Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,072,589 C→T 12.9% G556D (GGC→GAC)  PP_5327 ← phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,072,5890CT12.9% 75.1 / 3.4 31G556D (GGC→GAC) PP_5327phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (15/12);  new base T (2/2);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.99e-01

CTGCTTGTTGCGGCCCAGGGCAGACATGTCGATGCCATGGGTGTGGGAATCTTGCTGCATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGAGGGTCGCGATCACCAGCACCAGCAGCAGCGCAGTGGCATAAACCAGCGGCCGCGCGGCTTCGACGTTCGGGCTCTG  >  NC_002947/6072453‑6072725
                                                                                                                                        |                                                                                                                                        
ctGCTTGTTGCGGCCCAGGGCAGACATGTCGATGCCATGGGTGTAGGAATCTTGCTGCATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCt                                                                                                                              >  1:55261/1‑149 (MQ=255)
 tGCTTGTTGCGGCCCAGGGCAGACATGTCGATGCCAGGGGTGTGGGAATCTTGCTGCATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGcc                                                                                                                               <  6:135921/147‑1 (MQ=255)
    ttgttgCGGCCCAGGGCAGACATGTCGATGCCATGGGTGTGGGAATCTTGCTGCATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTa                                                                                                                          >  3:178113/1‑149 (MQ=255)
         gCGGCCCAGGGCAGACATGTCGATGCCATGGGTGTGGGAATCTTGCTGCATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTtct                                                                                                                     >  8:149490/1‑149 (MQ=255)
           ggCCCAGGGCAGACATGTCGATGCCATGGGTGTGGGAATCTTGCTGCATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTcg                                                                                                                   >  5:270347/1‑149 (MQ=255)
              ccAGGGCAGACATGTCGATGCCATGGGTGTGGGAATCTTGCTGCATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTcgcg                                                                                                                 >  3:239534/1‑148 (MQ=255)
                 gggCAGACATGTCGATGCCATGGGTGTGGGAATCTTGCTGCATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAgg                                                                                                             <  4:178113/149‑1 (MQ=255)
                 gggAAGAAATGTGGATGCCTTGGTTGGGGTAAGCTTGCTGCAGGGTCAACTCCATACGCTTTCAATGCGTTTAAACGGCCCGCCGGGCTTAGGGGGGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAgg                                                                                                             <  3:240968/149‑1 (MQ=255)
                  ggCAGACATGTCGATGCCATGGGTGTGGGAATCTTGCTGCATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAggt                                                                                                            >  5:45627/1‑149 (MQ=255)
                        attGTCTATGCAAGGGGTTGGGCAATCTTGCTGCTTGGTCACCTCCATACGCAATCACGTCGTTTCAAAGCGCCCCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGc                                                                                                      <  4:190326/147‑1 (MQ=255)
                          tGTCGATGCCATGGGTGTGGGAATCTTGCTGCATGGTCAACTCCATACGCTATCAAGTCGTTTCAATGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGa                                                                                                    <  7:149490/149‑1 (MQ=255)
                                  ccttGGGTGTGGCAAGCTTGCTCCATGGTCAACTCCATACGCTATCACGTCGTTTCACCGGGCCGCCCGGCTTAGGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCacac                                                                                            <  1:105009/146‑1 (MQ=255)
                                             ggAATCTTGCTGCATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAg                                                                                                              >  7:163811/1‑120 (MQ=255)
                                                ttCTTCCTGCATGGGCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGa                                                                              <  3:93496/148‑1 (MQ=255)
                                                aTCTTGCTGCATGGGCAACCCCATACGCTATCATGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGa                                                                              <  8:121458/149‑1 (MQ=255)
                                                     gctgcATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGAg                                                                             <  4:239534/145‑1 (MQ=255)
                                                      ctgcATGGTCAACTCCATACGCTATCAAGTCGTTTCAAGGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGc                                                                                                      >  2:79112/1‑119 (MQ=255)
                                                         cATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGAGGGTCGCGa                                                                     >  5:29405/1‑149 (MQ=255)
                                                          aTGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAggt                                                                                                            >  5:8435/1‑109 (MQ=255)
                                                             gTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGAGGGTCGCGATcac                                                                 <  2:55261/149‑1 (MQ=255)
                                                                   tCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGAGGGTCGCGATcaccagcac                                                           >  7:98910/1‑149 (MQ=255)
                                                                     cATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGAGGGTCGCGATcaccagcac                                                           >  7:21246/1‑147 (MQ=255)
                                                                          gCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGa                                                                              >  8:24496/1‑123 (MQ=255)
                                                                             ataaaGTGGTTTCAATGGGCCGCCGGGCTTAGGGGCGGCACGCTTGCAATCAGGGTCAGTCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCacac                                                                                            <  6:16162/103‑1 (MQ=255)
                                                                             aTCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGTCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCacac                                                                                            >  5:253188/1‑106 (MQ=255)
                                                                             aTCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGTCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCacac                                                                                            >  5:16162/1‑106 (MQ=255)
                                                                             aTCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGTCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCacac                                                                                            <  6:253188/106‑1 (MQ=255)
                                                                                     gTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGAGGGTCGCGATCACCAGCACCAGCAGCAGCGCAGTgg                                          >  1:145084/1‑148 (MQ=255)
                                                                                                                    aCGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGAGGGTCGCGATCACCAGCACCAGCAGCAGCGCAGTGGCATAAACCAGCGGCCGCGCGGCTTCGACGTTc          <  4:59273/149‑1 (MQ=255)
                                                                                                                    aCGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGAGGGTCGCGATCACCAGCACCAGCAGCAGCGCAGTGGCATAAACCAGCGGCCGCGCGGCTTCGACGTTc          <  8:98910/149‑1 (MQ=255)
                                                                                                                            aaTCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGAGGGTCGCGATCACCAGCACCAGCAGCAGCGCAGTGGCATAAACCAGCGGCCGCGCGGCTTCGACGTTCGGGCTCTg  >  4:96321/1‑149 (MQ=255)
                                                                                                                                        |                                                                                                                                        
CTGCTTGTTGCGGCCCAGGGCAGACATGTCGATGCCATGGGTGTGGGAATCTTGCTGCATGGTCAACTCCATACGCTATCAAGTCGTTTCAAAGGGCCGCCCGGCTTACGGGCGGCACGCTTGCAATCAGGGTCAGCCGTCCAGCGCCTTGTACTTCTCGCGCAGGTGGTTGCGAATCCACACGGCAGAGAGGTTGAGGGTCGCGATCACCAGCACCAGCAGCAGCGCAGTGGCATAAACCAGCGGCCGCGCGGCTTCGACGTTCGGGCTCTG  >  NC_002947/6072453‑6072725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑