Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,929,247 C→T 7.0% E381K (GAG→AAG)  visC ← ubiquinone biosynthesis oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,929,2470CT 7.0% 167.2 / 2.0 57E381K (GAG→AAG) visCubiquinone biosynthesis oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (29/24);  new base T (2/2);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCACCTACCTAGGCGTTACCGGATGTTGCAAAGGACTGCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCGGCGCTCGTAACGGCTCAGCACCTT  >  NC_002947/5929106‑5929385
                                                                                                                                             |                                                                                                                                          
cTCACCTACCTAGGCGTTACCGGATGTTGCAAAGGACTGCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGc                                                                                                                                     >  3:28060/1‑149 (MQ=255)
cTCACCTACCTAGGCGTTACCGGATGTTGCAAAGGACTGCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGc                                                                                                                                     <  2:32674/149‑1 (MQ=255)
   acctacctAGGCGTTACCGGATGTTGCAAAGGACTGCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCtt                                                                                                                                   >  2:142143/1‑148 (MQ=255)
         ctaGGCGTTACCGGATGTTGCAAAGGACTGCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCg                                                                                                                            >  7:109575/1‑149 (MQ=255)
          taGGCGTTACCGGATGTTGCAAAGGACTGCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGc                                                                                                                           >  5:210124/1‑149 (MQ=255)
              cGTTACCGGATGTTGCAAAGGACTGCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGtt                                                                                                                       <  6:210124/149‑1 (MQ=255)
                            gCAAAGGACTGCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAgcgc                                                                                                          >  6:228031/1‑148 (MQ=255)
                              aaaGGACTGCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGATGTTGCTCAAACAGCGCAAc                                                                                                       >  8:197840/1‑149 (MQ=255)
                                  gACTGCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCa                                                                                                   <  4:154455/149‑1 (MQ=255)
                                    cTGCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGc                                                                                                 <  6:38451/149‑1 (MQ=255)
                                      gCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAAACAGCGCAACGGCAGCg                                                                                                >  7:52567/1‑148 (MQ=255)
                                       cTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCAACCAGCTTCAACCCGCTGTTGCGCACCCAGCGCAACGGCAGCGGa                                                                                              >  6:66037/1‑149 (MQ=255)
                                        tACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTgc                                                                                                                     >  3:197320/1‑125 (MQ=255)
                                        tACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTgc                                                                                                                     <  4:197320/125‑1 (MQ=255)
                                         aCGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATc                                                                                            >  2:199781/1‑149 (MQ=255)
                                          cGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCg                                                                                           <  7:20093/149‑1 (MQ=255)
                                           gCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTTCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCgg                                                                                          >  6:159058/1‑149 (MQ=255)
                                           gCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTTCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCgg                                                                                          >  6:162689/1‑149 (MQ=255)
                                               ccccGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTg                                                                                      >  1:6389/1‑149 (MQ=255)
                                                cccGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCCACCAGCGCAACGGCAGCGGATCGGCCTg                                                                                      >  7:130932/1‑148 (MQ=255)
                                                ccagCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAAACAGCGCAACGGCAGCGGATCGGCCTgg                                                                                     <  8:52567/146‑1 (MQ=255)
                                                    ccACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGaac                                                                                  >  3:99080/1‑148 (MQ=255)
                                                      aCGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAAGAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGaacaac                                                                               <  4:144935/149‑1 (MQ=255)
                                                      aCGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGaacaac                                                                               <  8:180201/149‑1 (MQ=255)
                                                              gCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGaa                                                                       <  1:142143/149‑1 (MQ=255)
                                                                    gCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTTCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAAGCTCTCGAAGCCCTc                                                                 <  5:162689/149‑1 (MQ=255)
                                                                    gCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTTCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTc                                                                 <  5:159058/149‑1 (MQ=255)
                                                                      cAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAAGAACCTCTCGAAGCCCTCCa                                                               >  4:214430/1‑149 (MQ=255)
                                                                         aTCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGa                                                                                    >  5:122558/1‑125 (MQ=255)
                                                                         aTCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGa                                                                                    <  6:122558/125‑1 (MQ=255)
                                                                            cGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATcgccg                                                         <  7:154344/149‑1 (MQ=255)
                                                                                    caccacTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAAc                                                 >  6:110479/1‑149 (MQ=255)
                                                                                      ccacTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGc                                               <  7:197840/149‑1 (MQ=255)
                                                                                         cTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTccacc                                                                                                                                        <  4:199013/57‑1 (MQ=255)
                                                                                         cTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTccacc                                                                                                                                        >  3:199013/1‑57 (MQ=255)
                                                                                           cAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGt                                          >  4:132909/1‑149 (MQ=255)
                                                                                            aaaCCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAAAGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGtt                                         <  5:228031/149‑1 (MQ=255)
                                                                                               cccAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAAc                                                                                                                >  5:195822/1‑75 (MQ=255)
                                                                                               cccAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAAc                                                                                                                <  6:195822/75‑1 (MQ=255)
                                                                                               cccAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTtgtg                                      >  4:97506/1‑149 (MQ=255)
                                                                                                ccAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGCCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTtgtgt                                     >  6:182648/1‑149 (MQ=255)
                                                                                                  aaCGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTgg                                   <  8:109575/149‑1 (MQ=255)
                                                                                                  aaCGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCAGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTgg                                   <  6:229890/149‑1 (MQ=255)
                                                                                                           cgcACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCg                          <  4:99080/149‑1 (MQ=255)
                                                                                                           cgcACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCg                          <  2:6389/149‑1 (MQ=255)
                                                                                                            gcACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCgg                         >  4:241539/1‑149 (MQ=255)
                                                                                                                  aCAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCGGCGCTCg                   >  7:70392/1‑149 (MQ=255)
                                                                                                                  aCAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCGGCGCTCg                   >  8:58442/1‑149 (MQ=255)
                                                                                                                        ccTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCTACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCGGCGCTCGTAACgg             >  2:21950/1‑149 (MQ=255)
                                                                                                                        ccTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCGGCGCTCGTAACgg             <  4:28060/149‑1 (MQ=255)
                                                                                                                              ccTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCGGCGCTCGTAACGGCTCAGc       <  5:182648/149‑1 (MQ=255)
                                                                                                                              ccTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCGGCGCTCGTAACGGCTCAGc       <  1:199781/149‑1 (MQ=255)
                                                                                                                               cTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCGGCGCTCGTAACGGCTCAGCa      >  8:100450/1‑149 (MQ=255)
                                                                                                                               cTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGAAACCAGCGCAACGGCAGCGGATAGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGATGTGTGGCATACGCCGGCGCTCGTAACGGCTCACCa      >  6:15406/1‑149 (MQ=255)
                                                                                                                                tCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCGGCGCTCGTAACGGCTCAGCAc     >  8:198705/1‑149 (MQ=255)
                                                                                                                                   ggCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCGGCGCTCGTAACGGCTCAGCACCtt  <  5:110479/149‑1 (MQ=255)
                                                                                                                                   ggCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCGGCGCTCGTAACGGCTCAGCACCt   >  7:181675/1‑148 (MQ=255)
                                                                                                                                            tCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAg                                            <  1:33091/98‑1 (MQ=255)
                                                                                                                                            tCCACCAGCTTCAACCCGCTGTTGCGCAACCAGAGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAg                                            >  2:33091/1‑98 (MQ=255)
                                                                                                                                             |                                                                                                                                          
CTCACCTACCTAGGCGTTACCGGATGTTGCAAAGGACTGCTACGCAGCCCCGCCACGCTCAAGCCTTGGCCAGATCCGGCAGGTCACCACTCAAACCCAACGCCTGGCGCACAAACAGCGCCTTGGCCTCCGGCATCTGCTCCACCAGCTTCAACCCGCTGTTGCGCAACCAGCGCAACGGCAGCGGATCGGCCTGGAACAACCTCTCGAAGCCCTCCATCGCCGCCATCAACGCCAGGTTGTGTGGCATACGCCGGCGCTCGTAACGGCTCAGCACCTT  >  NC_002947/5929106‑5929385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑