| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,988,819 | T→C | 27.3% | intergenic (+113/‑433) | kefB‑III → / → PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,988,819 | 0 | T | C | 27.3% | 91.6 / 33.8 | 55 | intergenic (+113/‑433) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base T (16/24); new base C (7/8); total (23/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGA > NC_002947/5988680‑5988965 | ggaggtgttcgccgggtgattttcagcgccggggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCgggattt < 1:247450‑M2/62‑4 (MQ=255)cGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGAcgcg > 6:116761/1‑148 (MQ=255) tttGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGa < 8:142428/149‑1 (MQ=255) tGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAg < 5:116761/148‑1 (MQ=255) gATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCATACGT‑‑T‑TGTTTCGGGCAAGTTATTCCTGTGGGACGAGCACGCGAGcc > 8:103797/1‑149 (MQ=255) tttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGcat > 6:95135‑M2/66‑148 (MQ=255) cagcgcctgggaggtgttcggcgggagagtttcagcgcctgggagatcgagggccgcccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGggcatgt < 5:52476‑M2/86‑4 (MQ=255) cagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGgggcatgtc > 7:215317‑M2/63‑145 (MQ=255) tGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCt > 3:77571/1‑149 (MQ=255) agcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGgggcatgtc < 8:215317‑M2/87‑5 (MQ=255) aGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTc > 7:72836/1‑148 (MQ=255) aGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTc > 3:48643/1‑148 (MQ=255) ggCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGa < 4:76036/149‑1 (MQ=255) ggCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGACAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGa > 2:185356/1‑149 (MQ=255) gaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATcgc < 5:95135‑M2/97‑1 (MQ=255) gggccatcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATcgcg < 4:33330‑M2/98‑1 (MQ=255) gtgttcggcgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGcgtc < 6:102326‑M2/100‑1 (MQ=255) gtgttcgccgggagagtttcagcgcctgggagagcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGcgt < 1:263808‑M2/99‑1 (MQ=255) gcgcGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtc < 4:77571/149‑1 (MQ=255) cgcgTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtcg > 3:258922/1‑149 (MQ=255) gcgTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGgc > 2:258125/1‑127 (MQ=255) gcgTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGgc < 1:258125/127‑1 (MQ=255) gCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTaca > 7:33643/1‑148 (MQ=255) ccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTacaaa < 7:37460‑M2/107‑1 (MQ=255) gAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTacaaaca < 8:72836/147‑1 (MQ=255) gggTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGc < 3:134500/149‑1 (MQ=255) ggTTGTAGAGCGTGTGAGATTGTGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCCCATCTCCTACGTCCTCATTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTacaaa < 1:84046/148‑1 (MQ=25) tGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTc < 6:75656/149‑1 (MQ=255) gTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTc < 4:258922/148‑1 (MQ=255) gTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATc > 4:174209/1‑123 (MQ=255) gTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgc > 1:31127/1‑149 (MQ=255) aTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGcac < 4:48643/149‑1 (MQ=255) gcgccgcccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtc < 5:121405‑M2/100‑1 (MQ=255) gcgccgcccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtc > 6:121405‑M2/13‑112 (MQ=255) gcgcCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCaggag > 5:247235/1‑149 (MQ=14) ccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAg > 4:249155‑M2/6‑116 (MQ=255) ccgcgCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAg < 3:249155‑M2/111‑1 (MQ=255) cgcgcgGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGTCTGGc > 3:3442/1‑149 (MQ=14) cgcgCGGCGATTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACTCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGc < 1:185356‑M2/145‑1 (MQ=255) gcgcTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaa < 7:103797/149‑1 (MQ=255) ttCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaa < 8:33643/149‑1 (MQ=255) ttCGCGGGTAAACCC‑‑‑GCTCCTACCT‑‑T‑TGTTTCGGGCCAGTTATTACTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTAGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaa > 6:240718/1‑149 (MQ=255) cgcgGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac < 2:53419/148‑1 (MQ=255) cgGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTa < 2:31127/149‑1 (MQ=255) aCCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCgg > 6:245598/1‑149 (MQ=255) c‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGtt < 6:72185/149‑1 (MQ=255) cTACGT‑‑T‑TGTTTCGGGCCAATTATTCCTGCGGGATTAGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑Gaaacaaac > 3:201883/1‑130 (MQ=2) gt‑‑t‑tgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcg < 8:64077‑M2/148‑7 (MQ=255) tgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGTACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagc < 8:124034‑M2/149‑11 (MQ=255) tttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGAACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGcgcc > 7:139244/1‑149 (MQ=14) tCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACACGGCGGTCGCGCGCGCCTGGCACTGGAGGGATTGGCCATAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgcc > 8:56030‑M2/1‑135 (MQ=255) gCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcg < 3:24111‑M2/149‑19 (MQ=255) gCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCgcgg > 3:104530/1‑149 (MQ=14) cTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctc < 4:15247‑M2/148‑29 (MQ=255) cTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctc < 1:124560‑M2/148‑29 (MQ=255) tgGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcga > 4:24111‑M2/1‑117 (MQ=255) | CGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCC‑‑‑GCTCCTACGT‑‑T‑TGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGA > NC_002947/5988680‑5988965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |