Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,824,712 T→G 8.5% intergenic (+136/+38) PP_5102 → / ← trmB hypothetical protein/tRNA (guanosine(46)‑N7)‑methyltransferase TrmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,824,7120TG 8.5% 170.2 / 6.4 59intergenic (+136/+38)PP_5102/trmBhypothetical protein/tRNA (guanosine(46)‑N7)‑methyltransferase TrmB
Reads supporting (aligned to +/- strand):  ref base T (27/27);  new base G (2/3);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

CGCGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCC  >  NC_002947/5824568‑5824857
                                                                                                                                                |                                                                                                                                                   
cgcgGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCtttt                                                                                                                                                   >  2:256613/1‑147 (MQ=255)
     cTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCg                                                                                                                                            <  6:177954/149‑1 (MQ=255)
        aGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGtt                                                                                                                                         <  6:67933/149‑1 (MQ=255)
         gCGAATGCCCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGttt                                                                                                                                        <  3:3298/149‑1 (MQ=21)
         gCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGttt                                                                                                                                        >  1:44267/1‑149 (MQ=255)
           gAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTT‑AGGGCGGTTTTAc                                                                                                                                     >  5:114301/1‑149 (MQ=255)
            aaTGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAc                                                                                                                                     >  1:53143/1‑149 (MQ=255)
             aTGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAcc                                                                                                                                    >  1:225177/1‑149 (MQ=255)
              tGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGttttttct                                                                                                                                   <  5:38744/149‑5 (MQ=21)
                 ccccTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGttttttctttg                                                                                                                                <  2:44267/149‑8 (MQ=18)
                    cTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAgg                                                                                                                             >  7:65874/1‑149 (MQ=255)
                    cTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAgg                                                                                                                             <  1:256613/149‑1 (MQ=255)
                        tAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTT‑AGGGCGGTTTTACCGAAAAGGCCGt                                                                                                                         <  6:114301/148‑1 (MQ=255)
                              cGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCg                                                                                                                                            <  4:262534/124‑1 (MQ=255)
                              cGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCg                                                                                                                                            >  3:262534/1‑124 (MQ=255)
                                gATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCg                                                                                                                 >  3:249720/1‑149 (MQ=255)
                                 aTTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGc                                                                                                                <  5:107173/149‑1 (MQ=255)
                                 aTTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGc                                                                                                                >  6:107173/1‑149 (MQ=255)
                                 aTTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGc                                                                                                                >  7:140667/1‑149 (MQ=255)
                                       ccGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCCGCCGCTCCATc                                                                                                          >  3:214828/1‑149 (MQ=255)
                                       ccGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATc                                                                                                          >  1:99714/1‑149 (MQ=255)
                                       ccGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATc                                                                                                          <  6:201703/149‑1 (MQ=255)
                                           gAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCAcc                                                                                                      >  4:138472/1‑149 (MQ=255)
                                                    ggccCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAAc                                                                                             >  7:28024/1‑149 (MQ=255)
                                                    ggccCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAAc                                                                                             <  2:53143/149‑1 (MQ=255)
                                                           ctgGCCAATCTCCACCCTCCCTTCACCGTGGGCGCCGCTAGCTTATTCGCGGGTAAACGCGCTCCTACAAAGAAAAAACCTCCCTTTTCAGGGCGGTTTTAGCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCaa                                                                                       <  1:115077/147‑1 (MQ=255)
                                                              gCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGGTCAATCCACCTTCTCGAACT‑TCAAGT‑cc                                                                                  <  1:151983/148‑1 (MQ=255)
                                                                cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑Cacac                                                                               >  2:202678/1‑149 (MQ=255)
                                                                cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCcac                                                                               <  3:255216/149‑1 (MQ=255)
                                                                cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCcac                                                                               >  8:19986/1‑149 (MQ=255)
                                                                cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCca                                                                                >  2:206039/1‑148 (MQ=255)
                                                                 aaTCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCt                                                                                                               >  6:63743/1‑118 (MQ=255)
                                                                 aaTCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCt                                                                                                               <  5:63743/118‑1 (MQ=255)
                                                                  aTCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCacac                                                                             >  7:5297/1‑149 (MQ=255)
                                                                      ccACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCg                                                                         >  6:254523/1‑149 (MQ=255)
                                                                       cACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCCCACCCCGt                                                                        >  4:194109/1‑149 (MQ=255)
                                                                       cACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGt                                                                        <  2:225177/149‑1 (MQ=255)
                                                                        aCCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTg                                                                       >  8:196948/1‑149 (MQ=255)
                                                                         cccGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGc                                                                      <  8:65874/149‑1 (MQ=255)
                                                                           cGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGccc                                                                    >  8:99998/1‑149 (MQ=255)
                                                                                  cACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTCTCAAGT‑CCCACACCCCGTGCCCCAGc                                                                <  1:141012/147‑1 (MQ=255)
                                                                                  cACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGcc                                                               <  2:99714/147‑1 (MQ=255)
                                                                                   aCCGTGGTCGCCGCTACCTTATTCGCGGTTAAACCCGCTACTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGc                                                             <  4:214828/148‑1 (MQ=255)
                                                                                   aCCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGc                                                             <  4:196794/148‑1 (MQ=255)
                                                                                    ccGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACAcc                                                                            <  4:242457/132‑1 (MQ=255)
                                                                                    ccGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACAcc                                                                            >  3:242457/1‑132 (MQ=255)
                                                                                                   ccGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCg                                              <  8:28024/147‑1 (MQ=255)
                                                                                                      tATTCGCGGGTAAACCCGCTACTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACtt                                         >  6:62157/1‑149 (MQ=255)
                                                                                                                tAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGTCCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCgg                                <  1:202678/149‑1 (MQ=255)
                                                                                                                tAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGTCCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCgg                                <  1:206039/149‑1 (MQ=255)
                                                                                                                           cTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCgg                     <  5:254523/148‑1 (MQ=255)
                                                                                                                             aCAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgc                  >  2:176830/1‑149 (MQ=255)
                                                                                                                               aaaGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgcgc                >  3:69211/1‑149 (MQ=255)
                                                                                                                                  gAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGc             <  8:140667/149‑1 (MQ=255)
                                                                                                                                   gtaaaaCCGCCCTGAAAAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGca            >  1:100097/3‑149 (MQ=255)
                                                                                                                                    aaaaaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGca            >  1:131178/1‑148 (MQ=255)
                                                                                                                                       aaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATa        <  7:99998/149‑1 (MQ=255)
                                                                                                                                          cGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGt     >  7:214557/1‑149 (MQ=255)
                                                                                                                                          cGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGt     <  7:196948/149‑1 (MQ=255)
                                                                                                                                             ccTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGcc  <  8:5297/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CGCGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCC  >  NC_002947/5824568‑5824857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑