Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,824,715 C→A 8.6% intergenic (+139/+35) PP_5102 → / ← trmB hypothetical protein/tRNA (guanosine(46)‑N7)‑methyltransferase TrmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,824,7150CA 8.6% 175.1 / 6.8 60intergenic (+139/+35)PP_5102/trmBhypothetical protein/tRNA (guanosine(46)‑N7)‑methyltransferase TrmB
Reads supporting (aligned to +/- strand):  ref base C (26/27);  new base A (2/3);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

CTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTC  >  NC_002947/5824573‑5824860
                                                                                                                                              |                                                                                                                                                   
cTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCg                                                                                                                                               <  6:177954/149‑1 (MQ=255)
   aGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGtt                                                                                                                                            <  6:67933/149‑1 (MQ=255)
    gCGAATGCCCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGttt                                                                                                                                           <  3:3298/149‑1 (MQ=21)
    gCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGttt                                                                                                                                           >  1:44267/1‑149 (MQ=255)
      gAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTT‑AGGGCGGTTTTAc                                                                                                                                        >  5:114301/1‑149 (MQ=255)
       aaTGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAc                                                                                                                                        >  1:53143/1‑149 (MQ=255)
        aTGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTAcc                                                                                                                                       >  1:225177/1‑149 (MQ=255)
         tGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGttttttct                                                                                                                                      <  5:38744/149‑5 (MQ=21)
            ccccTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGGCGGttttttctttg                                                                                                                                   <  2:44267/149‑8 (MQ=18)
               cTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAgg                                                                                                                                >  7:65874/1‑149 (MQ=255)
               cTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAgg                                                                                                                                <  1:256613/149‑1 (MQ=255)
                   tAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTT‑AGGGCGGTTTTACCGAAAAGGCCGt                                                                                                                            <  6:114301/148‑1 (MQ=255)
                         cGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCg                                                                                                                                               <  4:262534/124‑1 (MQ=255)
                         cGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCg                                                                                                                                               >  3:262534/1‑124 (MQ=255)
                           gATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCg                                                                                                                    >  3:249720/1‑149 (MQ=255)
                            aTTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGc                                                                                                                   <  5:107173/149‑1 (MQ=255)
                            aTTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGc                                                                                                                   >  6:107173/1‑149 (MQ=255)
                            aTTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGc                                                                                                                   >  7:140667/1‑149 (MQ=255)
                                  ccGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCCGCCGCTCCATc                                                                                                             >  3:214828/1‑149 (MQ=255)
                                  ccGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATc                                                                                                             >  1:99714/1‑149 (MQ=255)
                                  ccGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATc                                                                                                             <  6:201703/149‑1 (MQ=255)
                                      gAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCAcc                                                                                                         >  4:138472/1‑149 (MQ=255)
                                               ggccCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAAc                                                                                                >  7:28024/1‑149 (MQ=255)
                                               ggccCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAAc                                                                                                <  2:53143/149‑1 (MQ=255)
                                                      ctgGCCAATCTCCACCCTCCCTTCACCGTGGGCGCCGCTAGCTTATTCGCGGGTAAACGCGCTCCTACAAAGAAAAAACCTCCCTTTTCAGGGCGGTTTTAGCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCaa                                                                                          <  1:115077/147‑1 (MQ=255)
                                                         gCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGGTCAATCCACCTTCTCGAACT‑TCAAGT‑cc                                                                                     <  1:151983/148‑1 (MQ=255)
                                                           cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑Cacac                                                                                  >  2:202678/1‑149 (MQ=255)
                                                           cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCcac                                                                                  <  3:255216/149‑1 (MQ=255)
                                                           cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCcac                                                                                  >  8:19986/1‑149 (MQ=255)
                                                           cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCca                                                                                   >  2:206039/1‑148 (MQ=255)
                                                            aaTCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCt                                                                                                                  >  6:63743/1‑118 (MQ=255)
                                                            aaTCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCt                                                                                                                  <  5:63743/118‑1 (MQ=255)
                                                             aTCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCacac                                                                                >  7:5297/1‑149 (MQ=255)
                                                                 ccACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCg                                                                            >  6:254523/1‑149 (MQ=255)
                                                                  cACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCCCACCCCGt                                                                           >  4:194109/1‑149 (MQ=255)
                                                                  cACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGt                                                                           <  2:225177/149‑1 (MQ=255)
                                                                   aCCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTg                                                                          >  8:196948/1‑149 (MQ=255)
                                                                    cccGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGc                                                                         <  8:65874/149‑1 (MQ=255)
                                                                      cGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGccc                                                                       >  8:99998/1‑149 (MQ=255)
                                                                             cACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTCTCAAGT‑CCCACACCCCGTGCCCCAGc                                                                   <  1:141012/147‑1 (MQ=255)
                                                                             cACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGcc                                                                  <  2:99714/147‑1 (MQ=255)
                                                                              aCCGTGGTCGCCGCTACCTTATTCGCGGTTAAACCCGCTACTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGc                                                                <  4:214828/148‑1 (MQ=255)
                                                                              aCCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGc                                                                <  4:196794/148‑1 (MQ=255)
                                                                               ccGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACAcc                                                                               <  4:242457/132‑1 (MQ=255)
                                                                               ccGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACAcc                                                                               >  3:242457/1‑132 (MQ=255)
                                                                                              ccGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCg                                                 <  8:28024/147‑1 (MQ=255)
                                                                                                 tATTCGCGGGTAAACCCGCTACTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACtt                                            >  6:62157/1‑149 (MQ=255)
                                                                                                           tAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGTCCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCgg                                   <  1:202678/149‑1 (MQ=255)
                                                                                                           tAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGTCCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCgg                                   <  1:206039/149‑1 (MQ=255)
                                                                                                                      cTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCgg                        <  5:254523/148‑1 (MQ=255)
                                                                                                                        aCAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgc                     >  2:176830/1‑149 (MQ=255)
                                                                                                                          aaaGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgcgc                   >  3:69211/1‑149 (MQ=255)
                                                                                                                             gAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGc                <  8:140667/149‑1 (MQ=255)
                                                                                                                              gtaaaaCCGCCCTGAAAAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGca               >  1:100097/3‑149 (MQ=255)
                                                                                                                               aaaaaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGca               >  1:131178/1‑148 (MQ=255)
                                                                                                                                  aaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATa           <  7:99998/149‑1 (MQ=255)
                                                                                                                                     cGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGt        >  7:214557/1‑149 (MQ=255)
                                                                                                                                     cGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGt        <  7:196948/149‑1 (MQ=255)
                                                                                                                                        ccTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGcc     <  8:5297/149‑1 (MQ=255)
                                                                                                                                            ttCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTc  <  8:214557/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACT‑TCAAGT‑CCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTC  >  NC_002947/5824573‑5824860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑