Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,869,612	T→C	7.1%	P40P (CCT→CCC)	PP_5144 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,869,612	0	T	C	7.1%	163.4 / 2.6	56	P40P (CCT→CCC)	PP_5144	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/24);  new base C (2/2);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01

ACCAAAAAACACAGAGGCCCAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCTGGTGGCAGCGTTTCT  >  NC_002947/5869468‑5869758                                                                                                                                                  |                                                                                                                                                   aCCAAAAAACACAGAGGCCCAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTggg                                                                                                                                                 <  2:266939/148‑1 (MQ=255)   cAAAAAACACAGAGGCCCAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTg                                                                                                                                              <  4:116498/149‑1 (MQ=255)       aaaCACAGAGGCCCAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCCCAGGTGCAtc                                                                                                                                          <  4:203422/149‑2 (MQ=255)         acacAGAGGCCCAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGcc                                                                                                                                        <  5:153230/149‑1 (MQ=255)           acaGAGGCCCAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCa                                                                                                                                            <  8:46043/143‑1 (MQ=255)           acaGAGGCCCAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCa                                                                                                                                            >  7:46043/1‑143 (MQ=255)           acaGAGGCCCAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCCCAGGTGCAtcctcc                                                                                                                                      <  1:244320/149‑6 (MQ=255)            caGAGGCCCAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCggg                                                                                                                                     >  1:216608/1‑149 (MQ=255)              gagGCCCAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGgcg                                                                                                                                   <  7:109229/149‑1 (MQ=255)                  cccAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGAc                                                                                                                               <  3:168541/149‑1 (MQ=255)                   ccAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGAcc                                                                                                                              >  5:68956/1‑149 (MQ=255)                        cTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGAc                                                                                                                               <  8:212603/143‑1 (MQ=255)                        cTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGAc                                                                                                                               >  7:212603/1‑143 (MQ=255)                               ccTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCg                                                                                                                  <  5:104255/149‑1 (MQ=255)                                   aTCGTATTTGCGTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCGGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTa                                                                                                               <  2:66453/148‑1 (MQ=255)                                        aTTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACctggc                                                                                                         >  1:145855/1‑149 (MQ=255)                                            gCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTggg                                                                                                     >  3:35184/1‑149 (MQ=255)                                             ccTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTggg                                                                                                     <  2:216608/148‑1 (MQ=255)                                              cTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGAcc                                                                                                                              >  4:119888/1‑122 (MQ=255)                                                     ccGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GTATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGccc                                                                                            <  6:118201/149‑1 (MQ=255)                                                      cGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCca                                                                                           >  2:227836/1‑149 (MQ=255)                                                                      gCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCcg                                                                           >  2:249660/1‑149 (MQ=255)                                                                         gATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCgcgc                                                                        >  4:47285/1‑149 (MQ=255)                                                                          aTCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCt                                                                       >  2:240653/1‑149 (MQ=255)                                                                              tGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGAcc                                                                   <  4:35184/149‑1 (MQ=255)                                                                                   gcCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACAt                                                              <  1:227836/149‑1 (MQ=255)                                                                                    ccAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATc                                                             >  8:105336/1‑149 (MQ=255)                                                                                    ccAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATc                                                             <  7:105336/149‑1 (MQ=255)                                                                                             acgaATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCg                                                    >  8:127907/1‑149 (MQ=255)                                                                                               gaATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCggg                                                  >  3:203662/1‑149 (MQ=255)                                                                                                     tATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGAGGGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAgg                                             >  1:48146/1‑149 (MQ=255)                                                                                                         cccgcccCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGTGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAg                                        >  6:159327/1‑149 (MQ=255)                                                                                                         cccgcccCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAg                                        >  5:1461/1‑149 (MQ=255)                                                                                                          ccgcccCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAgg                                       >  8:184812/1‑149 (MQ=255)                                                                                                                cACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCctc                                 <  4:203662/149‑1 (MQ=255)                                                                                                                     aGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCctcgctc                             <  2:48146/148‑1 (MQ=255)                                                                                                                       ccccTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTcgcg                          >  8:242583/1‑149 (MQ=255)                                                                                                                        cccTGGCGGCGTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCggg                                                  <  7:217442/124‑1 (MQ=255)                                                                                                                        cccTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCTCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCgg                         >  4:58963/1‑149 (MQ=255)                                                                                                                        cccTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCggg                                                  >  8:217442/1‑124 (MQ=255)                                                                                                                         ccTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCctc                                 <  4:133451/140‑1 (MQ=255)                                                                                                                         ccTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCctc                                 >  3:133451/1‑140 (MQ=255)                                                                                                                             gcggcCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGa                    <  3:47285/149‑1 (MQ=255)                                                                                                                                gcCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCt                 <  1:240653/149‑1 (MQ=255)                                                                                                                                   tGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCtggt              >  2:41797/1‑149 (MQ=255)                                                                                                                                     gga‑ggaTGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCtggtgg            <  1:41797/149‑1 (MQ=255)                                                                                                                                      ga‑ggaTGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCctc                                 <  6:113054/127‑1 (MQ=255)                                                                                                                                      ga‑ggaTGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCctc                                 >  5:113054/1‑127 (MQ=255)                                                                                                                                      ccggcaTGCACCCCAGGTGCATCCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGTGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCtggtgg            >  2:11243/6‑149 (MQ=255)                                                                                                                                         gcaTGCACCCCAGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCTGGTGGCAg         >  8:52338/3‑149 (MQ=255)                                                                                                                                          gaTGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCTGGTGGCAGc        >  7:43423/1‑149 (MQ=255)                                                                                                                                               aCCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCTGGTGGCAGCGttt    >  1:91558/1‑148 (MQ=255)                                                                                                                                               aCCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCTGGTGGCAGCGttt    <  2:91558/148‑1 (MQ=255)                                                                                                                                               aCCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCTGGTGGCAGCGTTTc   >  8:40235/1‑149 (MQ=255)                                                                                                                                               aCCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCTGCTGGCAGCGTTTc   >  2:141329/1‑149 (MQ=255)                                                                                                                                                ccTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCTGGTGGCAGCGTTTCt  <  7:242583/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   ACCAAAAAACACAGAGGCCCAACCTATGTGCCTGATCGTATTTGCCTGGCGGCCGGGGCACGCCCTGCCGCTGATCGTGGCGGCCAACCGCGACGAATTCTATGCCCGCCCCACTCAGCCCCTGGCGGCCTGGGA‑GGATGCACCTGGGGTGCATGCCGGGCGCGACCTGGAGGCAGGCGGTACCTGGCTGGGCGTAGGCCCACAGGGGCGCTTTGCCGCGCTGACCAACATCCGCGACCCGGGCCAGGCGCAGGGGCCTCGCTCGCGGGGTGAGCTGGTGGCAGCGTTTCT  >  NC_002947/5869468‑5869758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑