Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,765,710 C→A 8.3% S122R (AGC→AGA)  PP_5059 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,765,7100CA 8.3% 149.9 / 2.2 48S122R (AGC→AGA) PP_5059hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (20/24);  new base A (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GACGGTGATCCTGCACATGCCCATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCACGGTTGCCGCGGCCGAGGCTCAAGCACACGATGTCGCGCATGTATCACGG  >  NC_002947/5765563‑5765854
                                                                                                                                                    |                                                                                                                                                
gACGGTGATCCTGCACATGCCCATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGcc                                                                                                                                                 <  5:212780/149‑1 (MQ=255)
   ggTGATCCTGCACATGCCCATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATCGGACATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGACg                                                                                                                                                >  1:77260/1‑148 (MQ=255)
   ggTGATCCTGCACATGCCCATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATCAGACATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGACg                                                                                                                                                >  1:80673/1‑148 (MQ=255)
    gTGATCCTGCACATGCCCATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCACCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCAt                                                                                                                                             <  6:176687/149‑1 (MQ=255)
           tGCACATGCCCATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTCCGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGca                                                                                                                                      >  5:207683/1‑149 (MQ=255)
           tGCACATGCCCATGGACCCGGCCACCAGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGca                                                                                                                                      >  3:147395/1‑149 (MQ=255)
                aTGCCCATGGGCCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCg                                                                                                                                 >  2:238935/1‑149 (MQ=255)
                aTGCCCATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCg                                                                                                                                 <  6:59566/149‑1 (MQ=255)
                 tGCCCATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGt                                                                                                                                <  5:56181/149‑1 (MQ=255)
                  gCCCATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGt                                                                                                                                >  6:247563/1‑148 (MQ=255)
                   cccATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGa                                                                                                                              <  5:136959/149‑1 (MQ=255)
                    ccATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGaa                                                                                                                             <  8:116060/149‑1 (MQ=255)
                     cATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAAc                                                                                                                            <  5:195112/149‑1 (MQ=255)
                               gCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGACGCATGACCGCACAGCGTGAACCCATGGCGTg                                                                                                                  <  2:77260/149‑1 (MQ=255)
                               gCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGACGCATGACCGCACAGCGTGAACCCATGGCGTg                                                                                                                  <  2:80673/149‑1 (MQ=255)
                                  aCCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGtt                                                                                                               <  6:184979/149‑1 (MQ=255)
                                     aggCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGAt                                                                                                            <  4:147395/148‑1 (MQ=255)
                                             tGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGc                                                                                                    <  5:247563/149‑1 (MQ=255)
                                                    cACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAgcg                                                                                             <  1:212978/149‑1 (MQ=255)
                                                     aCCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATg                                                                                                                       <  4:157107/122‑1 (MQ=255)
                                                     aCCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATg                                                                                                                       >  3:157107/1‑122 (MQ=255)
                                                     aCCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAgcgg                                                                                            >  4:54438/1‑149 (MQ=255)
                                                      cccGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAgcggc                                                                                           <  7:10088/149‑1 (MQ=255)
                                                       ccGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATg                                                                                                           <  5:252042/132‑1 (MQ=255)
                                                       ccGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATg                                                                                                           >  6:252042/1‑132 (MQ=255)
                                                         gggCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGcc                                                                                        <  1:238935/149‑1 (MQ=255)
                                                                 gCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCa                                                                                                <  8:186038/134‑1 (MQ=255)
                                                                 gCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCa                                                                                                >  7:186038/1‑134 (MQ=255)
                                                                       gAAGAGCTGGTGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTg                                                                            <  5:7759/148‑1 (MQ=255)
                                                                        aaGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTc                                                                          <  3:54438/149‑1 (MQ=255)
                                                                        aaGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTc                                                                          >  2:113957/1‑149 (MQ=255)
                                                                           agCTGGCGCGGCGCCTGGACGCAGCATTGGACAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGa                                                                        >  1:25226/1‑148 (MQ=255)
                                                                             cTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGc                                                                                                    <  5:14575/118‑1 (MQ=255)
                                                                             cTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGc                                                                                                    >  6:14575/1‑118 (MQ=255)
                                                                                               gcagcaTTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCAc                                                   >  1:35285/1‑149 (MQ=255)
                                                                                                            aGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCACGGTTGCCGCGGcc                                      >  6:96838/1‑149 (MQ=255)
                                                                                                               tGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCACGGTTGCCGCGGCCGAg                                   <  6:238603/149‑1 (MQ=255)
                                                                                                                      cgccgcCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCACGGTTGCCGCGGCCGAGGCTCAAg                            <  2:25226/149‑1 (MQ=255)
                                                                                                                            cgGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCCCGGTTGCCGCGGCCGAGGCTCAAGCacac                       >  2:254973/1‑148 (MQ=255)
                                                                                                                                  caacaaCCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCACGGTTGCCGCGGCCGAGGCTCAAGCACACGATGTcg                >  5:67755/1‑149 (MQ=255)
                                                                                                                                   aacaacCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCACGGTTGCCGCGGCCGAGGCTCAAGCACACGATGTcgc               <  2:35285/149‑1 (MQ=255)
                                                                                                                                    acaacCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCAt                                                                                      >  7:112708/1‑77 (MQ=255)
                                                                                                                                    acaacCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCAt                                                                                      <  8:112708/77‑1 (MQ=255)
                                                                                                                                     caacCACATGGGTACCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTCATGGGCGAGCTGCACCGGAGCCATCTGTACTTTGTCCACAGCCCCACCACTGCCGCCACGGTTGCAGCGCCCGAGCCTCAAGCACACGATGTcgcc              >  8:27904/1‑147 (MQ=255)
                                                                                                                                      aacCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCACGGTTGCCGCGGCCGAGGCTCAAGCACACGATGTCGCGCa            <  5:96838/149‑1 (MQ=255)
                                                                                                                                        ccACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCACGGTTGCCGCGGCCGAGGCTCAAGCACACGATGTCGCGCATg          >  2:146797/1‑149 (MQ=255)
                                                                                                                                          acaTGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCACGGTTGCCGCGGCCGAgg                                  >  5:212836/1‑123 (MQ=255)
                                                                                                                                          acaTGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCACGGTTGCCGCGGCCGAgg                                  <  6:212836/123‑1 (MQ=255)
                                                                                                                                                 tAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCACGGTTGCCGCGGCCGAGGCTCAAGCACACGATGTCGCGCATGTATCACgg  >  6:71848/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GACGGTGATCCTGCACATGCCCATGGACCCGGCCACCGGGCCCTATGCCTGGCACCCGGGCATC‑GCCATCGAAGAGCTGGCGCGGCGCCTGGACGCAGCATTGGTCAAGGTGCCCTACGCCGCCGGCATCAACAACCACATGGGTAGCCGCATGACCGCACAGCGTGAACCCATGGCGTGGTTGATGGGCGAGCTGCAGCGGCGCCATCTGTTCTTTGTCGACAGCCGCACCAGTGCCGCCACGGTTGCCGCGGCCGAGGCTCAAGCACACGATGTCGCGCATGTATCACGG  >  NC_002947/5765563‑5765854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑