Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,939,634 G→A 11.6% G732D (GGC→GAC)  rbbA → ribosome‑associated ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,939,6340GA11.6% 109.8 / 5.4 43G732D (GGC→GAC) rbbAribosome‑associated ATPase
Reads supporting (aligned to +/- strand):  ref base G (19/19);  new base A (3/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

ACCGCAGCCACGCCAGGACCCGGTGCGCCTGGAAACGCGTTTCCGCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCTACCTGGCTGTGAGCCTGGTCAACCTGGCGCTGCTGGTGGCGATGAACCGCTGG  >  NC_002947/5939497‑5939779
                                                                                                                                          |                                                                                                                                                  
aCCGCAGCCACGCCAGGACCCGGTGCGCCTGGAAACGCGTTTCCGCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTgcgc                                                                                                                                          >  4:80732/1‑148 (MQ=255)
       ccACGCCAGGACCCGGTGCGCCTGGAAACGCGTTTCCGCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAg                                                                                                                                   >  7:160061/1‑147 (MQ=255)
                  cgcGGTGCGCCTGGAAAGGCGTTTCCGCTACAACGAGGATGTGGTCAGCGTGAAGGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCTTTCCGGCCATGCTCACGGCACTggg                                                                                                                                                    <  8:221259/118‑1 (MQ=255)
                  cccGGTGCGCCTGGAAACGCGTTTCCGCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGACTTCATTCCGGCCATGCTCACGGCCCTggg                                                                                                                                                    >  7:221259/1‑120 (MQ=255)
                   ccGGTGCGCCTGGAAACGCGTTTCCGCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCAt                                                                                                                     >  6:42784/1‑149 (MQ=255)
                          gcCTGGAAACGCGTTTCCGCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAAc                                                                                                              <  7:75019/149‑1 (MQ=255)
                            cTGGAAACGCGTTTCCGCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACtt                                                                                                            >  1:251158/1‑149 (MQ=255)
                            cTGGAAACGCGTTTCCGCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGACATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACtt                                                                                                            >  4:41826/1‑149 (MQ=255)
                            cTGGAAACGCGTTTCCGCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGACATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACt                                                                                                             >  8:98956/1‑148 (MQ=255)
                                  aCGCGTTTCCGCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCGTCACCAACTTCTACGc                                                                                                      <  4:244667/149‑1 (MQ=255)
                                          ccGCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACAcccc                                                                                              >  4:101722/1‑149 (MQ=255)
                                            gCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCt                                                                                             >  7:144363/1‑148 (MQ=255)
                                               aCAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGc                                                                                                                          <  7:56674/116‑1 (MQ=255)
                                               aCAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGc                                                                                                                          >  8:56674/1‑116 (MQ=255)
                                                 aaCCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGc                                                                                                      >  5:88259/1‑134 (MQ=255)
                                                 aaCCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGc                                                                                                      <  6:88259/134‑1 (MQ=255)
                                                   ccAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGcc                                                                                     >  3:7560/1‑149 (MQ=255)
                                                     aGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTg                                                                                   >  5:169622/1‑149 (MQ=255)
                                                             gTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTcc                                                                            <  8:144363/148‑1 (MQ=255)
                                                                aGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGACATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCt                                                                        <  3:41826/149‑1 (MQ=255)
                                                                aGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGACATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCt                                                                        <  7:98956/149‑1 (MQ=255)
                                                                 gCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCtt                                                                       <  2:251158/149‑1 (MQ=255)
                                                                   gTGAACGCCATCGGCCCGGGCGTGATGGCACTGATGACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTg                                                                      >  1:40370/1‑149 (MQ=255)
                                                                   gTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTgg                                                                     <  2:64610/149‑1 (MQ=255)
                                                                        cGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGc                                                                <  6:169622/149‑1 (MQ=255)
                                                                         gCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAg                                                                 >  2:61211/1‑147 (MQ=255)
                                                                          ccATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAg                                                              <  1:61211/149‑1 (MQ=255)
                                                                          ccATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAg                                                              <  3:101722/149‑1 (MQ=255)
                                                                           cATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCATCCGCCTGGAATTCCTGCTTGGCAAg                                                                 >  8:82701/1‑145 (MQ=255)
                                                                            aTCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGAt                                                            <  8:188957/149‑1 (MQ=255)
                                                                                 cccGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGACATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGccc                                                        >  3:129576/1‑148 (MQ=255)
                                                                                        gTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGaa                                                                                >  5:164473/1‑117 (MQ=255)
                                                                                        gTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGaa                                                                                <  6:164473/117‑1 (MQ=255)
                                                                                            tGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCTACCTGGCtgt                                             <  5:42784/148‑1 (MQ=255)
                                                                                              gCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCTACCTGGCTGTGa                                           <  2:262894/148‑1 (MQ=255)
                                                                                                aCTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCt                                                       >  6:2344/1‑134 (MQ=255)
                                                                                                aCTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCt                                                       <  5:2344/134‑1 (MQ=255)
                                                                                                    aT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCTACCTGGCTGTGAGCCTGGt                                    <  2:40370/149‑1 (MQ=255)
                                                                                                         tGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCTACCTGGCTGTGAGCCTGGTCAAc                                >  4:63179/1‑149 (MQ=255)
                                                                                                                          aTGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCTACCTGGCTGTGAGCCTGGTCAACCTGGCGCTGCTGGTGGc               <  3:63179/149‑1 (MQ=255)
                                                                                                                           tGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCTACCTGGCTGTGAGCCTGGTCAACCTGGCGCTGCTGGTGGc               >  2:121617/1‑148 (MQ=255)
                                                                                                                             cTCACGGCACTGGGCATCGTGCGCGGAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCCCCCCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCTACCTGGCTGTGAGCCTGGTCAACCTGGAGCTGCTGGTGGCGa             >  7:167210/1‑149 (MQ=255)
                                                                                                                             cTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCTACCTGGCTGTGAGCCTGGTCAACCTGGCGCTGCTGGTGGCGAt            <  6:3165/149‑1 (MQ=255)
                                                                                                                              tCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCTACCTGGCTGTGAGCCTg                                      <  4:15538/122‑1 (MQ=255)
                                                                                                                              tCACGGCACTGGGCATCCTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACACCTCACCCGCCTGGAATTCCTGCTTCGCAAGCAGATGACCTACCTGGCTCTGATCCTGGTCAACCTGGCGCTGCTGGTGGCCAt            >  7:55326/1‑148 (MQ=255)
                                                                                                                                         ggCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCTACCTGGCTGTGAGCCTGGTCAACCTGGCGCTGCTGGTGGCGATGAACCgctgg  <  4:7560/147‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                  
ACCGCAGCCACGCCAGGACCCGGTGCGCCTGGAAACGCGTTTCCGCTACAACCAGGATGTGGTCAGCGTGAACGCCATCGGCCCGGGCGTGATGGCACTGAT‑ACTGGCCTTCATTCCGGCCATGCTCACGGCACTGGGCATCGTGCGC‑GAGAAGGAGCTCGGCTCCATCACCAACTTCTACGCCACACCCCTCACCCGCCTGGAATTCCTGCTTGGCAAGCAGATGCCCTACCTGGCTGTGAGCCTGGTCAACCTGGCGCTGCTGGTGGCGATGAACCGCTGG  >  NC_002947/5939497‑5939779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑