Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,859,607 C→T 9.3% A127T (GCC→ACC)  PP_5135 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,859,6070CT 9.3% 117.8 / 3.0 43A127T (GCC→ACC) PP_5135ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (21/18);  new base T (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

CTGACATCCAGGAAGCCGGTGCCGGACGAGGCCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGAGGATGGCCAGGCTCGATGCCGCCAGCCCCCACACAGC  >  NC_002947/5859459‑5859754
                                                                                                                                                    |                                                                                                                                                   
cTGACATCCAGGAAGCCGGTGCCGGACGAGGCCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTggcggc                                                                                                                                                     >  7:187717/1‑149 (MQ=255)
 tGACATCCAGGAAGCCGGTGCCGGACGAGGCCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGcc                                                                                                                                                    >  1:161886/1‑149 (MQ=255)
   aCATCCAGGAAGCCGGTGCCGGACGAGGCCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGcc                                                                                                                                                    <  2:161886/147‑1 (MQ=255)
   aCATCCAGGAAGCCGGTGCCGGACGAGGCCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGATGGCGGccc                                                                                                                                                   <  8:196612/148‑1 (MQ=255)
           gAAGCCGGTGCCGGACGAGGCCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCa                                                                                                                                          >  5:95053/1‑149 (MQ=255)
           gAAGCCGGTGCCGGACGAGGCCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCa                                                                                                                                          >  2:69138/1‑149 (MQ=255)
               ccGGTGCCGGACGAGGCCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGcc                                                                                                                                      >  8:223300/1‑149 (MQ=255)
                 ggTTCCGGACGAGGCCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTTGTCAGGTCCTGCCAGCTCACCGGTTTTCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGCGGCGGCCCACACGTCCATGCCGa                                                                                                                                    <  7:162264/149‑1 (MQ=255)
                     ccGGACGAGGGCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCa                                                                                                                                <  8:99899/149‑1 (MQ=255)
                      cGGACGAGGCCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAg                                                                                                                               >  3:173074/1‑149 (MQ=255)
                              gCCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACGGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCgg                                                                                                                       <  2:109024/149‑1 (MQ=255)
                                  ggTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTg                                                                                                                   >  5:248543/1‑149 (MQ=255)
                                   gTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTgg                                                                                                                  <  8:187717/149‑1 (MQ=255)
                                                   ttGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAAtt                                                                                                  >  6:97275/1‑149 (MQ=255)
                                                        tttGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCg                                                                                              >  3:151374/1‑148 (MQ=255)
                                                          tGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCgg                                                                                             <  7:223300/147‑1 (MQ=255)
                                                          tGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCg                                                                                              <  4:151374/146‑1 (MQ=255)
                                                            tACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGAt                                                                                         <  1:69138/149‑1 (MQ=255)
                                                            tACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGAt                                                                                         <  4:173074/149‑1 (MQ=255)
                                                               tCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCtt                                                                                      <  6:248543/149‑1 (MQ=255)
                                                                   gCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGccc                                                                                  <  1:109283/149‑1 (MQ=255)
                                                                              tCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAATCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTAGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTgg                                                                       <  1:190094/149‑1 (MQ=255)
                                                                                   ccAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGATGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCa                                                                   >  1:81935/1‑148 (MQ=255)
                                                                                        tCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCt                                                             >  4:45859/1‑149 (MQ=255)
                                                                                          aCCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTc                                                           <  5:259520/149‑1 (MQ=255)
                                                                                                        aCCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAg                                                                                >  8:55376/1‑114 (MQ=255)
                                                                                                        aCCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAg                                                                                <  7:55376/114‑1 (MQ=255)
                                                                                                               ttCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACAAGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGa                                       <  2:81935/148‑1 (MQ=255)
                                                                                                                 ctcGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGa                                       >  3:63677/1‑146 (MQ=255)
                                                                                                                 ctcGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGa                                       <  4:63677/146‑1 (MQ=255)
                                                                                                                 ctcGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGAGGa                                    >  5:16057/1‑149 (MQ=255)
                                                                                                                  tcGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGAGGAt                                   >  5:157390/1‑149 (MQ=255)
                                                                                                                  tcGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGAGGAt                                   >  3:240274/1‑149 (MQ=255)
                                                                                                                         cGATGGTGTTGAAGCAAATGGTGGCGGTCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCAt                                                     <  2:268966/124‑1 (MQ=255)
                                                                                                                         cGATGGTGTTGAAGCAAATGGTGGCGGTCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCAt                                                     >  5:155477/1‑124 (MQ=255)
                                                                                                                         cGATGGTGTTGAAGCAAATGGTGGCGGTCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCAt                                                     >  1:268966/1‑124 (MQ=255)
                                                                                                                         cGATGGTGTTGAAGCAAATGGTGGCGGTCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCAt                                                     <  6:155477/124‑1 (MQ=255)
                                                                                                                            tGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGAGGATGGCCAGGCTc                         >  6:142923/1‑149 (MQ=255)
                                                                                                                                 ttGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATa                                                                 >  7:22641/1‑104 (MQ=255)
                                                                                                                                 ttGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATa                                                                 <  8:22641/104‑1 (MQ=255)
                                                                                                                                      gCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGAGGATGGCCAGGCTCGATgccgc                 >  4:140064/1‑147 (MQ=255)
                                                                                                                                        aaaTGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGc                                                                                                <  3:5929/66‑1 (MQ=255)
                                                                                                                                        aaaTGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGc                                                                                                >  4:5929/1‑66 (MQ=255)
                                                                                                                                        aaaTGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGAGGATGGCCAGGCTCGATGCCGCCAGc             >  7:194354/1‑149 (MQ=255)
                                                                                                                                         aaTGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGAGGATGGCCAGGCTCGATGCCGCCAGcc            <  6:157390/149‑1 (MQ=255)
                                                                                                                                           tggtggCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGAGGATGGCCAGGCTCGATGCCGCCAGcccc          >  8:134670/1‑149 (MQ=255)
                                                                                                                                             gtggCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCCTGCCGTTCTGGTCGAGGATGGCCAGGCTCGATGCCGCCAGCCCCca        >  5:137180/1‑149 (MQ=255)
                                                                                                                                                   gcCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGAGGATGGCCAGGCTCGATGCCGCCAGCCCCCACACAGc  >  2:38514/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTGACATCCAGGAAGCCGGTGCCGGACGAGGCCGGGTTGGGCATGACGATCTTGCCTTTGTACTCAGGCTTGGTCAGGTCCTGCCAGCTCACCGGTTTGCTCAGACCCTGTTTCTCGGCCTCGATGGTGTTGAAGCAAATGGTGGCGGCCCACACGTCCATGCCGACCCAGGCTGGCGGGTTGGCGGCGTCGCGATAATTGCCGGAGATCTTGCCCAGGTCCTTTGGCGCATAGGCTTCGAGCATGCCGTTCTGGTCGAGGATGGCCAGGCTCGATGCCGCCAGCCCCCACACAGC  >  NC_002947/5859459‑5859754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑