Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,941,091	G→A	8.7%	P310P (CCG→CCA)	yhhJ →	translation‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,941,091	0	G	A	8.7%	126.5 / 2.5	46	P310P (CCG→CCA)	yhhJ	translation‑like protein
Reads supporting (aligned to +/- strand):  ref base G (20/22);  new base A (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.61e-01

GCGCTGTACCTGTTCGCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGG  >  NC_002947/5940945‑5941239                                                                                                                                                   |                                                                                                                                                     gcgcTGTACCTGTTCGCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCa                                                                                                                                                    <  1:233795/149‑1 (MQ=255)     tGTACCTGTTCGCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTgg                                                                                                                                                <  1:271218/149‑1 (MQ=255)       tACCTGTTCGCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCt                                                                                                                                              >  1:93750/1‑149 (MQ=255)         ccTGTTCGCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTg                                                                                                                                             >  2:85316/1‑148 (MQ=255)           tGTTCGCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAg                                                                                                                                          >  4:192847/1‑149 (MQ=255)             ttCGCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTg                                                                                                                                        >  5:207626/1‑149 (MQ=255)             ttCGCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTg                                                                                                                                        >  3:235540/1‑149 (MQ=255)               cGCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTgg                                                                                                                                       <  6:207626/148‑1 (MQ=255)               cGCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTgg                                                                                                                                       <  3:137385/148‑1 (MQ=255)                gCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAg                                                                                                                                                   <  8:237959/135‑1 (MQ=255)                gCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAg                                                                                                                                                   >  7:237959/1‑135 (MQ=255)                         tCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATGATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAgg                                                                                                                            <  1:85316/149‑1 (MQ=255)                           gCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGt                                                                                                                          >  5:71431/1‑149 (MQ=255)                                    tttCCTCGCCACCATTGCGCGCTCGACGGCGCAGTTCGGCCTGCTGGCGATCCCGGTTCTCATCCCGATGCTGTCGCGGTCCGGCGGCAGCACACCTCTGGACACTAGGCCGCAGTGGCCGAAGTGGGTGATGCAAGGGGCACCCTCaa                                                                                                                 <  5:206143/149‑1 (MQ=255)                                      tCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACg                                                                                                               >  1:270662/1‑149 (MQ=255)                                        cTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCa                                                                                                             >  5:275673/1‑149 (MQ=255)                                         tCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCAc                                                                                                            >  2:267318/1‑149 (MQ=255)                                         tCGCCACCCTGGCGCGCTCGACGCCGCAGTACGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCACAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCAc                                                                                                            >  1:23171/1‑149 (MQ=255)                                           gCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACtt                                                                                                          >  4:95736/1‑149 (MQ=255)                                            ccACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACttt                                                                                                         <  2:93750/149‑1 (MQ=255)                                                 cTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAg                                                                                                    >  5:193086/1‑149 (MQ=255)                                                             aCGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGc                                                                                        <  2:108863/149‑1 (MQ=255)                                                             aCGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCACAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCg                                                                                         >  5:51212/1‑148 (MQ=255)                                                                   cAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCACAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCt                                                                                  <  2:23171/149‑1 (MQ=255)                                                                      ttCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGtt                                                                               <  6:71431/149‑1 (MQ=255)                                                                         ggCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCa                                                                             <  6:275673/148‑1 (MQ=255)                                                                         ggCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAg                                                                            >  7:229872/1‑149 (MQ=255)                                                                                    gATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGt                                                                 <  3:95736/149‑1 (MQ=255)                                                                                     aTCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCACAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTg                                                                <  6:51212/149‑1 (MQ=255)                                                                                           gTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCggt                                                          <  1:267318/149‑1 (MQ=255)                                                                                                  tcCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATgg                                                   <  6:193086/149‑1 (MQ=255)                                                                                                    ccGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGc                                                  <  4:235540/148‑1 (MQ=255)                                                                                                       aTGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGa                                              <  2:270662/149‑1 (MQ=255)                                                                                                                      ggcggcAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGAc                               >  3:118527/1‑149 (MQ=255)                                                                                                                          gcagcaCACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTg                           <  8:229872/149‑1 (MQ=255)                                                                                                                           cagcaCACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTg                           >  4:916/1‑148 (MQ=255)                                                                                                                            agcaCACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGAt                         >  2:123613/1‑149 (MQ=255)                                                                                                                            agcaCACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGAt                         >  2:119479/1‑149 (MQ=255)                                                                                                                                cacCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGGCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGc                     <  3:192847/149‑1 (MQ=255)                                                                                                                                 acCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCgg                                                           >  6:145875/1‑110 (MQ=255)                                                                                                                                 acCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGcc                    >  7:117427/1‑149 (MQ=255)                                                                                                                                   ctctGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCgg                                                           >  5:43607/1‑108 (MQ=255)                                                                                                                                   ctctGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCgg                                                           <  6:43607/108‑1 (MQ=255)                                                                                                                                   ctctGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTg                  <  1:119479/149‑1 (MQ=255)                                                                                                                                   ctctGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTg                  <  1:123613/149‑1 (MQ=255)                                                                                                                                           aGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGttctt          <  3:916/149‑1 (MQ=255)                                                                                                                                                   gCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTgg  >  6:32390/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GCGCTGTACCTGTTCGCCAGTACATCGCTGGGCATTTTCCTCGCCACCCTGGCGCGCTCGACGCCGCAGTTCGGCCTGCTGGCGATCCCGGTGATCATCCCGATGCTGTTGCTGTCCGGCGGCAGCACACCTCTGGACAGTATGCCGCAGTGGCTGCAGTGGGTGATGCAAGGGTCACCCTCAACGCACTTTGTCAGCCTGAGTGCCGCAATCCTGTTCAGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGG  >  NC_002947/5940945‑5941239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑