Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,871,629 G→C 7.3% L342V (CTC→GTC)  ptsP ← phosphoenolpyruvate‑dependent regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,871,6290GC 7.3% 176.5 / 3.7 55L342V (CTC→GTC) ptsPphosphoenolpyruvate‑dependent regulator
Reads supporting (aligned to +/- strand):  ref base G (23/28);  new base C (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

GTCGACCTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAGTCGTCGTCC  >  NC_002947/5871489‑5871774
                                                                                                                                            |                                                                                                                                                 
gTCGACCTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCg                                                                                                                                           <  4:93882/149‑1 (MQ=255)
   gACCTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGGCCATGCTCGgtg                                                                                                                                        <  7:56386/149‑1 (MQ=255)
      cTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGgtgt                                                                                                                                       >  7:204687/1‑147 (MQ=255)
      cTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGgtgt                                                                                                                                       >  5:245257/1‑147 (MQ=255)
             tATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTc                                                                                                                              <  2:215222/149‑1 (MQ=255)
               tGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCa                                                                                                                             <  2:4996/148‑1 (MQ=255)
                        gACCAGGCCCATCACCGTGGGGATGCCCATGGCCCTCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGGCCATGCTCGgtgaag                                                                                                                                     <  5:28850/131‑4 (MQ=255)
                        gACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGGCCATGCTCGgtgaag                                                                                                                                     >  6:28850/1‑128 (MQ=255)
                           cAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATgg                                                                                                                >  8:251307/1‑149 (MQ=255)
                                   tCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCg                                                                                                        <  6:28704/149‑1 (MQ=255)
                                   tCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCg                                                                                                        <  8:204687/149‑1 (MQ=255)
                                       cGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGAGTTTGGAGTTGCCCGAGCGGAGTAGCGAGACCAGGCCGACCTGCTTGCCTTCCGGGACTTCACCGAGCATGGGCGGTGTCTGTTCCTGACTGAGCAGGATGGTGTTGTCggga                                                                                                     <  6:91381/148‑3 (MQ=255)
                                            ggATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATacac                                                                                                >  5:206845/1‑148 (MQ=255)
                                             gATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATg                                                                                                                 >  4:165533/1‑130 (MQ=255)
                                             gATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATg                                                                                                                 <  3:165533/130‑1 (MQ=255)
                                             gATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCa                                                                                              <  1:125890/149‑1 (MQ=255)
                                             gATGCCCATGGCCCGCACGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCTCCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCa                                                                                              <  8:1526/149‑1 (MQ=255)
                                                 cccATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGa                                                                                          <  8:200669/149‑1 (MQ=255)
                                                  ccATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGAc                                                                                         >  1:269846/1‑149 (MQ=255)
                                                         ccGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAgc                                                                                  >  6:33823/1‑149 (MQ=255)
                                                                   aTGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTg                                                                        <  5:33823/149‑1 (MQ=255)
                                                                      gCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAg                                                                     >  5:106040/1‑149 (MQ=255)
                                                                       cGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAgg                                                                    >  6:239914/1‑149 (MQ=255)
                                                                       cGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAgg                                                                    >  7:44513/1‑149 (MQ=255)
                                                                                      ccgagccgagTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAggcgg                                                     >  8:29518/1‑149 (MQ=255)
                                                                                       cgagccgagTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACAACAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAggcggc                                                    >  6:256769/1‑149 (MQ=255)
                                                                                           ccgagTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGccc                                                <  7:29518/149‑1 (MQ=255)
                                                                                            cgagTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGcccc                                               <  5:239914/149‑1 (MQ=255)
                                                                                            cgagTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGccc                                                >  4:68049/1‑148 (MQ=255)
                                                                                              agTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAg                                             <  7:251307/149‑1 (MQ=255)
                                                                                              agTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCC‑AAAGGCGGCGCCCCAgg                                            <  6:106040/149‑1 (MQ=255)
                                                                                                tACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGt                                           >  6:4916/1‑149 (MQ=255)
                                                                                                        ccAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACg                                   >  3:140063/1‑149 (MQ=255)
                                                                                                         cAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGt                                  >  1:258552/1‑149 (MQ=255)
                                                                                                            gCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCgg                               <  2:206001/149‑1 (MQ=255)
                                                                                                            gCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCg                                >  4:220624/1‑148 (MQ=255)
                                                                                                             ccGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGa                              <  3:220624/149‑1 (MQ=255)
                                                                                                              cGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTcc                                         <  2:22208/137‑1 (MQ=255)
                                                                                                              cGACCAGCTTGCCTTCCGGCACTTCACCGACCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTcc                                         >  1:22208/1‑137 (MQ=255)
                                                                                                                   aGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACg                        <  5:256769/149‑1 (MQ=255)
                                                                                                                    gCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGc                       >  2:7153/1‑149 (MQ=255)
                                                                                                                    gCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGc                       <  2:269846/149‑1 (MQ=255)
                                                                                                                      ttGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCt                      >  5:225398/1‑148 (MQ=255)
                                                                                                                         ccTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCAc                   <  3:170558/148‑1 (MQ=255)
                                                                                                                         ccTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACg                  <  4:140063/149‑1 (MQ=255)
                                                                                                                                gCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACAGACCAGGATGGTGTTGTCGGCATACACCACCGACTGCGAGCGCGATTACTGCAGGTAGGCCAAAAGGCGGCGCCCCAGCTCCTACACGTCGGAGGCACGCACACCCAggaa            >  2:182220/1‑146 (MQ=255)
                                                                                                                                      cACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCAGGCTCACGCAGGTAgtcgtc      <  6:206845/148‑1 (MQ=255)
                                                                                                                                       aCCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgtcg     <  1:85032/148‑1 (MQ=255)
                                                                                                                                        ccGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCa                                                                                                     <  2:229374/51‑1 (MQ=255)
                                                                                                                                        ccGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCa                                                                                                     >  1:229374/1‑51 (MQ=255)
                                                                                                                                        ccGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgtcgtc   <  2:258552/149‑1 (MQ=255)
                                                                                                                                         cGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAgtcgtcgtc   >  4:241112/1‑148 (MQ=255)
                                                                                                                                         cGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAGTCATCGTcc  <  1:7153/149‑1 (MQ=255)
                                                                                                                                          gAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGa                                                                                          >  6:115825/1‑60 (MQ=255)
                                                                                                                                          gAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGa                                                                                          <  5:115825/60‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
GTCGACCTTGGAGTATGGCAGGTCGACCAGGCCCATCACCGTGGGGATGCCCATGGCCCGCGCGAGGATGGCGACATGGGAGTTGCCCGAGCCGAGTACCGAGACCAGGCCGACCAGCTTGCCTTCCGGCACTTCACCGAGCATGGCCGGTGTCAGTTCCTCACTGACCAGGATGGTGTTGTCGGCATACACCAGCGACTGCGAGCGCGCTTCCTGCAGGTAGGCCAAAAGGCGGCGCCCCAGGTCCTTGACGTCGGAGGCACGCTCACGCAGGTAGTCGTCGTCC  >  NC_002947/5871489‑5871774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑