Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,963,846	T→G	10.3%	Y216D (TAT→GAT)	PP_5229 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,963,846	0	T	G	10.3%	220.8 / 16.5	78	Y216D (TAT→GAT)	PP_5229	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (37/33);  new base G (4/4);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

GCGTTCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTCG  >  NC_002947/5963699‑5963993                                                                                                                                                    |                                                                                                                                                     gCGTTCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCta                                                                                                                                                     <  2:262367/149‑1 (MQ=255)  cGTTCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCtat                                                                                                                                                    <  5:23370/149‑1 (MQ=255)  cGTTCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAAGCGCTGCATGGGGTTCTGGCCATCGGCAGTCGCGCCCCGCAACACTACAAGAGCAGCGTCGGAACCCTTTTCCTCGGCta                                                                                                                                                     >  4:38437/1‑148 (MQ=255)     tCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTAGCCTTTTCCTCGGCTATATc                                                                                                                                                 >  4:57132/1‑149 (MQ=255)        tGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGcc                                                                                                                                              >  6:242208/1‑149 (MQ=255)          ttCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCGATATAGcc                                                                                                                                              <  7:225731/147‑1 (MQ=255)            cGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCGATATAGCCGAg                                                                                                                                           <  6:96709/148‑1 (MQ=255)                           ccGGGAAGTAGGCTCCAGCGCCGGCGCCGCTCTTGAGTACCACCCGCTGCATGGGGTTCTGGCCATCCGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCTTtg                                                                                                                           <  6:162844/149‑1 (MQ=255)                            cGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTtgt                                                                                                                          <  6:146910/149‑1 (MQ=255)                                        tCCAGCGCCGTGGCAGCTCTTGGGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCAc                                                                                                              >  8:38289/1‑149 (MQ=255)                                          cAGCGCCGTGGCAGCTCTTGAGTATCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTg                                                                                                                                    >  5:200313/1‑125 (MQ=255)                                          cAGCGCCGTGGCAGCTCTTGAGTATCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTg                                                                                                                                    <  6:200313/125‑1 (MQ=255)                                          cAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCAcc                                                                                                             >  8:15222/1‑148 (MQ=255)                                          cAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTGCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCAcc                                                                                                             <  7:144325/148‑1 (MQ=255)                                             cgcCTTGGAAGCTCGTGAGTACCACGTGCTGCATGGGGTTCTGGCGATCGTCAGGATCGACCCGCAACACTACAAGAGCAGCGTCGGTACGCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCTTTGTGCCACGCGTCACCCA‑Ga                                                                                                        <  6:263049/149‑1 (MQ=255)                                               ccGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GAcc                                                                                                      <  8:155965/149‑1 (MQ=255)                                                 gttgCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGTTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCt                                                                                                    <  7:47538/146‑1 (MQ=255)                                                 gTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCt                                                                                                    <  5:177316/149‑1 (MQ=255)                                                    gCAGCGCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCCTCGGCAGCCGCGACCCGCCACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATCTCGCCGAGGTGCTTGGCCGCGGTGTGCCACGCGTCACCCA‑GACCCTgcg                                                                                                 <  5:62446/149‑1 (MQ=255)                                                        ctctTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCg                                                                                             <  5:133527/149‑1 (MQ=255)                                                         tctTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCGATATAGCCGAgga                                                                                                                                         >  3:45297/1‑104 (MQ=255)                                                         tctTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCGATATAGCCGAgga                                                                                                                                         <  4:45297/105‑2 (MQ=255)                                                              aGTACGAGGGGCTGCATGGGGTTCTGGCCATCGGCAGTCGCGACCCGCAACACTACAAGACCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGc                                                                                       <  3:38437/149‑1 (MQ=255)                                                               gTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCt                                                                                      >  3:245422/1‑149 (MQ=255)                                                               gTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCt                                                                                      >  1:192436/1‑149 (MQ=255)                                                                tACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCt                                                                                      >  2:128317/1‑148 (MQ=255)                                                                  ccAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑Ga                                                                                                        <  5:226240/128‑1 (MQ=255)                                                                  ccAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑Ga                                                                                                        >  6:226240/1‑128 (MQ=255)                                                                  ccAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGAGTCACCCA‑GACCCTGCGCCCGGTACGCtg                                                                                     >  5:1089/1‑147 (MQ=255)                                                                   cAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCtgat                                                                                   >  1:236123/1‑148 (MQ=255)                                                                     ggggCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCtgatga                                                                                 >  7:71435/1‑148 (MQ=255)                                                                      gggCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGaaa                                                                               <  8:163639/149‑1 (MQ=255)                                                                           gCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCa                                                                          <  5:71443/149‑1 (MQ=255)                                                                            cATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCa                                                                          <  4:245422/148‑1 (MQ=255)                                                                            cATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAg                                                                         >  4:119/1‑149 (MQ=255)                                                                              tGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCt                                                                       >  1:231204/1‑149 (MQ=255)                                                                                   ttCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAgg                                                                  <  1:128317/149‑1 (MQ=255)                                                                                   ttCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAgg                                                                  <  6:1089/149‑1 (MQ=255)                                                                                      tGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCCCGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCt                                                                >  6:200348/1‑148 (MQ=255)                                                                                        gCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTc                                                                                                                >  1:216480/1‑99 (MQ=255)                                                                                        gCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTc                                                                                                                <  2:216480/99‑1 (MQ=255)                                                                                        gCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTa                                                              <  3:119/148‑1 (MQ=255)                                                                                         ccATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTAtt                                                            <  5:242208/149‑1 (MQ=255)                                                                                          cATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCtga                                                                                    >  4:101179/1‑124 (MQ=255)                                                                                          cATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCtga                                                                                    <  3:101179/124‑1 (MQ=255)                                                                                          cATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTg                                                           >  7:63464/1‑149 (MQ=255)                                                                                            tCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTgcg                                                         <  7:15222/149‑1 (MQ=255)                                                                                               gCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGcac                                                      >  2:266069/1‑149 (MQ=255)                                                                                                   ccGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCtgatg                                                                                  >  1:33505/1‑117 (MQ=255)                                                                                                   ccGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCtgatg                                                                                  <  2:33505/117‑1 (MQ=255)                                                                                                            gCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAAc                                           >  4:31701/1‑147 (MQ=255)                                                                                                            gCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAAc                                           >  6:98718/1‑147 (MQ=255)                                                                                                                  cTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAgcgc                                     >  4:232471/1‑147 (MQ=255)                                                                                                                   tACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAgc                                       >  6:172819/1‑144 (MQ=255)                                                                                                                   tACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAgc                                       <  5:172819/144‑1 (MQ=255)                                                                                                                   tACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAg                                  >  2:176518/1‑149 (MQ=255)                                                                                                                   tACAAGAGAAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCTGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAg                                  >  5:217352/1‑149 (MQ=255)                                                                                                                     cAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAgg                                 <  5:98718/148‑1 (MQ=255)                                                                                                                     cAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAg                                  <  8:63464/147‑1 (MQ=255)                                                                                                                     cAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑AACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGgt                                >  6:159989/1‑149 (MQ=255)                                                                                                                       agagCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGgtgt                              >  5:196223/1‑149 (MQ=255)                                                                                                                            agcGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAg                         <  2:192436/149‑1 (MQ=255)                                                                                                                                tCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGcaca                     <  1:176518/149‑1 (MQ=255)                                                                                                                                   gTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCt                                                                                      >  2:97372/1‑81 (MQ=255)                                                                                                                                   gTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCt                                                                                      <  1:97372/81‑1 (MQ=255)                                                                                                                                   gTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCt                  >  5:274573/1‑149 (MQ=255)                                                                                                                                       ccTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCg                >  7:1719/1‑147 (MQ=255)                                                                                                                                       ccTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCg                >  8:171301/1‑147 (MQ=255)                                                                                                                                        cTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAg             >  7:233427/1‑149 (MQ=255)                                                                                                                                          tttCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAggg           >  1:238784/1‑149 (MQ=255)                                                                                                                                           ttCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAggg           <  8:233427/148‑1 (MQ=255)                                                                                                                                              cTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGcac                      >  7:145252/1‑134 (MQ=255)                                                                                                                                              cTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGcac                      <  8:145252/134‑1 (MQ=255)                                                                                                                                              cTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCATGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTa        >  1:202240/1‑149 (MQ=255)                                                                                                                                              cTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTAc       >  6:37798/1‑149 (MQ=255)                                                                                                                                               tCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTAcc      >  3:245623/1‑149 (MQ=255)                                                                                                                                               tCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTgc                                                <  5:20554/107‑1 (MQ=255)                                                                                                                                               tCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTgc                                                >  6:20554/1‑107 (MQ=255)                                                                                                                                                 ggCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGt    >  6:207741/1‑149 (MQ=255)                                                                                                                                                  gCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTc   <  2:236123/149‑1 (MQ=255)                                                                                                                                                   cTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTcg  <  3:31701/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCGTTCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCA‑GACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTCG  >  NC_002947/5963699‑5963993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑