| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,988,889 | C→A | 33.7% | intergenic (+183/‑363) | kefB‑III → / → PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,988,889 | 0 | C | A | 33.7% | 66.6 / 31.0 | 44 | intergenic (+183/‑363) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base C (11/18); new base A (9/6); total (20/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCAC > NC_002947/5988748‑5989031 | aTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGcac < 4:48643/149‑1 (MQ=255) gcgcCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCagga‑g > 5:247235/1‑149 (MQ=14) cgcgcgGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGA‑GGGTCTGGc > 3:3442/1‑149 (MQ=14) cgcgCGGCGATTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACTCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGc < 1:185356‑M2/145‑1 (MQ=255) gcgcTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGaaa < 7:103797/149‑1 (MQ=255) ttCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGaaacaaa < 8:33643/149‑1 (MQ=255) ttCGCGGGTAAACCCGCTCCTACCTTTGTTTCGGGCCAGTTATTACTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTAGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGaaacaaa > 6:240718/1‑149 (MQ=255) cgcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGaaacaaac < 2:53419/148‑1 (MQ=255) cgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTa < 2:31127/149‑1 (MQ=255) aCCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCgg > 6:245598/1‑149 (MQ=255) cGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGtt < 6:72185/149‑1 (MQ=255) cTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTAGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGA‑GGGACTGGCC‑Gaaacaaac > 3:201883/1‑130 (MQ=2) gtttgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcg < 8:64077‑M2/148‑7 (MQ=255) tgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGTACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagc < 8:124034‑M2/149‑11 (MQ=255) tttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGA‑GGAACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGcgcc > 7:139244/1‑149 (MQ=14) tCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACACGGCGGTCGCGCGCGCCTGGCACTGGA‑GGGATTGGCCATAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgcc > 8:56030‑M2/1‑135 (MQ=255) gCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcg < 3:24111‑M2/149‑19 (MQ=255) gCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGA‑GGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCgcgg > 3:104530/1‑149 (MQ=14) cTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctc < 4:15247‑M2/148‑29 (MQ=255) cTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctc < 1:124560‑M2/148‑29 (MQ=255) tgGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcga > 4:24111‑M2/1‑117 (MQ=255) ggACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAATCAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatct < 2:191966‑M2/148‑34 (MQ=255) cgcgCACGCGAGCCCCCTGGCGGACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGATCTCATg < 3:108838/149‑1 (MQ=255) gcgcACGCGAGCCCCCTGGCGGACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGATCTCATg > 4:108838/1‑148 (MQ=255) cgcACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgcccaatctcacagg > 7:64077‑M2/1‑110 (MQ=255) cgcgACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGA‑GGGACTGGCC‑GAAACAAACGTAg > 1:160072/1‑94 (MQ=14) gAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgat > 8:157576‑M2/1‑103 (MQ=255) cccTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGATCTCATGGGCGCCCAAAAACt < 2:124560/149‑1 (MQ=255) gcgcACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGATCTCATGGGCGCCCAAAAACTATc < 5:245598/147‑1 (MQ=255) ggcaTGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGA‑GGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgagctaaccggcgctgaaaatcctccggc < 8:216066‑M2/147‑58 (MQ=255) gCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGATCTCATGGGCGCCCAAAAACTATCGCCAGGCa > 6:37680/1‑149 (MQ=255) cgtcgtACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAATCAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCa < 1:191966/149‑1 (MQ=255) gtcgtACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATc > 6:258783/1‑149 (MQ=255) tACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGATGGGATTGGCCGGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGAGCTCAGGGGCGCCCAAAACGTATCGCCAGGCACCTGGAAGCCAACCTg > 1:176540/1‑149 (MQ=21) tACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGA‑GGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgcc < 2:60117‑M2/149‑81 (MQ=255) acaaacaaGGCGGTCGCGCGCGACTGGTGCAGGA‑GGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccg < 3:23068‑M2/149‑82 (MQ=255) acaaGGCGGTCGCGCGCGACTGGTGCAGGA‑GGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtcca < 1:120071‑M2/149‑86 (MQ=255) acaaGGCGGTCGCGCGCGACTGGTGCAGGA‑GGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattcca < 7:247467‑M2/139‑76 (MQ=255) acaaGGCGGTCGCGCGCGACTGGTGCAGGA‑GGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattcca > 8:247467‑M2/1‑64 (MQ=255) acaaGGCGGTCGCGCGCGACTGGTGCAGGA‑GGGACTGCCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtcca < 1:8259‑M2/149‑86 (MQ=255) acaaGGCGGGCGCGCGCGACTGGTGCAGGA‑GGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacatggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtcc > 1:60117‑M2/1‑64 (MQ=255) caaGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTc < 5:258783/149‑1 (MQ=255) caaGGCGGTCGCGCGCGACTGGTGCAGGA‑GGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccac > 4:23068‑M2/1‑63 (MQ=255) tCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGATCTCATGGGCGCCCAAAAACTATCGCCAGGCCCCTGGAAGCCATCCTGCAATTTCCTGACGAt > 3:147877/1‑149 (MQ=255) gcgcgcgcCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTc < 7:157536/139‑1 (MQ=255) gcgcgcgcCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTc > 8:157536/1‑139 (MQ=255) cgcgcgaCTGGTGCAGGA‑GGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattcca < 1:96433‑M2/149‑98 (MQ=255) | ATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGA‑GGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCT‑CGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCAC > NC_002947/5988748‑5989031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |