Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,900,951 A→G 8.7% V334A (GTG→GCG)  PP_5174 ← RND family transporter membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,900,9510AG 8.7% 160.7 / 4.9 57V334A (GTG→GCG) PP_5174RND family transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base A (27/25);  new base G (3/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

CCATCAACAGGCTAACGAACATCAGGTACCAGACGAACGATTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAGGCA  >  NC_002947/5900803‑5901094
                                                                                                                                                    |                                                                                                                                               
ccATCAACAGGCTAACGAACATCAGGTACCAGACGAACGATTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCa                                                                                                                                                 >  7:84690/1‑149 (MQ=255)
  aTCAACAGGCTAACGAACATCAGGTACCAGACGAACGATTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCAcg                                                                                                                                               >  5:60238/1‑149 (MQ=255)
           cTAACGAACATCAGGTACCAGACGAACGATTGTTGCTGGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGAACACGCGCTCACCg                                                                                                                                      <  4:153249/149‑1 (MQ=255)
            tAACGAACATCAGGTACCAGACGAACGATTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCgg                                                                                                                                     >  3:257732/1‑149 (MQ=255)
                                 cgaacgaTTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCa                                                                                                                <  3:159102/149‑1 (MQ=255)
                                  gaacgaTTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGc                                                                                                                                   <  3:46476/129‑1 (MQ=255)
                                   aacgaTTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCAc                                                                                                               >  3:79679/1‑148 (MQ=255)
                                        ttGATGCTTGAGTGCCCAGTCGGACAGGTTTAAGCTTCCTTTCATTGCTCATCCTAGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGACGGCGGTGACCACGCGTGCACCGGGCTGCACACCGGCGATCAGCACGATGCt                                                                                                         <  1:40627/149‑1 (MQ=255)
                                               ttGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCGTTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCgg                                                                                                                         <  5:223762/126‑1 (MQ=255)
                                               ttGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCgg                                                                                                                         >  6:223762/1‑126 (MQ=255)
                                                 gAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAgcg                                                                                                  <  6:60238/147‑1 (MQ=255)
                                                   gTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAgcggtgc                                                                                              <  3:64927/149‑1 (MQ=255)
                                                       ccAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTc                                                                                          >  7:62800/1‑149 (MQ=255)
                                                        cAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCACTCAGCACGATGCTGTCAGCGGTGCGGTCg                                                                                         <  2:130519/149‑1 (MQ=255)
                                                             ggACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCCTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAg                                                                                    >  6:199803/1‑149 (MQ=255)
                                                                   gTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCg                                                                                                                          >  7:32698/1‑105 (MQ=255)
                                                                   gTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCg                                                                                                                          <  8:32698/105‑1 (MQ=255)
                                                                    ttGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAgcggtgcggt                                                                                           >  7:7510/1‑135 (MQ=255)
                                                                    ttGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAgcggtgcggt                                                                                           <  8:7510/135‑1 (MQ=255)
                                                                        aGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCAc                                                                         <  8:84690/149‑1 (MQ=255)
                                                                                   aTTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAgcggt                                                                                                >  1:222547/1‑115 (MQ=255)
                                                                                   aTTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAgcggt                                                                                                <  2:222547/115‑1 (MQ=255)
                                                                                      gcgcATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGt                                                            <  4:79679/148‑1 (MQ=255)
                                                                                       cgcATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGt                                                                            <  4:34987/131‑1 (MQ=255)
                                                                                       cgcATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGt                                                                            >  3:34987/1‑131 (MQ=255)
                                                                                                   gAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTAt                                              >  6:215896/1‑149 (MQ=255)
                                                                                                       gTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTAt                                              >  4:106113/1‑145 (MQ=255)
                                                                                                       gTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTAt                                              <  3:106113/145‑1 (MQ=255)
                                                                                                            cTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCAccc                                     <  4:257732/149‑1 (MQ=255)
                                                                                                             ttCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCca                                    >  2:53838/1‑149 (MQ=255)
                                                                                                                 ggCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACAcc                                >  8:48379/1‑149 (MQ=255)
                                                                                                                 ggCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACAcc                                >  8:103395/1‑149 (MQ=255)
                                                                                                                  gCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCt                               >  4:177282/1‑149 (MQ=255)
                                                                                                                  gCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCt                               <  8:62800/149‑1 (MQ=255)
                                                                                                                  gCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCt                               >  2:92125/1‑149 (MQ=255)
                                                                                                                    cAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTc                                                           <  1:19754/119‑1 (MQ=255)
                                                                                                                    cAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTc                                                           >  2:19754/1‑119 (MQ=255)
                                                                                                                     aGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTggg                            <  1:53838/149‑1 (MQ=255)
                                                                                                                         ttGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCGCACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCACCGACACCGGTGGCCCCGGTCATGTTCTGCGTATCGATCACCCACACCTGGCTCtt                        >  5:195461/1‑149 (MQ=255)
                                                                                                                            aGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGc                      <  1:92125/148‑1 (MQ=255)
                                                                                                                                 gTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCAc                                                               >  2:1010/1‑102 (MQ=255)
                                                                                                                                 gTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCAc                >  4:230587/1‑149 (MQ=255)
                                                                                                                                      cGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTc            >  5:29968/1‑148 (MQ=255)
                                                                                                                                       gCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCa                                        >  4:77019/1‑119 (MQ=255)
                                                                                                                                       gCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCa                                        <  3:77019/119‑1 (MQ=255)
                                                                                                                                        ccGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAg         >  6:207431/1‑149 (MQ=255)
                                                                                                                                          cccggTGAGCGCGTGGTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGc        >  7:36850/3‑148 (MQ=255)
                                                                                                                                            cggTGAGCGCGTGGTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTAt                                              >  5:37176/1‑108 (MQ=255)
                                                                                                                                            cggTGAGCGCGTGGTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTAt                                              <  6:37176/108‑1 (MQ=255)
                                                                                                                                            cggTGAGCGCGTGGTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTc            >  4:102267/1‑142 (MQ=255)
                                                                                                                                            cggTGAGCGCGTGGTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTc            <  3:102267/142‑1 (MQ=255)
                                                                                                                                            cggTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCATCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAg     <  5:215896/149‑1 (MQ=255)
                                                                                                                                            cggTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAg     <  7:103395/149‑1 (MQ=255)
                                                                                                                                               tGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGa          >  8:140407/1‑141 (MQ=255)
                                                                                                                                               tGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGa          <  7:140407/141‑1 (MQ=255)
                                                                                                                                               tGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAGGCa  <  5:207431/149‑1 (MQ=255)
                                                                                                                                               tGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAGGCa  >  8:239773/1‑149 (MQ=255)
                                                                                                                                               tGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAGGCa  >  5:240186/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CCATCAACAGGCTAACGAACATCAGGTACCAGACGAACGATTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAGGCA  >  NC_002947/5900803‑5901094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑