Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,051,669 C→G 14.6% intergenic (+18/‑39) ridA → / → PP_5304 aminoacrylate/iminopropionate hydrolase/deaminase/lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,051,6690CG14.6% 136.8 / 16.1 55intergenic (+18/‑39)ridA/PP_5304aminoacrylate/iminopropionate hydrolase/deaminase/lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (25/22);  new base G (4/4);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

CGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCATCACT  >  NC_002947/6051525‑6051815
                                                                                                                                                |                                                                                                                                                  
cgcgAAGGTAAACGTGGTGATGGGCCGCTACTTCTAACAGCCCTACCCGTCCCGCGCCGCCATCTGCGTTGCCGCACTGCAGAGAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGc                                                                                                                                                 <  5:205533/148‑1 (MQ=255)
   cAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCAGGGCCCGt                                                                                                                                             >  1:131762/1‑149 (MQ=255)
   cAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCAGGGCCCGt                                                                                                                                             >  5:90102/1‑149 (MQ=255)
   cAAGGTAAACGACGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCACGTCGAAATCGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACCGGCCCTGCCCGt                                                                                                                                             >  3:25103/1‑149 (MQ=255)
     aGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCAGGGCCCGTCa                                                                                                                                           <  6:90102/149‑1 (MQ=255)
     aGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCAGGGCCCGTCa                                                                                                                                           <  2:131762/149‑1 (MQ=255)
      ggTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCAc                                                                                                                                          <  1:116362/149‑1 (MQ=255)
       gTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCAcc                                                                                                                                         >  1:211038/1‑149 (MQ=255)
        tAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCAGGGCCCGTCAcca                                                                                                                                        <  4:247942/149‑2 (MQ=255)
                gTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCtccttcct                                                                                                                                >  5:235814/1‑149 (MQ=255)
                  gATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCCCTGCCGAAAGGCGCCCACGTCGAAATCCACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTGACCAGCCTTCctcg                                                                                                                              >  1:263373/1‑148 (MQ=255)
                       gCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGc                                                                                                                            <  1:130067/146‑1 (MQ=255)
                       gCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAg                                                                                                                         <  6:235814/149‑1 (MQ=255)
                            tACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTAc                                                                                                                    >  5:124981/1‑149 (MQ=255)
                             aCTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGCCGGGCCCTGCCCGTCGCCATGCTTACTATGCAAGGTTAcc                                                                                                                   >  1:98176/1‑149 (MQ=255)
                               ttCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGc                                                                                                                                                 <  6:186460/117‑1 (MQ=255)
                               ttCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGc                                                                                                                                                 >  5:186460/1‑117 (MQ=255)
                               ttCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTAcccc                                                                                                                 >  6:227788/1‑149 (MQ=255)
                                          cTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTc                                                                                                       >  6:170522/1‑148 (MQ=255)
                                               cGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCAc                                                                                                 >  6:252084/1‑149 (MQ=255)
                                                       gccgccATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTc                                                                                         >  7:145560/1‑149 (MQ=255)
                                                           ccATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCgcgc                                                                                     <  5:227788/149‑1 (MQ=255)
                                                             aTCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTg                                                                                   <  5:250099/149‑1 (MQ=255)
                                                                 gCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAg                                                                               >  4:257832/1‑149 (MQ=255)
                                                                 gCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAg                                                                               >  8:62391/1‑149 (MQ=255)
                                                                      gCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTg                                                                          >  2:178444/1‑149 (MQ=255)
                                                                               ccGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGggc                                                                 <  6:13357/149‑1 (MQ=255)
                                                                               ccGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGggc                                                                 <  8:145560/149‑1 (MQ=255)
                                                                                      gcgcCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCa                                                          >  7:33355/1‑149 (MQ=255)
                                                                                       cgcCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCAc                                                         <  1:178444/149‑1 (MQ=255)
                                                                                          ccAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAg                                                      >  1:56743/1‑149 (MQ=255)
                                                                                              gTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCca                                                   >  1:171127/1‑148 (MQ=255)
                                                                                              gTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCca                                                   >  7:224916/1‑148 (MQ=255)
                                                                                                 gAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACaaa                                               >  6:256664/1‑149 (MQ=255)
                                                                                                 gAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACaaa                                               <  5:170522/149‑1 (MQ=255)
                                                                                                  aaaTGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAAc                                              >  3:62186/1‑149 (MQ=255)
                                                                                                  aaaTGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGAAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAAc                                              >  2:140689/1‑149 (MQ=255)
                                                                                                   aaTGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGGTACCCCGTCATGCGTCAAGCACTGCCCGTCGCGCGGGCAGCCCTGCTACTGGGCGGCTGCACCCGCCACAAccc                                             >  7:88343/1‑149 (MQ=255)
                                                                                                       gACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCg                                           >  3:143739/1‑147 (MQ=255)
                                                                                                       gACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGaa                                         <  2:56743/149‑1 (MQ=255)
                                                                                                       gACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGaa                                         <  4:62186/149‑1 (MQ=255)
                                                                                                         cGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGa                                       <  6:124981/149‑1 (MQ=255)
                                                                                                         cGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGa                                       <  4:125563/149‑1 (MQ=255)
                                                                                                                      aTCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGaa                                         <  5:89416/134‑1 (MQ=255)
                                                                                                                      aTCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGaa                                         >  6:89416/1‑134 (MQ=255)
                                                                                                                       tCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCt                         <  1:140689/149‑1 (MQ=255)
                                                                                                                         gAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTg                        >  1:192685/1‑148 (MQ=255)
                                                                                                                           atgatgCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGa                      <  1:90627/148‑1 (MQ=255)
                                                                                                                              atgCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCa                   <  2:171127/148‑1 (MQ=255)
                                                                                                                               tgCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAAc                 <  8:224916/149‑1 (MQ=255)
                                                                                                                                 cccTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCTCCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCATcc                <  5:252084/148‑1 (MQ=255)
                                                                                                                                     ggTGACGGGCAGGGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACg                               >  6:228911/1‑129 (MQ=255)
                                                                                                                                     ggTGACGGGCAGGGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACg                               <  5:228911/129‑1 (MQ=255)
                                                                                                                                      gTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGaa           <  2:192685/148‑1 (MQ=255)
                                                                                                                                      gTGACGGGCAGGGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAg          >  2:234063/1‑149 (MQ=255)
                                                                                                                                              cccTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCATCACt  >  3:147817/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGCCAAGGTAAACGAGGTGATGGGCCGCTACTTCGAACAGCCCTACCCGGCCCGCGCCGCCATCGGCGTTGCCGCACTGCCGAAAGGCGCCCAGGTCGAAATGGACGCCATCCTGGTCATCGAATGATGCCCTGGTGACGGGCCCTGCCCGTCACCCTCCTTCCTCTGCAAGGTTACCCCGTCATGCGTCAAGCACTGCCCTTCGCGCTGGCAGCCCTGCTACTGGGCGGCTGCACCAGCCACAAACCCGAAGATTTCAACGGCATCTGGATCAACCAGGAAGCCATCACT  >  NC_002947/6051525‑6051815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑