Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,980,387 C→G 6.4% A344A (GCC→GCG)  PP_5242 → GAF domain‑containing sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,980,3870CG 6.4% 197.6 / 3.5 62A344A (GCC→GCG) PP_5242GAF domain‑containing sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (28/30);  new base G (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

CGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCCAAGAATAGTCAGGACCTCGTCACC  >  NC_002947/5980246‑5980531
                                                                                                                                             |                                                                                                                                                
cGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCa                                                                                                                                           <  1:8486/149‑1 (MQ=255)
   ccACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAgg                                                                                                                                        <  7:106843/149‑1 (MQ=255)
           gcCTGTTTGGCCGCGGAGCCGAGCAAAAGGCTATCACCCTGCTGCTAAAGGCCTTGAATCGCGATAGCACACCACAAGCCCTGCTCAGCAACGGCGCTGCTGGAATGGGCTAGTCGAGCCTGGGCGACGCCGCTCTCAAGGCCAACGcc                                                                                                                                >  8:241061/1‑149 (MQ=255)
                tttGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAgg                                                                                                                           <  8:105536/149‑1 (MQ=255)
                 ttGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAATCCGCTCTCAAGGCCAACGCCGAAGGt                                                                                                                          >  8:223552/1‑149 (MQ=255)
                    gCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTAc                                                                                                                       <  2:21985/149‑1 (MQ=255)
                     ccGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACt                                                                                                                      >  4:115257/1‑149 (MQ=255)
                             ccGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTc                                                                                                              >  8:151665/1‑149 (MQ=255)
                              cGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAACCCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCg                                                                                                             >  7:46524/1‑149 (MQ=255)
                               gAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCgg                                                                                                            >  3:9737/1‑149 (MQ=255)
                                     aaGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGaa                                                                                                                             <  5:214724/126‑1 (MQ=255)
                                     aaGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGaa                                                                                                                             >  6:214724/1‑126 (MQ=255)
                                        gCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCaa                                                                                                   <  3:115257/149‑1 (MQ=255)
                                        gCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCaa                                                                                                   <  7:223552/149‑1 (MQ=255)
                                            tCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACa                                                                                                 <  2:198229/147‑1 (MQ=255)
                                            tCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGc                                                                                               <  1:12742/149‑1 (MQ=255)
                                               cccTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCcac                                                                                            <  3:143331/149‑1 (MQ=255)
                                               cccTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCca                                                                                             >  8:16929/1‑148 (MQ=255)
                                                       ctCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGTTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCacac                                                                                          <  7:78544/143‑1 (MQ=255)
                                                       ctCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGTTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCacac                                                                                          >  8:78544/1‑143 (MQ=255)
                                                        tCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAg                                                                                     >  3:202723/1‑147 (MQ=255)
                                                        tCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAg                                                                                     >  3:129143/1‑147 (MQ=255)
                                                        tCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAg                                                                                     >  6:109334/1‑147 (MQ=255)
                                                          aaGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAACCAGt                                                                                 >  5:260744/1‑149 (MQ=255)
                                                             gCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAg                                                                                                                                         >  6:115622/1‑90 (MQ=255)
                                                             gCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAg                                                                                                                                         <  5:115622/90‑1 (MQ=255)
                                                               cTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTCGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGccc                                                                             >  3:232997/1‑148 (MQ=255)
                                                                ttGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGc                                                                                                                                                <  3:170279/80‑1 (MQ=255)
                                                                ttGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGc                                                                                                                                                >  4:170279/1‑80 (MQ=255)
                                                                ttGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTa                                                                           >  3:67012/1‑149 (MQ=255)
                                                                              cAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATc                                                              >  3:244512/1‑148 (MQ=255)
                                                                              cAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATc                                                              >  6:38166/1‑148 (MQ=255)
                                                                                   cacaAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGa                                                                <  5:113278/141‑1 (MQ=255)
                                                                                   cacaAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGa                                                                >  6:113278/1‑141 (MQ=255)
                                                                                   cacaAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATa                                                        >  8:132487/1‑149 (MQ=255)
                                                                                   cacaAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATa                                                        <  7:151665/149‑1 (MQ=255)
                                                                                              gTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGcc                                               <  5:109334/147‑1 (MQ=255)
                                                                                              gTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGcc                                               <  5:113464/147‑1 (MQ=255)
                                                                                              gTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCt                                             <  4:244512/149‑1 (MQ=255)
                                                                                                 atcaACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGa                                           <  4:129143/148‑1 (MQ=255)
                                                                                                    aaCGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCGGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTAc                                       >  6:109936/1‑149 (MQ=255)
                                                                                                    aaCGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCGGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTAc                                       >  6:105725/1‑149 (MQ=255)
                                                                                                         cgcTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGt                                  <  5:38166/149‑1 (MQ=255)
                                                                                                              ctgGCATGGGCAAGTCGAGCCTGGTCGAAGCGGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTg                             <  5:109936/149‑1 (MQ=255)
                                                                                                              ctgGCATGGGCAAGTCGAGCCTGGTCGAAGCGGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTg                             <  5:105725/149‑1 (MQ=255)
                                                                                                               tgGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTAAGTGCCCTGACTACCCAGTTGCTgg                            >  7:108503/1‑149 (MQ=255)
                                                                                                                 gCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCG‑AGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGcc                          >  6:208715/1‑148 (MQ=255)
                                                                                                                  cATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCG‑AGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCCaa                        <  5:208715/149‑1 (MQ=255)
                                                                                                                   aTGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCCaa                        <  4:67012/149‑1 (MQ=255)
                                                                                                                      ggCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGc                               <  4:6156/139‑1 (MQ=255)
                                                                                                                      ggCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGc                               >  3:6156/1‑139 (MQ=255)
                                                                                                                        cAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATCCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCCAAGAATa                   <  8:46524/149‑1 (MQ=255)
                                                                                                                         aaGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGaa                                                           <  3:20874/108‑1 (MQ=255)
                                                                                                                         aaGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGaa                                                           >  4:20874/1‑108 (MQ=255)
                                                                                                                         aaGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCCAAGAATAg                  >  3:178956/1‑149 (MQ=255)
                                                                                                                          aGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCCAAGAATAGt                 <  7:132487/149‑1 (MQ=255)
                                                                                                                          aGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCCAAGAATAGt                 <  6:260744/149‑1 (MQ=255)
                                                                                                                            tCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCCAAGAATAGt                 >  1:18490/1‑147 (MQ=255)
                                                                                                                              gtgCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGCGCCGTCGTCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCCAAGAATAGTCAg              <  8:237001/146‑1 (MQ=255)
                                                                                                                                gCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCCAAGAATAGTCAGGAc           <  4:202723/149‑1 (MQ=255)
                                                                                                                                      tCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCGAACAATAGTCAGGACCTCGt      >  3:74681/1‑148 (MQ=255)
                                                                                                                                         aaGCCGCTCTCAAGGCCAACGCCGAATGTTACTGGGCCTTCGGCAAATGGAACAGCCACACCCAAGCAGTGCCCTACACGCCCTTGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCCAAGAATAGTCAGGACCTCGTCAcc  <  7:241061/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                
CGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAACCACAAGCCCTGGTCATCAACGGCGCTGCTGGCATGGGCAAGTCGAGCCTGGTCGAAGCCGCTCTCAAGGCCAACGCCGAAGGTTACTGGGCCGTCGGCAAATGCAACAGCCACACCCAAGCAGTGCCCTACACGCCCTGGATCGAAATACTCAGTGCCCTGACTACCCAGTTGCTGGCCAAGAATAGTCAGGACCTCGTCACC  >  NC_002947/5980246‑5980531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑