Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,919,685 C→G 9.0% P91A (CCC→GCC) ‡ PP_5190 → type II secretion system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,919,6850CG 9.0% 165.7 / 4.7 53P91A (CCC→GCC) ‡PP_5190type II secretion system protein
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base G (2/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAGGCCGACCTGGCCCGCAGCCTCGGCCGGT  >  NC_002947/5919539‑5919832
                                                                                                                                                  |                                                                                                                                                   
gCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGc                                                                                                                                                     <  2:74604/147‑1 (MQ=255)
   aCCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAg                                                                                                                                                <  3:214687/149‑1 (MQ=255)
     ccGCTGGAGCACATCGCCGCCAGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGACCACCTTTGCCAGTGGCTCCCCGACAAGGTCGGCCAACCCTCCCTCCGCCTCGAACCCAAGCAACACGACCTGCCCCAGGt                                                                                                                                              >  3:11258/1‑149 (MQ=255)
            aGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGc                                                                                                                                       <  2:925/149‑1 (MQ=255)
             gCACATCGCGGGCTGCAGCCTGGAAAGCCGGCAGCACACCGGCCAGCCCCTGGACTTGGAGCACCTTTGGCAGTGGCTGGCCGACAAGGTCGGCCAACCCTTCCTGCGCATCGAGCACATTCAACTCGACCTGCTCCAGTTCACCAGc                                                                                                                                       <  8:213433/148‑1 (MQ=255)
                  tCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGAtc                                                                                                                                 <  7:155807/149‑1 (MQ=255)
                      cgccTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTccc                                                                                                                             >  5:158348/1‑149 (MQ=255)
                        ccTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCa                                                                                                                           <  6:158348/149‑1 (MQ=255)
                         cTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAg                                                                                                                          >  8:28523/1‑149 (MQ=255)
                           gCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGGGGCAGGTcgag                                                                                                                                          >  1:122631/1‑128 (MQ=255)
                           gCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGGGGCAGGTcgag                                                                                                                                          <  2:122631/131‑4 (MQ=255)
                              gCCTGGAACGCCGCCAGCACCCCGGCCTGCCCCTTGTGTTTGAGCACCTTTGCCAGTGGCTGGCGGACAAGGGCTGCCAACCCTACCTGCGCCTCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTc                                                                                                                     <  6:62299/149‑1 (MQ=255)
                                      aGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAgcg                                                                                                             <  4:226573/149‑1 (MQ=255)
                                       gccgccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGGGGCAGGTcgag                                                                                                                                          >  8:115281/1‑116 (MQ=255)
                                       gccgccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGGGGCAGGTcgag                                                                                                                                          <  7:115281/119‑4 (MQ=255)
                                         cgccAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCa                                                                                                          >  8:216287/1‑149 (MQ=255)
                                                aCCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATc                                                                                                   >  5:74475/1‑149 (MQ=255)
                                                aCCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATc                                                                                                   <  5:273182/149‑1 (MQ=255)
                                                 ccccGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCTTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATcc                                                                                                  >  1:91428/1‑149 (MQ=255)
                                                    cGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCa                                                                                               <  4:181031/149‑1 (MQ=255)
                                                              cTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCAAGCGCCACGGCATCCTCATCGTCGCGGc                                                                                     >  4:187785/1‑149 (MQ=255)
                                                                  aCTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGAt                                                                                 <  7:28523/149‑1 (MQ=255)
                                                                    ttGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATcc                                                                                                  >  3:182559/1‑130 (MQ=255)
                                                                    ttGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATcc                                                                                                  <  4:182559/130‑1 (MQ=255)
                                                                       gAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCTTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCt                                                                             <  2:91428/148‑1 (MQ=255)
                                                                        aGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTcc                                                                           <  7:216287/149‑1 (MQ=255)
                                                                         gCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTc                                                                            >  1:115524/1‑147 (MQ=255)
                                                                                    aGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTc                                                               <  2:115524/149‑1 (MQ=255)
                                                                                           ggcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgcg                                                        >  7:93428/1‑149 (MQ=255)
                                                                                            gcCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgcg                                                        <  6:74475/148‑1 (MQ=255)
                                                                                              cGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGcc                                                      >  7:124873/1‑148 (MQ=255)
                                                                                                        ggCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCa                                           <  3:163992/149‑1 (MQ=255)
                                                                                                          ccAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGcc                                                                                                                  <  6:44653/76‑1 (MQ=255)
                                                                                                          ccAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGcc                                                                                                                  >  5:44653/1‑76 (MQ=255)
                                                                                                            aaCCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgc                                                         <  4:21524/131‑1 (MQ=255)
                                                                                                            aaCCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgc                                                         <  4:234186/131‑1 (MQ=255)
                                                                                                            aaCCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgc                                                         >  3:21524/1‑131 (MQ=255)
                                                                                                            aaCCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAgc                                                         >  3:234186/1‑131 (MQ=255)
                                                                                                              cccTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATc                                                                                                   >  4:221654/1‑87 (MQ=255)
                                                                                                              cccTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATc                                                                                                   <  3:221654/87‑1 (MQ=255)
                                                                                                                cTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACt                                   <  8:93428/149‑1 (MQ=255)
                                                                                                                      gcgcATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAg                                          >  7:192147/1‑136 (MQ=255)
                                                                                                                      gcgcATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAg                                          <  8:192147/136‑1 (MQ=255)
                                                                                                                      gcgcATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAgg                             >  6:257700/1‑149 (MQ=255)
                                                                                                                              agcccATTCAACTCGAGCTGGCCACGGTCACGAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGGGGCCGATGCCTCGAGCGTCACCGTCGCCAGCGCCCAGTCTTTCCAGGATGACTGGCAGGCCGACGTg                     <  4:11258/147‑1 (MQ=255)
                                                                                                                                 ccATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAGGCCGACCTGGcc                  >  3:105960/1‑149 (MQ=255)
                                                                                                                                  cATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAGGCCGACCTGGccc                 >  4:198983/1‑149 (MQ=255)
                                                                                                                                          tCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAGGCCGACCTGGCCCg                <  2:238048/142‑1 (MQ=255)
                                                                                                                                          tCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAGGCCGACCTGGCCCg                >  1:238048/1‑142 (MQ=255)
                                                                                                                                          tCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAGGCCGACCTGGCCCGCAGCCTc         >  8:10745/1‑149 (MQ=255)
                                                                                                                                          tCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAGGCCGACCTGGCCCGCAGCCTc         >  1:58709/1‑149 (MQ=255)
                                                                                                                                            gACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAGGCCGACCTGGCCCGCAGCCTCgg       >  5:227494/1‑149 (MQ=255)
                                                                                                                                                 gCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAGGcc                           >  2:199912/1‑124 (MQ=255)
                                                                                                                                                 gCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAGGcc                           <  1:199912/124‑1 (MQ=255)
                                                                                                                                                  ccccAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAGGCCGACCTGGCCCGCAGCCTCGGCCGGt  <  2:58709/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTGGAAAGCCGCCAGCACCCCGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGACAAGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTTTCCAGGATGACTGGCAGGCCGACCTGGCCCGCAGCCTCGGCCGGT  >  NC_002947/5919539‑5919832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑