| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,039,236 | C→T | 7.2% | intergenic (‑256/+38) | PP_5290 ← / ← pyrE | transposase/orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,039,236 | 0 | C | T | 7.2% | 157.6 / 2.1 | 55 | intergenic (‑256/+38) | PP_5290/pyrE | transposase/orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (26/25); new base T (2/2); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-01 | |||||||||||
AAAGGAGGAGGGGTGATTTCTCCTACGGTCTGTACTGCGCGAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGAAACAATGCTGAC > NC_002947/6039090‑6039381 | aaaGGAGGAGGGGTGATTTCTCCTACGGTCTGTACTGCGCGAACAGTCAGAAGCGGAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCct > 1:74111/1‑149 (MQ=255) aggaggaggGGTGATTTCTCCTACGGTCTGTACTGCGCGAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCctct > 6:185066/1‑148 (MQ=255) gaggaggGGTGATTTCTCCTACGGTATGTACTGCGCGAACAGTCAGAAGC‑GAGTTAAGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTgaa < 5:86572/149‑1 (MQ=255) gTGATTTCTCCTACGGTCTGTACTGCGCGAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAACCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCCACACACAGAAGAAGGACg > 4:32185/1‑149 (MQ=17) cTACGGTCTGTACTGCGCGAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCa < 2:74111/148‑1 (MQ=255) ggTCTGTACTGCGCGAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTGTCATTCCt > 4:150755/1‑149 (MQ=255) cTGTACTGCGCGAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTTCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTggg > 2:90166/1‑149 (MQ=255) tACTGCGCGAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTggg < 3:31674/146‑1 (MQ=255) tACTGCGCGAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCa < 3:115995/149‑1 (MQ=255) cTGCGCGAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGATGAAGGGCGCCTTTTTCATTCCTGGGCCAgg > 2:219694/1‑149 (MQ=255) cTGCGCGAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAgg < 6:31405/149‑1 (MQ=255) gAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATc > 6:89832/1‑146 (MQ=255) gAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATc < 5:89832/146‑1 (MQ=255) gAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGa < 2:58704/149‑1 (MQ=255) cAGGCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCTAGTCCTCACATACAAGCGGGTTTACCCGCGAAAGGGCCTGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATcc < 3:32185/149‑1 (MQ=37) aGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATccc > 7:179795/1‑149 (MQ=255) gAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGAGCCGCTGCAGGAGGGCGCCGTTTTGCTTACGGGGGCAGGCTGAGATGCCGTaag > 4:153542/1‑147 (MQ=18) gAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACt > 1:188774/1‑149 (MQ=255) aGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCa > 6:20420/1‑132 (MQ=255) aGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCa < 5:20420/132‑1 (MQ=255) cctcctcctCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCt > 2:56645/1‑149 (MQ=255) cctcctcctCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCt > 8:79170/1‑149 (MQ=255) ctccCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGc > 4:72513/1‑149 (MQ=255) tccCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGcc > 3:176266/1‑149 (MQ=255) cccTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCg < 7:79170/149‑1 (MQ=255) ccTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGTGCCCGGTAGGCCTCGACAGcctg > 4:151791/1‑147 (MQ=255) ccTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGTGCCCGGTAGGCCTCGACAGACgt > 8:98197/1‑148 (MQ=255) cTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCgg > 8:15636/1‑148 (MQ=255) cAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCgg > 6:266743/1‑145 (MQ=255) cAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCgg < 5:266743/145‑1 (MQ=255) tCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCt > 2:181248/1‑149 (MQ=255) cTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGt < 5:185066/149‑1 (MQ=255) aCCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGttt > 3:148949/1‑149 (MQ=255) tACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCa < 1:135263/97‑1 (MQ=255) tACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCa > 2:135263/1‑97 (MQ=255) tACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGc < 1:234582/149‑1 (MQ=255) gCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCtgtg > 2:30405/1‑148 (MQ=255) ggTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATc < 3:72513/149‑1 (MQ=255) gTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGttt < 5:122477/136‑1 (MQ=255) gTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGttt > 6:122477/1‑136 (MQ=255) aCCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAg > 4:91877/1‑149 (MQ=255) cccGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGc < 1:181248/149‑1 (MQ=255) cccGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGc < 8:179795/149‑1 (MQ=255) gcgAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAg > 3:19217/1‑149 (MQ=255) ggCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTcc < 4:176266/149‑1 (MQ=255) ccAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGATTGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAg < 1:90166/149‑1 (MQ=255) ccAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAg < 4:19217/149‑1 (MQ=255) aTCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCAcc > 5:105021/1‑149 (MQ=255) aGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTgg < 4:148949/149‑1 (MQ=255) aaaaaaGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCATGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGaaa < 3:150755/149‑1 (MQ=255) aaaaGGCGACCTTCTTCAGAGGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCt < 4:61713/105‑1 (MQ=38) aaaaGGCGACCTTCTTCAGAGGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCt > 3:61713/1‑105 (MQ=38) aaaaGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTACTGGGCCAGGATCAGATCCCGTACTGCGCCCCGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCTTCAGCCAGGAACTCCAGCACCTGGGTCAGCGaaaa > 4:190438/1‑147 (MQ=255) aaGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGAAACAAt < 7:15636/149‑1 (MQ=255) ggCGACCTTCTTCAGAGGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCa < 1:4494/86‑1 (MQ=38) ggCGACCTTCTTCAGAGGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCa > 2:4494/1‑86 (MQ=38) aCCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGAAACAATGCTGac < 1:30405/149‑1 (MQ=255) ccTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTc > 7:54777/1‑117 (MQ=255) ccTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTc < 8:54777/117‑1 (MQ=255) | AAAGGAGGAGGGGTGATTTCTCCTACGGTCTGTACTGCGCGAACAGTCAGAAGC‑GAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGAAACAATGCTGAC > NC_002947/6039090‑6039381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |