Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,900,954 C→T 8.9% R333H (CGC→CAC)  PP_5174 ← RND family transporter membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,900,9540CT 8.9% 155.0 / 4.7 56R333H (CGC→CAC) PP_5174RND family transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base C (26/25);  new base T (3/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-01

CTAACGAACATCAGGTACCAGACGAACGATTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAGGCACTCAAGGG  >  NC_002947/5900814‑5901102
                                                                                                                                            |                                                                                                                                                    
cTAACGAACATCAGGTACCAGACGAACGATTGTTGCTGGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGAACACGCGCTCACCg                                                                                                                                              <  4:153249/149‑1 (MQ=255)
 tAACGAACATCAGGTACCAGACGAACGATTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCgg                                                                                                                                             >  3:257732/1‑149 (MQ=255)
                      cgaacgaTTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCa                                                                                                                        <  3:159102/149‑1 (MQ=255)
                       gaacgaTTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGc                                                                                                                                           <  3:46476/129‑1 (MQ=255)
                        aacgaTTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCAc                                                                                                                       >  3:79679/1‑148 (MQ=255)
                             ttGATGCTTGAGTGCCCAGTCGGACAGGTTTAAGCTTCCTTTCATTGCTCATCCTAGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGACGGCGGTGACCACGCGTGCACCGGGCTGCACACCGGCGATCAGCACGATGCt                                                                                                                 <  1:40627/149‑1 (MQ=255)
                                    ttGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCGTTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCgg                                                                                                                                 <  5:223762/126‑1 (MQ=255)
                                    ttGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCgg                                                                                                                                 >  6:223762/1‑126 (MQ=255)
                                      gAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAgcg                                                                                                          <  6:60238/147‑1 (MQ=255)
                                        gTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAgcggtgc                                                                                                      <  3:64927/149‑1 (MQ=255)
                                            ccAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTc                                                                                                  >  7:62800/1‑149 (MQ=255)
                                             cAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCACTCAGCACGATGCTGTCAGCGGTGCGGTCg                                                                                                 <  2:130519/149‑1 (MQ=255)
                                                  ggACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCCTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAg                                                                                            >  6:199803/1‑149 (MQ=255)
                                                        gTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCg                                                                                                                                  >  7:32698/1‑105 (MQ=255)
                                                        gTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCg                                                                                                                                  <  8:32698/105‑1 (MQ=255)
                                                         ttGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAgcggtgcggt                                                                                                   >  7:7510/1‑135 (MQ=255)
                                                         ttGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAgcggtgcggt                                                                                                   <  8:7510/135‑1 (MQ=255)
                                                             aGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCAc                                                                                 <  8:84690/149‑1 (MQ=255)
                                                                        aTTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAgcggt                                                                                                        >  1:222547/1‑115 (MQ=255)
                                                                        aTTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAgcggt                                                                                                        <  2:222547/115‑1 (MQ=255)
                                                                           gcgcATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGt                                                                    <  4:79679/148‑1 (MQ=255)
                                                                            cgcATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGt                                                                                    >  3:34987/1‑131 (MQ=255)
                                                                            cgcATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGt                                                                                    <  4:34987/131‑1 (MQ=255)
                                                                                        gAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTAt                                                      >  6:215896/1‑149 (MQ=255)
                                                                                            gTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTAt                                                      >  4:106113/1‑145 (MQ=255)
                                                                                            gTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTAt                                                      <  3:106113/145‑1 (MQ=255)
                                                                                                 cTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCAccc                                             <  4:257732/149‑1 (MQ=255)
                                                                                                  ttCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCca                                            >  2:53838/1‑149 (MQ=255)
                                                                                                      ggCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACAcc                                        >  8:48379/1‑149 (MQ=255)
                                                                                                      ggCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACAcc                                        >  8:103395/1‑149 (MQ=255)
                                                                                                       gCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCt                                       <  8:62800/149‑1 (MQ=255)
                                                                                                       gCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCt                                       >  4:177282/1‑149 (MQ=255)
                                                                                                       gCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCt                                       >  2:92125/1‑149 (MQ=255)
                                                                                                         cAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTc                                                                   <  1:19754/119‑1 (MQ=255)
                                                                                                         cAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTc                                                                   >  2:19754/1‑119 (MQ=255)
                                                                                                          aGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTggg                                    <  1:53838/149‑1 (MQ=255)
                                                                                                              ttGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCGCACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCACCGACACCGGTGGCCCCGGTCATGTTCTGCGTATCGATCACCCACACCTGGCTCtt                                >  5:195461/1‑149 (MQ=255)
                                                                                                                 aGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGc                              <  1:92125/148‑1 (MQ=255)
                                                                                                                      gTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCAc                                                                       >  2:1010/1‑102 (MQ=255)
                                                                                                                      gTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCAc                        >  4:230587/1‑149 (MQ=255)
                                                                                                                           cGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTc                    >  5:29968/1‑148 (MQ=255)
                                                                                                                            gCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCa                                                <  3:77019/119‑1 (MQ=255)
                                                                                                                            gCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCa                                                >  4:77019/1‑119 (MQ=255)
                                                                                                                             ccGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAg                 >  6:207431/1‑149 (MQ=255)
                                                                                                                               cccggTGAGCGCGTGGTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGc                >  7:36850/3‑148 (MQ=255)
                                                                                                                                 cggTGAGCGCGTGGTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTAt                                                      >  5:37176/1‑108 (MQ=255)
                                                                                                                                 cggTGAGCGCGTGGTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTAt                                                      <  6:37176/108‑1 (MQ=255)
                                                                                                                                 cggTGAGCGCGTGGTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTc                    >  4:102267/1‑142 (MQ=255)
                                                                                                                                 cggTGAGCGCGTGGTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTc                    <  3:102267/142‑1 (MQ=255)
                                                                                                                                 cggTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCATCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAg             <  5:215896/149‑1 (MQ=255)
                                                                                                                                 cggTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAg             <  7:103395/149‑1 (MQ=255)
                                                                                                                                    tGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGa                  <  7:140407/141‑1 (MQ=255)
                                                                                                                                    tGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGa                  >  8:140407/1‑141 (MQ=255)
                                                                                                                                    tGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAGGCa          <  5:207431/149‑1 (MQ=255)
                                                                                                                                    tGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAGGCa          >  8:239773/1‑149 (MQ=255)
                                                                                                                                    tGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAGGCa          >  5:240186/1‑149 (MQ=255)
                                                                                                                                            cgcTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAGGCACTCAAggg  <  6:240186/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
CTAACGAACATCAGGTACCAGACGAACGATTGATGCTTGAGTGCCCAGTCGGACAGGTTGAAGCTTCCTTTCATTGCGCATCCTCGTCGAAGGTGACCTTCTGGCCAGGCTTGAGGCTGTTGACGCCGGCGGTGACCACGCGCTCACCGGGCTGCACACCGGCGCTCAGCACGATGCTGTCAGCGGTGCGGTCGATCAGCGCCACGTCACGGGTGGCCACGGTCTTGTTCTGCGTATCGATCACCCACACCTGGGTCTTGCCATCACGCTCGAGCAAGGCACTCAAGGG  >  NC_002947/5900814‑5901102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑