Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	482,571	T→C	7.7%	D623G (GAC→GGC)	yeaG ←	protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	482,571	0	T	C	7.7%	149.0 / 2.7	52	D623G (GAC→GGC)	yeaG	protein kinase
Reads supporting (aligned to +/- strand):  ref base T (21/27);  new base C (2/2);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

AAGAGCAGCCACCGCCGCTGCAAGCTGTGACGCGGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACATTTTCTTCTCGATCACGACCCGCAGCTTTT  >  NC_002947/482428‑482718                                                                                                                                                |                                                                                                                                                    aagaGCAGCCACCGCCGCTGCAAGCTGTGACGCGGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGt                                                                                                                                                 <  8:213070/148‑1 (MQ=255)      cagcCACCGCCGCTGCAAGCTGTGACGCGGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGcc                                                                                                                                           <  2:7137/149‑1 (MQ=255)              gccgcTGCAAGCTGTGACGCGGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTc                                                                                                                                     >  4:254058/1‑147 (MQ=255)              gccgcTGCAAGCTGTGACGCGGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTc                                                                                                                                     <  3:254058/147‑1 (MQ=255)                  cTGCAAGCTGTGACGCGGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCAt                                                                                                                               >  3:257245/1‑149 (MQ=255)                        gCTGTGACGCGGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGt                                                                                                                         <  6:28897/149‑1 (MQ=255)                        gCTGTGACGCGGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGt                                                                                                                         >  2:216456/1‑149 (MQ=255)                              aCGCGGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACg                                                                                                                     >  4:117024/1‑147 (MQ=255)                              aCGCGGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACg                                                                                                                     >  4:112939/1‑147 (MQ=255)                                gcgGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGt                                                                                                                 >  6:156189/1‑149 (MQ=255)                                gcgGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGt                                                                                                                 >  5:222567/1‑149 (MQ=255)                                    gcagcaATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGtt                                                                                                             <  7:223235/149‑1 (MQ=255)                                    gcagcaATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGtt                                                                                                             <  2:83693/149‑1 (MQ=255)                                      agcaATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTc                                                                                                                <  8:92640/144‑1 (MQ=255)                                      agcaATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTc                                                                                                                >  7:92640/1‑144 (MQ=255)                                         aaTGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTtgt                                                                                                           <  2:149786/146‑1 (MQ=255)                                         aaTGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTtgt                                                                                                           >  1:149786/1‑146 (MQ=255)                                             ccAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTttctt                                                                                                    >  2:124982/1‑149 (MQ=255)                                             ccAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTttctt                                                                                                    <  1:216456/149‑1 (MQ=255)                                                 cGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTgg                                                                                                >  7:125560/1‑149 (MQ=255)                                                 cGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTgg                                                                                                <  7:144306/149‑1 (MQ=255)                                                    cAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGt                                                                                                              >  3:101644/1‑132 (MQ=255)                                                    cAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGt                                                                                                              <  4:101644/132‑1 (MQ=255)                                                     aGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTtgtg                                                                                                          <  2:147761/135‑1 (MQ=255)                                                     aGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTtgtg                                                                                                          >  1:147761/1‑135 (MQ=255)                                                              gcagcttgcGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTg                                                                                    <  2:166260/148‑1 (MQ=255)                                                                   ttgcGGCTGGTTGCTTCCCGCTTTATTGCGATTTCCTGACCCGCTGGTACCATTCCTACCGCTGGCGCACGTGTTTGTGGGAATAGCCCCGCTCCACCATCCGGGTGACGAAGTGGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTg                                                                              <  8:125560/149‑1 (MQ=255)                                                                     gcGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTgg                                                                             <  5:156189/148‑1 (MQ=255)                                                                            gTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGaa                                                                      <  4:257245/148‑1 (MQ=255)                                                                                cTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCtga                                                                 >  1:229375/1‑149 (MQ=255)                                                                                            ttGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGgcagc                                                      <  6:32392/148‑1 (MQ=255)                                                                                            ttGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGgcag                                                       >  5:193480/1‑147 (MQ=255)                                                                                                     ccTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCg                                             <  3:171636/148‑1 (MQ=255)                                                                                                       tGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGgtg                                          >  4:131447/1‑149 (MQ=255)                                                                                                       tGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGgtg                                          >  6:148036/1‑149 (MQ=255)                                                                                                                     cATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTgg                                      <  7:226428/139‑1 (MQ=255)                                                                                                                     cATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTgg                                      >  8:226428/1‑139 (MQ=255)                                                                                                                     cATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACAtttt                            <  3:112939/149‑1 (MQ=255)                                                                                                                     cATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACAtttt                            <  3:117024/149‑1 (MQ=255)                                                                                                                      aTTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACATTttc                           <  6:222567/149‑1 (MQ=255)                                                                                                                         ccGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACATTttctt                         <  3:131447/148‑1 (MQ=255)                                                                                                                              agcagGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACATTTTCTTCTCGAt                   <  6:193480/149‑1 (MQ=255)                                                                                                                               gcagGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACATTTTCTTCTCGATc                  <  4:54628/149‑1 (MQ=255)                                                                                                                                cagGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACATTttc                           <  5:239655/139‑1 (MQ=255)                                                                                                                                cagGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACATTttc                           >  6:239655/1‑139 (MQ=255)                                                                                                                                       aCCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACATTTTCTTCTCGATCACGACCCg          >  3:124665/1‑149 (MQ=255)                                                                                                                                       aCCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACATTTTCTTCTCGATCACGACCCg          <  1:124982/149‑1 (MQ=255)                                                                                                                                        ccTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACATTTTCTTCTCGATCACGACCCg          >  4:246755/1‑148 (MQ=255)                                                                                                                                            tttGCCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCg                                                           >  4:254299/1‑95 (MQ=255)                                                                                                                                            tttGCCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCg                                                           >  2:146675/1‑95 (MQ=255)                                                                                                                                            tttGCCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCg                                                           <  3:254299/95‑1 (MQ=255)                                                                                                                                            tttGCCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCg                                                           <  1:146675/95‑1 (MQ=255)                                                                                                                                                tCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACATTTTCTTCTCGATCACGACCCGCAGCtttt  <  2:229375/148‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    AAGAGCAGCCACCGCCGCTGCAAGCTGTGACGCGGGCAGCAATGCCAACGCCAGGCAGCTTGCAGCTTGCGGCTCGTAGCTTGCCGCTTTATTGCGATTTCCTGACCCGCAGGTACCATTCCGACAGCAGGCGCACCTGTTTGTCGGTATAGCCACGCTCCACCATCCGGGTGACGAAGTCGTTGTGTTTCTTCTGGTCCTCTTTGCTGGCCTTGGCGTTGAAGCTGATGACCGGCAGCAGGTCTTCGGTGTTGGAGAACATTTTCTTCTCGATCACGACCCGCAGCTTTT  >  NC_002947/482428‑482718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑