| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,063,464 | T→C | 10.9% | L118P (CTG→CCG) ‡ | ubiC → | chorismate pyruvate‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,063,464 | 0 | T | C | 10.9% | 115.7 / 5.7 | 46 | L118P (CTG→CCG) ‡ | ubiC | chorismate pyruvate‑lyase |
| Reads supporting (aligned to +/- strand): ref base T (17/24); new base C (2/3); total (19/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01 | |||||||||||
GGCATCGCTGCCGGCGCCGAAGGCTGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGCACATGCGGCGCTTTGGGGCCGCCGCTCGCGCTT > NC_002947/6063319‑6063587 | ggCATCGCTGCCGGCGCCGAAGGCTGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTgg‑g < 5:52572/149‑1 (MQ=255) cTGCCGGCGCCGAAGGCTGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCCCA‑GCGAgcgg < 5:97116/149‑3 (MQ=255) ccGGCGCCGAAGGCTGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGctgctg > 5:84301/1‑149 (MQ=255) ccGGCGCCGAAGGCTGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGctgctg > 8:148184/1‑149 (MQ=255) gcCGAAGGCTGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGAATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGACAGCAGCTCGCCCA‑GCGAGcggctgccc > 8:212297/1‑144 (MQ=255) ccGAAGGCTGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGc < 1:17592/149‑1 (MQ=255) ccGAAGGCTGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGc > 3:151479/1‑149 (MQ=255) ccGAAGGCTGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGAATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGACAGCAGCTCGCCCA‑GCGAGcggctgcccaag < 7:212297/149‑7 (MQ=255) cGAAGGCTGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGc > 2:202405/1‑148 (MQ=255) gAAGGCTGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGa < 5:150977/149‑1 (MQ=255) tGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGc > 5:121737/1‑149 (MQ=255) gggTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCg > 8:89305/1‑149 (MQ=255) tGCGCGAAGTGTATCTGCGCGGCCATGGCCAAGCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCAGCTGTTCTGCGACCAGGCGTTc < 7:201397/149‑1 (MQ=255) cgcgAAGTGTATCTGTGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCAt < 7:28403/149‑1 (MQ=255) gAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCg < 6:95938/149‑1 (MQ=255) aaGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGGGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGt < 1:232622/149‑1 (MQ=255) gtATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATc < 6:267399/149‑1 (MQ=255) cgcgGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGa < 5:94169/149‑1 (MQ=255) cgGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAg < 2:168622/149‑1 (MQ=255) cATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGGATCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAgtgt < 1:202405/149‑1 (MQ=255) tGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAAGGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCa < 7:148184/149‑1 (MQ=255) ggCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCAc > 5:255777/1‑149 (MQ=255) ttGGGTTTTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGATCGTGGTGGCGTTGACCTGGAATCCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCTTCCGTCATCCACTCGAAGTGTGCa < 1:145049/140‑1 (MQ=255) ttGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCca > 5:157688/1‑148 (MQ=255) ttGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGAGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATcaa > 5:30482/1‑146 (MQ=255) gggTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCcaca < 6:84301/148‑1 (MQ=255) ggTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAg < 4:151479/148‑1 (MQ=255) cccGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCAGCTCGCCCA‑GCGAGcggctgc > 1:161677/1‑86 (MQ=38) cccGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCAGCTCGCCCA‑GCGAGcggctgc < 2:161677/87‑2 (MQ=38) gCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGc > 7:220874/1‑149 (MQ=255) aGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGc < 7:89305/149‑1 (MQ=255) gCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGca < 6:121737/149‑1 (MQ=255) cGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGcaca < 5:33963/149‑1 (MQ=255) gCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTg < 5:234335/144‑1 (MQ=255) gCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTg > 6:234335/1‑144 (MQ=255) gcCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGCACATgcggc > 1:1231/1‑149 (MQ=255) gcCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGCACATgcggc > 8:75251/1‑149 (MQ=255) aCGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGCACATGCGGCGCTTTggg > 8:83505/1‑149 (MQ=255) gtggCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGCACATGCGGCGCTTTGGGgccgc > 5:1309/1‑149 (MQ=255) ctggacctggaAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGCACATGCGGCGCTTTGGGGCCGCCGCTc < 6:255777/149‑1 (MQ=255) gacctggaAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGCACATGCGGCGCTTTGGGGCCGCCGCTcgc < 2:1231/148‑1 (MQ=255) acctggaAAGCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGCACATGCGGCGCTTTGGGGCCGCCGCTCgcgc < 4:17204/149‑1 (MQ=255) acctggaAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGCACATGCGGCGCTTTGGGGCCGCCGCTCgcgc < 8:220874/149‑1 (MQ=255) ctggaAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGCACATGCGGCGCTTTGGGGCCGCCGCTCGCGCtt > 3:203830/1‑149 (MQ=255) ggaAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGaa > 6:72645/1‑109 (MQ=255) ggaAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGaa < 5:72645/109‑1 (MQ=255) | GGCATCGCTGCCGGCGCCGAAGGCTGGGTGCGCGAAGTGTATCTGCGCGGCCATGGCCAACCTTGGGTATTCGCCCGCAGCGTGGCCAGCCGCAGCGCCCTGGAACGTGGTGGCCTGGACCTGGAAACCTTGGGCAGCCGCTCGCTGG‑GCGAGCTGCTGTTCTGCGACCAGGCGTTCATCCGTCATCCACTCGAAGTGTGCACTTATCCACAGGCCTGGCTGCCGTCCGAAGCTGCACATGCGGCGCTTTGGGGCCGCCGCTCGCGCTT > NC_002947/6063319‑6063587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |