Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,841,904 C→T 10.8% A158V (GCG→GTG) ‡ calB → coniferyl aldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,841,9040CT10.8% 120.2 / 6.0 47A158V (GCG→GTG) ‡calBconiferyl aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (20/21);  new base T (2/3);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

CCAGCGCACAGGTGCGCTATCAACCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCTTCGACCACCTGCTGTTCACTGGTGCAACCAGCGTGGG  >  NC_002947/5841757‑5842048
                                                                                                                                                   |                                                                                                                                                
ataGCGCACCTGTGCGCTATCAACCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCcgc                                                                                                                                                  >  7:154824/3‑148 (MQ=255)
 cAGCGCACAGGTGCGCTATCAACCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGa                                                                                                                                                >  7:159801/1‑149 (MQ=255)
 cAGCGCACAGGTGCGCTATCAACCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGa                                                                                                                                                >  1:241694/1‑149 (MQ=255)
     gcACAGGTGCGCTATCAACCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACggg                                                                                                                                            <  8:241283/149‑1 (MQ=255)
             gcgcTATCAACCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTCGCGAGGCCACGCCCGAGACGGGCCTGGccc                                                                                                                                    >  8:163601/1‑149 (MQ=255)
              cgcTATCAACCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCAGGCCCGTCGCGGGCGTGGcctc                                                                                                                                   <  4:50324/149‑3 (MQ=255)
               gcTATCAACCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTg                                                                                                                                  <  4:63786/149‑1 (MQ=255)
               gcTATCAACCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTg                                                                                                                                  >  2:205044/1‑149 (MQ=255)
                   tCAACCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATACTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTgcag                                                                                                                               >  3:181462/1‑148 (MQ=255)
                     aaCCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCAGGCCCGTCGCGGGCGTGGcctcgc                                                                                                                                 >  3:24574/1‑140 (MQ=255)
                     aaCCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCAGGCCCGTCGCGGGCGTGGcctcgc                                                                                                                                 <  4:24574/144‑5 (MQ=255)
                     aaCCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCaa                                                                                                                            >  5:48547/1‑149 (MQ=255)
                               cGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGc                                                                                                                  <  2:74219/149‑1 (MQ=255)
                                 tGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCgt                                                                                                                <  2:241694/149‑1 (MQ=255)
                                             tCGTGCCGTGGTAATACCCGTTGTTACTCGGCCTCGGCCCGGTGTCCTTCGGCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCcaca                                                                                                    <  2:168967/149‑1 (MQ=255)
                                                         aCTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGc                                                                                        >  7:171015/1‑149 (MQ=255)
                                                            aCCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGtt                                                                                     >  4:194692/1‑149 (MQ=255)
                                                             cccGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGtt                                                                                     <  8:117180/148‑1 (MQ=255)
                                                             cccGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTtg                                                                                    <  1:205044/149‑1 (MQ=255)
                                                               cGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTtgtg                                                                                  >  2:205593/1‑149 (MQ=255)
                                                                          gCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAg                                                                         <  2:93727/147‑1 (MQ=255)
                                                                                ggCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGtt                                                                                     <  3:52165/129‑1 (MQ=255)
                                                                                ggCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGtt                                                                                     >  4:52165/1‑129 (MQ=255)
                                                                                        gACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAg                                                          <  7:20372/148‑1 (MQ=255)
                                                                                           cTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCt                                                      >  2:130957/1‑149 (MQ=255)
                                                                                             gcgcCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTgg                                                                                             <  2:34673/108‑1 (MQ=255)
                                                                                              cgcCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTcg                                                   >  2:202344/1‑149 (MQ=255)
                                                                                                cccTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTc                                                                               >  7:207314/1‑119 (MQ=255)
                                                                                                cccTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTc                                                                               <  8:207314/119‑1 (MQ=255)
                                                                                                     gccgccGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTcgcccgc                                              <  6:48547/147‑1 (MQ=255)
                                                                                                      ccgccgGCAACCGGGTCATTCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTg                                           <  4:181462/149‑1 (MQ=255)
                                                                                                       cgccgGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGc                                          <  8:154824/149‑1 (MQ=255)
                                                                                                           ggCAACCGGGTCATGGTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTTTGTTCTCCACAGACTTGGTCAGCGTTGTGCTTGGTGAGGTCGAGGTGGGCTAGGCCTTcgc                                                  <  8:39685/137‑1 (MQ=255)
                                                                                                           ggCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCtt                                      <  8:171015/149‑1 (MQ=255)
                                                                                                           ggCAACCGGGTCATGCTCAAGCTTAGCGAGACCACGCCCGCTACAGGACTGGCCCTGCAGCAACTGATCGAGCGTATGTTCACCACAGACCTGGTCAGCGTTGTGCTAGGTGAGGTCGAGGTGGGCCAGGCCTTcgc                                                  >  7:39685/1‑137 (MQ=255)
                                                                                                            gCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCTTc                                     >  7:33307/1‑149 (MQ=255)
                                                                                                                   ggTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCTTCGaccacc                              >  1:257840/1‑149 (MQ=255)
                                                                                                                           tCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCTTCGACCACCTGCTGtt                       >  7:178571/1‑148 (MQ=255)
                                                                                                                             aaGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTc                                                    >  6:277564/1‑117 (MQ=255)
                                                                                                                             aaGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTc                                                    <  5:277564/117‑1 (MQ=255)
                                                                                                                             aaGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCTTCGACCACCTGCTGTTCa                     <  3:194692/148‑1 (MQ=255)
                                                                                                                             aaGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCTTCGACCACCTGCTGTTCAc                    >  1:91973/1‑149 (MQ=255)
                                                                                                                                   aGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCTTCGACCACCTGCTGTTCACTGGt                <  8:178571/147‑1 (MQ=255)
                                                                                                                                      agggCCAGGCCCGTCGCGGGCGTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCTTCGACCACCTGCTGTTc                      >  6:61113/3‑138 (MQ=255)
                                                                                                                                      agggCCAGGCCCGTCGCGGGCGTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCTTCGACCACCTGCTGTTc                      <  5:61113/136‑1 (MQ=255)
                                                                                                                                            aCGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCTTCGACCACCTGCTGTTCACTGGTGCAACCAGCGt     >  8:241982/1‑149 (MQ=255)
                                                                                                                                              gCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTCGGCCAGGCCTTCGCCCGCCTGCCCTTCGACCACCTGCTGTTCACTGGTGCACACAGCGTgg   >  7:117106/1‑149 (MQ=255)
                                                                                                                                               cccGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCTTCGACCACCTGCTGTTCACTGGTGCAACCAGCGTggg  <  1:202344/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCAGCGCACAGGTGCGCTATCAACCCTTGGGCGTGGTCGGCGTCATCGTGCCGTGGAACTACCCGTTGTTCCTCGCCATCGGCCCGTTGACCTGCGCCCTGGCCGCCGGCAACCGGGTCATGCTCAAGCTTAGCGAGGCCACGCCCGCGACGGGCCTGGCCCTGCAGCAACTGCTCGAGCGTGTGTTCCCCACAGACCTGGTCAGCGTTGTGCTCGGTGAGGTCGAGGTGGGCCAGGCCTTCGCCCGCCTGCCCTTCGACCACCTGCTGTTCACTGGTGCAACCAGCGTGGG  >  NC_002947/5841757‑5842048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑