Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,027,468 A→G 7.2% V130A (GTG→GCG)  PP_5277 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,027,4680AG 7.2% 157.3 / 2.1 55V130A (GTG→GCG) PP_5277MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (23/28);  new base G (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

CGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGA  >  NC_002947/6027320‑6027613
                                                                                                                                                    |                                                                                                                                                 
cgcTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGcca                                                                                                                                                   <  1:100123/149‑1 (MQ=255)
     caccacGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAAc                                                                                                                                              >  6:100431/1‑149 (MQ=255)
      accacGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAAcc                                                                                                                                             <  4:219322/149‑1 (MQ=255)
       ccacGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCg                                                                                                                                            >  1:146118/1‑149 (MQ=255)
        cacGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCgg                                                                                                                                           >  8:71585/1‑149 (MQ=255)
                     aaCCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGccacc                                                                                                                                                 >  8:92770/1‑130 (MQ=255)
                     aaCCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGccacc                                                                                                                                                 <  7:92770/130‑1 (MQ=255)
                       ccAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTc                                                                                                                             >  2:123515/1‑148 (MQ=255)
                         aGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGac                                                                                                                          <  4:143050/149‑1 (MQ=255)
                                 ggcCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAgtt                                                                                                                  <  2:146118/149‑1 (MQ=255)
                                    cagcagcagGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAgttggt                                                                                                               <  8:210393/149‑1 (MQ=255)
                                    cagcagcagGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAgttggt                                                                                                               <  4:138528/149‑1 (MQ=255)
                                     agcagcagGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCGCCAACCGGCCACGCCACTGTTCGACCACCAgttggtt                                                                                                              >  5:192501/1‑149 (MQ=255)
                                     agcagcagGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCGCCAACCGGCCACGCCACTGTTCGACCACCAgttggtt                                                                                                              >  3:80626/1‑149 (MQ=255)
                                     agcagcagGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAgttggtt                                                                                                              >  6:238543/1‑149 (MQ=255)
                                     agcagcagGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAgttgg                                                                                                                >  7:144536/1‑147 (MQ=255)
                                     agcagcagGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAgttgg                                                                                                                <  8:144536/147‑1 (MQ=255)
                                             gCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAgttg                                                                                                                 <  7:177868/138‑1 (MQ=255)
                                             gCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAgttg                                                                                                                 >  8:177868/1‑138 (MQ=255)
                                                 gTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTc                                                                                                  >  6:199188/1‑149 (MQ=255)
                                                 gTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTc                                                                                                  <  7:71585/149‑1 (MQ=255)
                                                     ccgccCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGcc                                                                                               >  8:202497/1‑148 (MQ=255)
                                                      cgccCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCGCCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGa                                                                                             <  6:192501/149‑1 (MQ=255)
                                                      cgccCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCGCCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGa                                                                                             <  4:80626/149‑1 (MQ=255)
                                                      cgccCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGa                                                                                             <  5:238543/149‑1 (MQ=255)
                                                      cgccCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGa                                                                                             <  4:681/149‑1 (MQ=255)
                                                         ccAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGa                                                                                          <  1:123515/149‑1 (MQ=255)
                                                          cAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGAt                                                                                         <  8:14856/149‑1 (MQ=255)
                                                             aaGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGaa                                                                                      <  1:210610/149‑1 (MQ=255)
                                                               gCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAaca                                                                                    <  4:254357/149‑1 (MQ=255)
                                                                      tcgtcATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACg                                                                               <  1:30406/147‑1 (MQ=255)
                                                                      tcgtcATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACg                                                                               >  2:30406/1‑147 (MQ=255)
                                                                        gtcATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGt                                                                             >  7:154492/1‑147 (MQ=255)
                                                                           atcaGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTc                                                                                                                             >  4:243336/1‑96 (MQ=255)
                                                                           atcaGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTc                                                                                                                             <  3:243336/96‑1 (MQ=255)
                                                                             caGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAgaga                                                                       >  2:119754/1‑148 (MQ=255)
                                                                               gagCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGa                                                                                                            <  4:45630/109‑1 (MQ=255)
                                                                               gagCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGa                                                                                                            >  3:45630/1‑109 (MQ=255)
                                                                                agCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATg                                                                   <  5:163452/149‑1 (MQ=255)
                                                                                                cACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGATCATCAGCCgca                                                   >  8:205827/1‑149 (MQ=255)
                                                                                                   cAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGATCATCAGCCgcag                                                  >  1:129744/1‑147 (MQ=255)
                                                                                                          ccggccAACTGGCTCAGGGCGTAGCTGCTTCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGATCATCAGCCGCAGCAGCAGCCa                                         <  7:48371/149‑1 (MQ=255)
                                                                                                                 aCTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGATCATCAGCCGCAGCAGCAGCCATAACggg                                  <  7:205827/149‑1 (MQ=255)
                                                                                                                   tGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGatcat                                                           >  6:177302/1‑122 (MQ=255)
                                                                                                                   tGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGatcat                                                           <  5:177302/122‑1 (MQ=255)
                                                                                                                       tCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGa                                                                                             <  6:125659/84‑1 (MQ=255)
                                                                                                                       tCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGa                                                                                             >  5:125659/1‑84 (MQ=255)
                                                                                                                         aGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGAACATCAGCCGCAGCAGCAGCCATAACGGGTAACTAgg                          <  5:199188/149‑1 (MQ=255)
                                                                                                                            gCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCAAGCTTTCGCCGAGGATGAaca                                                                                    >  4:213054/1‑88 (MQ=255)
                                                                                                                            gCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCAAGCTTTCGCCGAGGATGAaca                                                                                    <  3:213054/88‑1 (MQ=255)
                                                                                                                                   tgctgcCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAgcgc                 >  1:27719/1‑148 (MQ=255)
                                                                                                                                    gctgcCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAgcgc                 >  7:160903/1‑147 (MQ=255)
                                                                                                                                              aGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAAt     >  6:268069/1‑149 (MQ=255)
                                                                                                                                               gcgcCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATc    <  7:202497/149‑1 (MQ=255)
                                                                                                                                               gcgcCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATc    <  5:268069/149‑1 (MQ=255)
                                                                                                                                               gcgcCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATc    >  3:67080/1‑149 (MQ=255)
                                                                                                                                                cgcCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGGTCAACACCACGGTCAGAGACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCg   <  6:156462/149‑1 (MQ=255)
                                                                                                                                                 gcCACCTAGCGGCCAGGCCACTGTTCGACCAGGAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACTGTCAGAGACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGa  <  2:27719/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTTGATCCAGCTTTCGCCGAGGATGAACACCACGGTCAGAGACATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGA  >  NC_002947/6027320‑6027613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑