Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,925,399 T→C 9.7% N249S (AAC→AGC)  gcvT‑II ← aminomethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,925,3990TC 9.7% 167.2 / 8.0 62N249S (AAC→AGC) gcvT‑IIaminomethyltransferase
Reads supporting (aligned to +/- strand):  ref base T (29/27);  new base C (2/4);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-01

ACCTGATGGGCGCGCAGTACACCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATC  >  NC_002947/5925255‑5925544
                                                                                                                                                |                                                                                                                                                 
aCCTGATGGGCGCGCAGTACACCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGa                                                                                                                                               <  4:80646/149‑1 (MQ=255)
 ccTGATGGGCGCGCAGTACACCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGTCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAg                                                                                                                                              <  5:38310/149‑1 (MQ=255)
 ccTGATGGGCGCGCAGTACACCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAg                                                                                                                                              >  7:144600/1‑149 (MQ=255)
  cTGATGGGCGCGCAGTACACCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAACCTGGGCTGGAgc                                                                                                                                             <  6:52172/149‑2 (MQ=255)
   tGATGGGCGCGCAGTACACCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAg                                                                                                                                              >  1:87464/1‑147 (MQ=255)
   tGATGGGCGCGCAGTACACCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAg                                                                                                                                              <  2:87464/147‑1 (MQ=255)
     aTGGGCGCGCAGTACACCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGt                                                                                                                                            >  7:56449/1‑147 (MQ=255)
     aTGGGCGCGCAGTACACCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGt                                                                                                                                            <  3:3022/147‑1 (MQ=255)
     aTGGGCGCGCAGTACACCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAACCTGGGCTGGAgca                                                                                                                                            <  2:138147/147‑3 (MQ=255)
          cgcgcAGTACACCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCggg                                                                                                                                     <  4:25300/149‑1 (MQ=255)
                   cacCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGtt                                                                                                                             <  1:42307/148‑1 (MQ=255)
                      cgcgCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTtcg                                                                                                                         >  5:52800/1‑149 (MQ=255)
                             ttCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGt                                                                                                                  >  3:256379/1‑149 (MQ=255)
                               ccAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTc                                                                                                                 <  8:56449/148‑1 (MQ=255)
                                cAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCTGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCt                                                                                                               >  3:219294/1‑149 (MQ=255)
                                cAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTcc                                                                                                                <  8:241295/148‑1 (MQ=255)
                                cAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCt                                                                                                               >  7:80669/1‑149 (MQ=255)
                                 aGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAACCTGGGCTGGAgcatcgc                                                                                                                                        >  8:176045/1‑117 (MQ=255)
                                 aGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAACCTGGGCTGGAgcatcgc                                                                                                                                        <  7:176045/123‑7 (MQ=255)
                                     ccAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCg                                                                                                          >  6:41887/1‑149 (MQ=255)
                                      cAGACCGACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGt                                                                                                         <  8:125337/149‑1 (MQ=255)
                                           ccaccaGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAgg                                                                                                    >  3:125722/1‑149 (MQ=255)
                                               caGCCTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCa                                                                                                >  7:225258/1‑149 (MQ=255)
                                                       ccTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTtcgt                                                                                                                        <  5:211771/117‑1 (MQ=255)
                                                       ccTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTtcgt                                                                                                                        >  6:211771/1‑117 (MQ=255)
                                                        cTGCACGCCGTGCGCGATTTCCCCCAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCtt                                                                                       <  6:189945/149‑1 (MQ=255)
                                                          gCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTgg                                                                                                                               <  5:228247/107‑1 (MQ=255)
                                                          gCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTgg                                                                                                                               >  6:228247/1‑107 (MQ=255)
                                                            aCGCCGTGCTCGATTTCTGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAg                                                                                   <  4:219294/149‑1 (MQ=255)
                                                              gCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGgc                                                                                 <  8:144600/149‑1 (MQ=255)
                                                                       gATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTAt                                                                        >  1:58693/1‑149 (MQ=255)
                                                                        aTTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATc                                                                       >  8:52228/1‑149 (MQ=255)
                                                                        aTTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATc                                                                       <  5:41887/149‑1 (MQ=255)
                                                                         tttCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATcg                                                                      >  6:109589/1‑149 (MQ=255)
                                                                         tttCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAACCTGGGCTGGAgcat                                                                                                                                           >  7:39247/1‑77 (MQ=255)
                                                                         tttCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAACCTGGGCTGGAgcat                                                                                                                                           <  8:39247/80‑4 (MQ=255)
                                                                             cGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCgg                                                                   <  7:37270/148‑1 (MQ=255)
                                                                                    aGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAg                                                           >  6:109263/1‑149 (MQ=255)
                                                                                        cGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGCTGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCTCGGGCGCGCAGGCCg                                                       >  5:142273/1‑149 (MQ=255)
                                                                                          acacGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCt                                                     >  3:230504/1‑149 (MQ=255)
                                                                                           cacGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCtt                                                    <  8:80669/149‑1 (MQ=255)
                                                                                            acGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTg                                                   <  5:109589/149‑1 (MQ=255)
                                                                                              ggCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGc                                                         >  7:7985/1‑141 (MQ=255)
                                                                                              ggCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGc                                                         <  8:7985/141‑1 (MQ=255)
                                                                                              ggCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTggg                                                 <  7:52228/149‑1 (MQ=255)
                                                                                                ccGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGc                                               <  8:225258/149‑1 (MQ=255)
                                                                                                  gATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAgg                                                          >  4:20370/1‑136 (MQ=255)
                                                                                                  gATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGa                                             <  4:256379/149‑1 (MQ=255)
                                                                                                    tGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAgg                                                                                  <  5:71967/110‑1 (MQ=255)
                                                                                                    tGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAgg                                                                                  >  6:71967/1‑110 (MQ=255)
                                                                                                            cgcTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGgcgc                                    <  6:52800/148‑1 (MQ=255)
                                                                                                             gcTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGcc                                   >  4:238814/1‑148 (MQ=255)
                                                                                                                 ggCCGGTTCCCAGGGGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGAc                                >  3:84056/1‑147 (MQ=255)
                                                                                                                 ggCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGAc                                >  7:143975/1‑147 (MQ=255)
                                                                                                                 ggCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGAc                                >  2:242497/1‑147 (MQ=255)
                                                                                                                         cccAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGtt                       <  1:242497/148‑1 (MQ=255)
                                                                                                                           cAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTg                      >  8:120184/1‑147 (MQ=255)
                                                                                                                                gATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTgaa                    >  1:170746/1‑144 (MQ=255)
                                                                                                                                gATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTgaa                    <  2:170746/144‑1 (MQ=255)
                                                                                                                                     tCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCAcc          >  5:163428/1‑149 (MQ=255)
                                                                                                                                           ccAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGa    <  5:109263/149‑1 (MQ=255)
                                                                                                                                             aGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATc  >  5:243244/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ACCTGATGGGCGCGCAGTACACCGCGCTCTTCCAGCACCAGACCCACCAGCTTTTCCTGCACGCCGTGCTCGATTTCCGCAAGCAGGCCGACACGGCCGATGAAGTTGCGCTCGGCCGGTTCCCAGGCGATGCTCCAGCCCAGGTTGGAGGTGAGCGGGGTGTGGTTTTCGTCGATGTCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATC  >  NC_002947/5925255‑5925544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑