Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,849,048 A→G 5.8% intergenic (+23/‑248) rlmI → / → ilvD ribosomal RNA large subunit methyltransferase I/dihydroxy‑acid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,849,0480AG 5.8% 206.6 / 2.1 69intergenic (+23/‑248)rlmI/ilvDribosomal RNA large subunit methyltransferase I/dihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base A (34/31);  new base G (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

CCTGGACCGCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATCCCGCGC  >  NC_002947/5848902‑5849192
                                                                                                                                                  |                                                                                                                                                
ccTGGACCGCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGc                                                                                                                                                <  8:73576/149‑1 (MQ=255)
      ccGCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCt                                                                                                                                          >  3:210838/1‑149 (MQ=255)
            cATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCTGGGCGGCCCtttttgg                                                                                                                                    <  8:98857/149‑5 (MQ=255)
              tGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGcc                                                                                                                                  <  4:210838/149‑1 (MQ=255)
              tGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGcc                                                                                                                                  <  8:48599/149‑1 (MQ=255)
                   ctgctCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAAt                                                                                                                             <  8:155314/149‑1 (MQ=255)
                     gctCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATcc                                                                                                                           >  5:219516/1‑149 (MQ=255)
                         gagcgagGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATcccccc                                                                                                                       <  6:219516/149‑1 (MQ=255)
                             gagGCGGTCAGGGCCAGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCATGTTACTGACAAACAGCTGATAAATGACAAAAAAGGCCGCACAGCGGCCCTTTCCTGCCGCAATACCCCCGca                                                                                                                    >  4:173516/1‑147 (MQ=255)
                              tggCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCaa                                                                                                                  <  5:197692/148‑1 (MQ=255)
                              agGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAACCCCCCCGCCaa                                                                                                                  <  3:16358/149‑1 (MQ=255)
                               ggCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAAc                                                                                                                 <  8:158349/149‑1 (MQ=255)
                                gCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCAATCCTGCCGCAAACCCCCCGCCAAca                                                                                                                >  6:197692/1‑148 (MQ=255)
                                   gTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATc                                                                                                             <  6:198437/149‑1 (MQ=255)
                                         gCCCGGATCACCCGGTACACCCGGCCATCGCCGAACCCCGATACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATc                                                                                                       >  1:246636/1‑149 (MQ=255)
                                           ccGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTg                                                                                                     >  6:229258/1‑149 (MQ=255)
                                                    ccGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCg                                                                                                                                               >  8:33741/1‑98 (MQ=255)
                                                    ccGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCg                                                                                                                                               <  7:33741/98‑1 (MQ=255)
                                                    ccGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCt                                                                                             >  3:206324/1‑148 (MQ=255)
                                                      ggTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCACTCCCCCCGCCAACCATCCACATCTGCATTCCCTCAc                                                                                          <  6:134919/149‑1 (MQ=255)
                                                             ccGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACcgctggc                                                                                   >  3:212358/1‑145 (MQ=255)
                                                             ccGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACAGATGCATTCCCTCACCgcgc                                                                                     >  3:11142/1‑147 (MQ=255)
                                                                   aTCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGgtg                                                                             >  6:125017/1‑149 (MQ=255)
                                                                         gAAAACCGCTACATCAAAAGCATCACCTGCAGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGGCAGCGGTGTAGAAt                                                                       <  8:24154/149‑1 (MQ=255)
                                                                            aCCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCgg                                                                    <  5:125017/149‑1 (MQ=255)
                                                                                gCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTa                                                                >  4:58524/1‑149 (MQ=255)
                                                                                     aTCAAAAGCATCACCTGCAGGTTACTGCCAAACAGCTGATTTCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCAt                                                           <  7:162658/149‑1 (MQ=255)
                                                                                      tCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATc                                                          >  2:149605/1‑149 (MQ=255)
                                                                                       cAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCAATCCCTCACCGCCCCAGCGGTGTAGAATCGGCCAATTCATCg                                                         >  1:260729/1‑149 (MQ=255)
                                                                                        aaaaGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGCGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGc                                                        >  2:136954/1‑149 (MQ=255)
                                                                                         aaaGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCTGTGTAGAATCGGCCTATTCATCGcc                                                       <  5:229258/149‑1 (MQ=255)
                                                                                          aaGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCAAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCa                                                      <  4:167844/149‑1 (MQ=255)
                                                                                           aGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCACCCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAg                                                     <  1:136954/149‑1 (MQ=255)
                                                                                             cATCGCCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGt                                                    >  4:168966/1‑148 (MQ=255)
                                                                                               tCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCAt                                                 >  5:236128/1‑149 (MQ=255)
                                                                                               tCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGAAGCAATCCCCCCGCCAACCATCCACATCTGCATTACCTCACAGCGCCAGCGGTGTAGAATCGGCATATTCATCGCCAGTCAt                                                 >  4:148451/1‑149 (MQ=255)
                                                                                                cACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATc                                                >  5:76208/1‑149 (MQ=255)
                                                                                                   cTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATcccc                                             >  2:206457/1‑149 (MQ=255)
                                                                                                      ccGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCcggc                                          <  6:236128/149‑1 (MQ=255)
                                                                                                      ccGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCcggc                                          >  4:56774/1‑149 (MQ=255)
                                                                                                          ttACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGt                                      <  3:56774/149‑1 (MQ=255)
                                                                                                                ccAAACAGCTGATATCTGCCAAAACCGGCCGCCCATCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTAt                                  <  3:148451/147‑1 (MQ=255)
                                                                                                                ccAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTAt                                  <  5:28193/147‑1 (MQ=255)
                                                                                                                caaaaCAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACAGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTAt                                  >  2:103164/3‑147 (MQ=255)
                                                                                                                  aaaCAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGc                              >  3:133937/1‑149 (MQ=255)
                                                                                                                     cAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTAt                                  <  4:98842/142‑1 (MQ=255)
                                                                                                                     cAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTAt                                  >  3:98842/1‑142 (MQ=255)
                                                                                                                        cTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGt                        >  2:27748/1‑149 (MQ=255)
                                                                                                                        cTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGt                        >  2:46790/1‑149 (MQ=255)
                                                                                                                         tGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGt                        >  6:248548/1‑148 (MQ=255)
                                                                                                                           atatCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTa                                                                <  4:177697/106‑1 (MQ=255)
                                                                                                                           atatCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTa                                                                >  3:177697/1‑106 (MQ=255)
                                                                                                                           atatCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCaa                     <  6:231774/149‑1 (MQ=255)
                                                                                                                                tGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCCCGCCAGCGGTGTAGAATCGGCCTATTCATCCCCAGTCATCCCCGGCGGGTATATGAGCTCTGGTAAAGCAcg                >  4:240910/1‑149 (MQ=255)
                                                                                                                                 gCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAg                                                     <  5:103061/111‑1 (MQ=255)
                                                                                                                                 gCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAg                                                     >  6:103061/1‑111 (MQ=255)
                                                                                                                                  ccAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTAt                                  >  2:268767/1‑129 (MQ=255)
                                                                                                                                  ccAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTAt                                  <  1:268767/129‑1 (MQ=255)
                                                                                                                                  ccAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCAcgcg              >  7:118337/1‑149 (MQ=255)
                                                                                                                                  ccAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCAcgcg              <  8:8808/149‑1 (MQ=255)
                                                                                                                                   cAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGACAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACgcgg             <  3:240910/149‑1 (MQ=255)
                                                                                                                                   aggaaaGGGCCGCTGGGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGc                                                        >  3:29358/4‑106 (MQ=255)
                                                                                                                                   aggaaaGGGCCGCTGGGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGc                                                        <  4:29358/103‑1 (MQ=255)
                                                                                                                                     gaaaGGGCCGCTGGGCGGCCCTTTCCTGCCCCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACgcggc            >  1:23578/2‑148 (MQ=255)
                                                                                                                                     aaaaGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACgcggc            >  8:99098/1‑148 (MQ=255)
                                                                                                                                       aaGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGAt         <  8:118337/149‑1 (MQ=255)
                                                                                                                                         gggCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATcc       >  5:163018/1‑149 (MQ=255)
                                                                                                                                             cgccCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATCCcgcg   <  2:246636/149‑1 (MQ=255)
                                                                                                                                              gccCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTTGTCAAGCACGCGGCGATCCcgcgc  <  1:103164/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCTGGACCGCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTCAAGCACGCGGCGATCCCGCGC  >  NC_002947/5848902‑5849192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑