Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,830,803	G→A	6.4%	G447G (GGC→GGT)	ftsY ←	signal recognition particle protein translocation receptor GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,830,803	0	G	A	6.4%	188.0 / 2.2	62	G447G (GGC→GGT)	ftsY	signal recognition particle protein translocation receptor GTPase
Reads supporting (aligned to +/- strand):  ref base G (30/28);  new base A (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

TCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTCGTGCGGCGCCTCAACGTCGAGCTTGCCGATGACCCGG  >  NC_002947/5830659‑5830950                                                                                                                                                 |                                                                                                                                                     tCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGt                                                                                                                                                  >  2:140796/1‑149 (MQ=255)   aaTCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTcc                                                                                                                                                <  8:183004/149‑1 (MQ=255)    aTCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCa                                                                                                                                               <  1:140796/149‑1 (MQ=255)       tcgctcgGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCt                                                                                                                                            >  2:53220/1‑149 (MQ=255)         gctcgGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑g                                                                                                                                         <  3:185333/149‑1 (MQ=255)                        ccTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCg                                                                                                                          >  8:91392/1‑149 (MQ=255)                            gACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGt                                                                                                                        <  2:97829/147‑1 (MQ=255)                            gACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTc                                                                                                                       <  7:91392/148‑1 (MQ=255)                              cGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGc                                                                                                                    >  3:245300/1‑149 (MQ=255)                                 aCGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCg                                                                                                                 <  4:230247/149‑1 (MQ=255)                                         gctTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTg                                                                                                         >  6:92590/1‑149 (MQ=255)                                          ctTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTgg                                                                                                        >  6:20552/1‑149 (MQ=255)                                           ttCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGt                                                                                                       >  1:265288/1‑149 (MQ=255)                                              gAAGGTACGCAGGTCGTCGCTGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGa                                                                                                    <  8:66826/149‑1 (MQ=255)                                                 ggTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAg                                                                                                  >  3:189432/1‑148 (MQ=255)                                                     cGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACtt                                                                                             <  3:245407/149‑1 (MQ=255)                                                      gCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTg                                                                                            <  6:195056/149‑1 (MQ=255)                                                      gCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTg                                                                                            >  2:176276/1‑149 (MQ=255)                                                      gCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGAGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTg                                                                                            >  1:106395/1‑149 (MQ=255)                                                       cAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTgg                                                                                           >  4:210937/1‑149 (MQ=255)                                                        aGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGc                                                                                          <  5:20552/149‑1 (MQ=255)                                                        aGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGc                                                                                          >  8:11633/1‑149 (MQ=255)                                                          gtcgtcGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCct                                                                                        <  2:265288/149‑1 (MQ=255)                                                             gtcgATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCctga                                                                                      <  7:23189/148‑1 (MQ=255)                                                             gtcgATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCctga                                                                                      <  4:245300/148‑1 (MQ=255)                                                                  tGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATg                                                                                <  1:176276/149‑1 (MQ=255)                                                                                         cGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGt                                                                            <  7:34239/130‑1 (MQ=255)                                                                                         cGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGt                                                                            >  8:34239/1‑130 (MQ=255)                                                                                           gATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCg                                                                    <  3:33574/136‑1 (MQ=255)                                                                                           gATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCg                                                                    >  4:33574/1‑136 (MQ=255)                                                                                             tCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCtt                                                                                                                                           >  3:4552/1‑64 (MQ=255)                                                                                             tCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCtt                                                                                                                                           <  4:4552/64‑1 (MQ=255)                                                                                                ggATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCAc                                                   >  6:118686/1‑148 (MQ=255)                                                                                                     ttGAACTGCTTGGCCAGGGCGAAGGTCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACca                                                 <  6:220356/145‑1 (MQ=255)                                                                                                     ttGAACTGCTTGGCCAGGGCGAAGGTCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACca                                                 >  5:220356/1‑145 (MQ=255)                                                                                                     ttGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACcagca                                              <  5:118686/148‑1 (MQ=255)                                                                                                      tGAACTGCTTGGCCACGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACcagcag                                             >  5:178426/1‑148 (MQ=255)                                                                                                       gAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAg                                                      <  8:51531/138‑1 (MQ=255)                                                                                                       gAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAg                                                      >  7:51531/1‑138 (MQ=255)                                                                                                       gAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAgcagca                                           >  1:248867/1‑149 (MQ=255)                                                                                                       gAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACACTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCagcagcc                                           >  6:6743/1‑148 (MQ=255)                                                                                                       gAACTGCTTGGCCAGGGCGAAGATCACCCCCCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAgcagca                                           >  1:23421/1‑149 (MQ=255)                                                                                                        aaCTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCAc                                          >  4:33290/1‑149 (MQ=255)                                                                                                             cTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTg                                                                                                     >  5:77639/1‑85 (MQ=255)                                                                                                             cTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTg                                                                                                     <  6:77639/85‑1 (MQ=255)                                                                                                             cTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCg                                                              >  1:56949/1‑124 (MQ=255)                                                                                                             cTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCg                                                              <  2:56949/124‑1 (MQ=255)                                                                                                                   cAGGGCGAAGATCACCCCGCCCTTGGCAGTACCGTCCAGCTTGGGACAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCCCCTCGTgcggc                                >  1:191450/1‑149 (MQ=255)                                                                                                                   cAGGGCGAAGATCACCCCGCCCTTGGCAGTACCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTCGTgcggcg                               >  1:48025/1‑149 (MQ=255)                                                                                                                       gCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTCGTGCGGCGCCt                            >  5:30879/1‑148 (MQ=255)                                                                                                                             aTCACCCCGCCCTTGGCAGTACCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTCGTGCGGCGCCTCAACGTc                     <  2:48025/149‑1 (MQ=255)                                                                                                                              tCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTCGTGCGGCGCCTCAACGTCg                    <  2:23421/149‑1 (MQ=255)                                                                                                                              tCACCCCGCCCTTGGCAGTACCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTCGTGCGGCGCCTCAACGTc                     <  2:191450/148‑1 (MQ=255)                                                                                                                                aCCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTCGTGCGGCGCCTCAACGTCGa                   <  3:33290/148‑1 (MQ=255)                                                                                                                                  cccGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGtt                                                                                                      <  3:16072/63‑1 (MQ=255)                                                                                                                                  cccGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGtt                                                                                                      >  4:16072/1‑63 (MQ=255)                                                                                                                                  cccGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTc                                       >  3:188715/1‑126 (MQ=255)                                                                                                                                  cccGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTc                                       <  4:188715/126‑1 (MQ=255)                                                                                                                                         ttGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTCGTGCGGCGCCTCAACGTCGAGCTTGc             <  7:61271/145‑1 (MQ=255)                                                                                                                                         ttGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTCGTGCGGCGCCTCAACGTCGAGCTTGc             >  8:61271/1‑145 (MQ=255)                                                                                                                                         ttGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTCGTGCGGCGCCTCAACGTCGAGCTTGCCGAt         >  4:3112/1‑149 (MQ=255)                                                                                                                                                tGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTCGTGCGGCGCCTCAACGTCGAGCTTGCCGATGACCCgg  <  1:53220/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     TCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTT‑GGTCAGGGCCAGGCCGGTCAGCTCGACGCTCTGGTTGAAGTACTTGGCCTGACTGATGGCGTTCTGGCCGGTGCCGGCGTCAAGCACCAGCAGCACCTCGTGCGGCGCCTCAACGTCGAGCTTGCCGATGACCCGG  >  NC_002947/5830659‑5830950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑