Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,808,008	C→G	10.4%	intergenic (+143/+12)	mrcA → / ← maeB	penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase/malic enzyme B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,808,008	0	C	G	10.4%	135.2 / 6.9	48	intergenic (+143/+12)	mrcA/maeB	penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase/malic enzyme B
Reads supporting (aligned to +/- strand):  ref base C (18/25);  new base G (3/2);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

TTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGTGATCAAGCGTGCATCCAGCGGCTTC  >  NC_002947/5807860‑5808155                                                                                                                                                     |                                                                                                                                                    ttCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTc                                                                                                                                                     >  2:36449/1‑149 (MQ=255)     aaGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCtt                                                                                                                                                 >  3:85007/1‑149 (MQ=255)      aGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCtt                                                                                                                                                 <  6:204629/148‑1 (MQ=255)      aGGCAGGGCGCCTGACTCAGGGTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCt                                                                                                                                                  <  5:277542/147‑1 (MQ=255)              cgcCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATc                                                                                                                                        <  4:158002/149‑1 (MQ=255)               gcCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCGCATGAGCCGGGGCTTTTTTATGGGGCTtgggaatgt                                                                                                                                         >  8:151700/1‑140 (MQ=25)               gcCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCGCATGAGCCGGGGCTTTTTTATGGGGCTtgggaatgt                                                                                                                                         <  7:151700/147‑8 (MQ=25)                    aCTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGcc                                                                                                                                    <  7:146205/147‑1 (MQ=255)                     cTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGCGCATTTTTATTTCGCTTGCAGCAATCATCCGt                                                                                                                                  >  2:248215/1‑148 (MQ=255)                      tCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGtt                                                                                                                                 >  1:93323/1‑148 (MQ=255)                      tCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGtt                                                                                                                                 >  5:39609/1‑148 (MQ=255)                       cAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTg                                                                                                                                >  7:187011/1‑148 (MQ=255)                        aGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGaa                                                                                                                              <  6:39609/149‑1 (MQ=255)                           ttttGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAacac                                                                                                                           >  5:60606/1‑149 (MQ=255)                              tGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACtt                                                                                                                         <  1:36449/148‑1 (MQ=255)                                cGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGACCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCa                                                                                                                       <  1:248215/148‑1 (MQ=255)                                 gTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCAt                                                                                                                      >  1:270771/1‑148 (MQ=255)                                        cTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTg                                                                                                              <  5:118714/149‑1 (MQ=255)                                        cTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTg                                                                                                              >  6:153924/1‑149 (MQ=255)                                                   aGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGCCACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGGTGAACACTTCATCCACGCTGGTCAGCGGGt                                                                                                    >  8:122127/1‑148 (MQ=255)                                                     gcgcCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAAt                                                                                                 <  5:266370/149‑1 (MQ=255)                                                      cgcCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATg                                                                                                <  8:187011/149‑1 (MQ=255)                                                       gcCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGc                                                                                               <  4:85007/149‑1 (MQ=255)                                                          acaAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTc                                                                                            <  2:179112/149‑1 (MQ=255)                                                          acaAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTc                                                                                            <  2:270771/149‑1 (MQ=255)                                                               tGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATa                                                                                       <  6:60606/149‑1 (MQ=255)                                                                  ccTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAgg                                                                                    >  4:163202/1‑149 (MQ=255)                                                                       ccAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAgg                                                                                <  3:163202/148‑1 (MQ=255)                                                                        cAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTc                                                                                            >  5:174734/1‑135 (MQ=255)                                                                        cAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTc                                                                                            <  6:174734/135‑1 (MQ=255)                                                                        cAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAggg                                                                               >  1:185937/1‑148 (MQ=255)                                                                                        tGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCg                                                              >  3:97200/1‑149 (MQ=255)                                                                                          acacATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGAt                                                            <  2:61692/149‑1 (MQ=255)                                                                                                tCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGc                                                      >  1:244559/1‑149 (MQ=255)                                                                                                       ccccATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGc                                                >  4:110478/1‑148 (MQ=255)                                                                                                        gacATAAAAAAGCCCCGGCTCGCATGAGCCGGGGCTTTTTTATGGGGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCca                                              >  1:101113/3‑149 (MQ=37)                                                                                                            tAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGc                                          <  6:199993/149‑1 (MQ=255)                                                                                                               aaaaGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTAATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATc                                       >  8:145205/1‑149 (MQ=255)                                                                                                                   gCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGaa                                   <  3:110478/149‑1 (MQ=255)                                                                                                                    ccccGGCTCGCATGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGaaa                                  >  3:95762/1‑149 (MQ=255)                                                                                                                    ccccGGCTCGCATGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGaaa                                  <  4:95762/149‑1 (MQ=255)                                                                                                                    ccccGGCTCGCATGAGCCGGGGCTTTTTTATGGGGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAg                                           <  5:33578/140‑1 (MQ=38)                                                                                                                    ccccGGCTCGCATGAGCCGGGGCTTTTTTATGGGGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAg                                           >  6:33578/1‑140 (MQ=38)                                                                                                                          cTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACgg                            <  5:153924/149‑1 (MQ=255)                                                                                                                             aTGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGt                           <  4:97200/147‑1 (MQ=255)                                                                                                                                     agtggattttttATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCGGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGTGATCAAGCGt                 <  8:78426/143‑1 (MQ=255)                                                                                                                                                    tCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGTGATCAAGCGTGCATCCAGCGGCTTc  >  7:222594/1‑149 (MQ=255)                                                                                                                                                    tCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGTGATCAAGCGTGCATCCAGCGGCTTc  <  2:244559/149‑1 (MQ=255)                                                                                                                                                    tCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGTGATCAAGCGTGCATCCAGCGGCTTc  <  2:185937/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGTGATCAAGCGTGCATCCAGCGGCTTC  >  NC_002947/5807860‑5808155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑