Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,064,072 C→T 8.7% A135V (GCG→GTG)  ubiA → 4‑hydroxybenzoate octaprenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,064,0720CT 8.7% 126.6 / 2.9 46A135V (GCG→GTG) ubiA4‑hydroxybenzoate octaprenyltransferase
Reads supporting (aligned to +/- strand):  ref base C (19/23);  new base T (2/2);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

ACAGCAGACCGCCCCTTGGCCAGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTGCCGGCCGGCGCCTGGCTGCTGTATATCGCCAACCTGCT  >  NC_002947/6063927‑6064219
                                                                                                                                                 |                                                                                                                                                   
acaGCAGACCGCCCCTTGGCCAGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGtt                                                                                                                                                  >  8:228943/1‑149 (MQ=255)
  agcagACCGCCCCTTGGCCAGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCt                                                                                                                                                <  2:117357/149‑1 (MQ=255)
  agcagACCGCCCCTTGGCCAGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCt                                                                                                                                                >  5:186699/1‑149 (MQ=255)
     agACCGCCCCTTGGCCAGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGGTCGGGGCGGTGGCCTTGGCGTTCTGCt                                                                                                                                             >  7:250159/1‑149 (MQ=255)
         cGCCCCTTGGCCAGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTAccc                                                                                                                                         >  6:193946/1‑149 (MQ=255)
             ccTTGGCCAGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTc                                                                                                                                     <  8:148705/149‑1 (MQ=255)
              cTTGGCCAGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCa                                                                                                                                    <  7:228943/149‑1 (MQ=255)
              cTTGGCCAGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCa                                                                                                                                    <  2:169577/149‑1 (MQ=255)
              cTTGGCCAGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCa                                                                                                                                    <  5:157664/149‑1 (MQ=255)
                   ccAGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAg                                                                                                                               <  5:193946/149‑1 (MQ=255)
                     aGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGGGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAgcg                                                                                                                             <  3:243625/149‑1 (MQ=255)
                        ggCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTa                                                                                                                          <  4:128806/149‑1 (MQ=255)
                           cGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATTAAGCGCTacac                                                                                                                       <  6:126372/149‑1 (MQ=255)
                                  ggCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCtactac                                                                                                                <  5:95614/149‑1 (MQ=255)
                                     cACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGTGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCg                                                                                                             >  1:116023/1‑149 (MQ=255)
                                     cACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGTGTTATGCTACCCGTTCATGAAGCGCTACACCTACTACCCg                                                                                                             >  1:120055/1‑149 (MQ=255)
                                         cgAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAgg                                                                                                         <  8:49303/149‑1 (MQ=255)
                                             gcgcTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAggtggt                                                                                                     >  4:27038/1‑149 (MQ=255)
                                                         ttCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGggcggc                                                                                         >  1:91413/1‑149 (MQ=255)
                                                           cgcgATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTAcc                                                                                                               >  5:127703/1‑125 (MQ=255)
                                                           cgcgATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTAcc                                                                                                               <  6:127703/125‑1 (MQ=255)
                                                            gcgATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTa                                                                                      >  6:40701/1‑149 (MQ=255)
                                                                      tCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTgggg                                                                             >  8:140204/1‑148 (MQ=255)
                                                                           gTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTAACCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTcc                                                                       >  4:222259/1‑149 (MQ=255)
                                                                                     tgctgGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCttt                                                              >  3:101855/1‑148 (MQ=255)
                                                                                        tgGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTcaca                                                          <  3:222259/149‑1 (MQ=255)
                                                                                        tgGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTca                                                            >  4:38112/1‑147 (MQ=255)
                                                                                                tgCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTgg                                                  <  2:91413/149‑1 (MQ=255)
                                                                                                  cACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCg                                                >  8:3776/1‑149 (MQ=255)
                                                                                                  cACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCg                                                <  3:27038/149‑1 (MQ=255)
                                                                                                       aCAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACCGCCAGCGGCTGTGaa                                           <  7:140204/149‑1 (MQ=255)
                                                                                                           ccGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTgc                                       <  5:40701/149‑1 (MQ=255)
                                                                                                             gCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGTGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACCGCCAGCGGCTGTGaaagccat                                     <  2:120055/149‑7 (MQ=255)
                                                                                                             gCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGTGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACCGCCAGCGGCTGTGaaagccat                                     <  2:116023/149‑7 (MQ=255)
                                                                                                                   tCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTGCcggccggc                               >  7:132528/1‑149 (MQ=255)
                                                                                                                   tCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTGCcggccggc                               <  8:132528/149‑1 (MQ=255)
                                                                                                                     tGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTGCCGGCCGgcgc                             >  1:234402/1‑149 (MQ=255)
                                                                                                                       gCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCa                                                                     >  6:88737/1‑107 (MQ=255)
                                                                                                                       gCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCa                                                                     <  5:88737/107‑1 (MQ=255)
                                                                                                                         ggtcggtcgGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTGCCGGCCGGCGCCTgg                         <  8:250159/148‑1 (MQ=255)
                                                                                                                           tCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTGCCGGCCGGCGCCTgg                         <  7:3776/147‑1 (MQ=255)
                                                                                                                             gTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTGCCGGCCGGCGCCTgg                         <  6:160041/145‑1 (MQ=255)
                                                                                                                             gTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTGCCGGCCGGCGCCTgg                         >  5:160041/1‑145 (MQ=255)
                                                                                                                             gTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTGCCGGCCGGCGCCTGgctg                      <  2:52697/148‑1 (MQ=255)
                                                                                                                                      ggTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTGCCGGCCGGCGCCTGGCTGCTGTATATCg            >  1:128628/1‑149 (MQ=255)
                                                                                                                                                gCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTGCCGGCCGGCGCCTGGCTGCTGTATATCGCCAACctgct  >  5:209319/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ACAGCAGACCGCCCCTTGGCCAGCGGCCGGGTGCGGCCACGCGAGGCGCTGACACTGTTCGCGATTCTGGTCGGGGTGAGTTTCCTGCTGGTGCTGTGCACCAACAGCCGCACTGTCTGGCTGTCGTTCGGGGCGGTGGCCTTGGCGTTCTGCTACCCGTTCATGAAGCGCTACACCTACTACCCGCAGGTGGTGCTGGGGGCGGCGTATTCGTGGGGCATTCCCATGGCTTTCACAGCCGCTGGCGGTGAACTGCCGGCCGGCGCCTGGCTGCTGTATATCGCCAACCTGCT  >  NC_002947/6063927‑6064219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑