6:91248/121‑1 (MQ=255)                                                                     ccGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATg                                                                                     >  3:37188/1‑143 (MQ=255)                                                                     ccGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATg                                                                                     <  4:37188/143‑1 (MQ=255)                                                                     ccGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTAt                                                                               >  5:137192/1‑149 (MQ=255)                                                                      cGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATc                                                                              >  8:89653/1‑149 (MQ=255)                                                                       gCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCt                                                                             >  2:2966/1‑149 (MQ=255)                                                                         tGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTg                                                                           >  4:66827/1‑149 (MQ=255)                                                                                cGAGCTGTACTACAGCTGCTGATGTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCAt                                                                     <  5:18311/148‑1 (MQ=255)                                                                                      gTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTca                                                              >  5:134183/1‑149 (MQ=255)                                                                                          tacAGCTGATGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCAGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCagac                                                           >  7:82752/1‑148 (MQ=255)                                                                                                  ctgatctgatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCt                                                   <  6:137192/148‑1 (MQ=255)                                                                                                    gatctgatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCaa                                                >  4:232329/1‑149 (MQ=255)                                                                                                        tgatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGc                                            >  2:134823/1‑149 (MQ=255)                                                                                                         gatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCt                                           >  8:116889/1‑149 (MQ=255)                                                                                                         gatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCt                                           >  8:120349/1‑149 (MQ=255)                                                                                                           ttGGCGCCTCGCGCCGATGACATGATCAAAACTGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCTCACCTTGCAGAATCGCCGCTGATTCGCCTGTCACGCTATGATCTATCTTGGTGCATTAATTAACACCATGCCCTCAATTGCTcc                                         >  8:200050/1‑149 (MQ=255)                                                                                                            tGGCGGCTGGAGCCGATGACTTTATAAAAACGGACTCACTCTTGAGGCCTTTTTTGTTTCTGCTCAGATTGAATAATCGCCGGTGATTCGCCTGTCACGCTATTCTATATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTcc                                         <  7:200050/148‑1 (MQ=255)                                                                                                                  cTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGc                                                                                                  >  3:14912/1‑85 (MQ=255)                                                                                                                  cTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGc                                                                                                  <  4:14912/85‑1 (MQ=255)                                                                                                                       gcCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGtt                              >  6:270676/1‑148 (MQ=255)                                                                                                                            tGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGtt                              >  8:54303/1‑143 (MQ=255)                                                                                                                            tGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGtt                              <  7:54303/143‑1 (MQ=255)                                                                                                                                aTGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑TAGGTTAAAc                   <  7:198671/149‑1 (MQ=255)                                                                                                                                 tGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCt                                                                             >  2:142152/1‑91 (MQ=255)                                                                                                                                 tGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCt                                                                             <  1:142152/91‑1 (MQ=255)                                                                                                                                  gATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGt                 >  6:59996/1‑149 (MQ=255)                                                                                                                                   acaaaaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGGACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTATTTCACACCATGCGCTCAATTGCTCCACAATCTGGTT‑AAGGTTAAACGtt                >  8:252334/4‑149 (MQ=255)                                                                                                                                   aTCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAACGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑c                            >  5:167834/1‑137 (MQ=255)                                                                                                                                   aTCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAACGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑c                            <  6:167834/137‑1 (MQ=255)                                                                                                                                    tCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGttt               <  3:66827/149‑1 (MQ=255)                                                                                                                                    tCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGtt                <  3:232329/148‑1 (MQ=255)                                                                                                                                     aaaaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACAGCATGCCCTCAATTGCTCCCCAATCTGGTC‑CAGGTTAAACGttt               >  7:101861/2‑148 (MQ=255)                                                                                                                                      aaaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGTTTCa             >  2:212378/1‑149 (MQ=255)                                                                                                                                      aaaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGTTTCa             >  4:22604/1‑149 (MQ=255)                                                                                                                                       aaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGTTTCAc            <  3:22604/149‑1 (MQ=255)                                                                                                                                        aaCGGCCTCCCTCGTGAGTCCGTTTTTGTTTCTGCGCACCTTTCATAAGCGCCGATGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGTTTCAcc           <  7:137793/149‑1 (MQ=255)                                                                                                                                         aCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGTTTCAcc           >  6:92546/1‑148 (MQ=255)                                                                                                                                                 cctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCCAGGTTAAACGTTTCACCAAGACttt   <  1:2966/149‑1 (MQ=255)                                                                                                                                                 cctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGTTTCACCAAGACTTTc  <  7:89653/149‑1 (MQ=255)                                                                                                                                                 cctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGTTTCACCAAGACTTTc  <  6:134183/149‑1 (MQ=255)                                                                                                                                                 cctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGTTTCACCAAGACTTTc  <  6:102797/149‑1 (MQ=255)                                                                                                                                                 cctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGTTTCACCAAGACTTTc  <  5:270676/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CGACTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACA‑CCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTT‑CAGGTTAAACGTTTCACCAAGACTTTC  >  NC_002947/5753340‑5753631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑