Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,075,747 G→C 9.6% A272G (GCC→GGC)  PP_5328 ← phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,075,7470GC 9.6% 144.2 / 7.3 52A272G (GCC→GGC) PP_5328phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (22/25);  new base C (2/3);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-01

CTGGGCCAGCCCCCCCTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCAC  >  NC_002947/6075599‑6075895
                                                                                                                                                    |                                                                                                                                                    
cTGGGCCAGCCCCCCCTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTgg                                                                                                                                                      <  5:198097/149‑1 (MQ=255)
      cAGCCCCCCCTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGc                                                                                                                                                >  4:239005/1‑149 (MQ=255)
         cccccccTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGt                                                                                                                                             <  8:107516/149‑1 (MQ=255)
          ccccccTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGCCACGGCCgac                                                                                                                                            <  3:251601/149‑3 (MQ=255)
          ccccccTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGCCACGGCCgac                                                                                                                                            <  4:245279/149‑3 (MQ=255)
            ccccTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGa                                                                                                                                          <  1:224379/149‑1 (MQ=255)
            ccccTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGCCACGGCCgacgt                                                                                                                                          <  5:201377/149‑5 (MQ=255)
              ccTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATc                                                                                                                                        <  5:108676/149‑1 (MQ=255)
                        aCCGATGATCAGCGCAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGACGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGc                                                                                                                               >  6:106449/1‑148 (MQ=255)
                               atCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTg                                                                                                                       <  5:233512/149‑1 (MQ=255)
                                tCAGCGAAATGCCGCCAACCAGCTGGGTCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTg                                                                                                                       <  3:8146/148‑1 (MQ=255)
                                 cAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGc                                                                                                                     >  7:125928/1‑149 (MQ=255)
                                   gcgcAATGCCGCCAAGCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGt                                                                                                                   <  5:106449/149‑1 (MQ=255)
                                         tGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCg                                                                                                             <  8:42106/149‑1 (MQ=255)
                                           ccgccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGAt                                                                                                           <  3:239005/149‑1 (MQ=255)
                                            cgccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATg                                                                                                          <  2:54972/149‑1 (MQ=255)
                                            cgccAACCAGCTGGGCCGTGGCAGTAAATTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACAGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGAt                                                                                                           >  1:225310/1‑148 (MQ=255)
                                             gccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATg                                                                                                          >  3:45013/1‑148 (MQ=255)
                                                  ccAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTTATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGata                                                                                                    >  6:164711/1‑149 (MQ=255)
                                                                 cAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCg                                                                                     <  6:21032/149‑1 (MQ=255)
                                                                  aGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATAGTTCACCGtctccg                                                                                    >  3:56020/1‑147 (MQ=255)
                                                                      aTTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCAt                                                                                >  5:14045/1‑149 (MQ=255)
                                                                        ttCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCAttt                                                                              <  2:225310/149‑1 (MQ=255)
                                                                        ttCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCAttt                                                                              >  4:141303/1‑149 (MQ=255)
                                                                           ggTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCg                                                                           <  7:183883/149‑1 (MQ=255)
                                                                           ggTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCg                                                                           >  8:123281/1‑149 (MQ=255)
                                                                            gtgtCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTAGGTCATTTGCgg                                                                          >  5:253666/1‑149 (MQ=255)
                                                                               tCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCa                                                                        <  5:46276/148‑1 (MQ=255)
                                                                                           gACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGtt                                                           <  7:38238/149‑1 (MQ=255)
                                                                                           gACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGtt                                                           <  3:141303/149‑1 (MQ=255)
                                                                                           gACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGAGGCAGTTCGATGCGGtt                                                           >  1:66158/1‑149 (MQ=255)
                                                                                                cTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCt                                                      <  4:45013/149‑1 (MQ=255)
                                                                                                 ttGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTc                                                     >  3:237773/1‑149 (MQ=255)
                                                                                                 ttGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTc                                                     >  8:176620/1‑149 (MQ=255)
                                                                                                      aCGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCggtg                                                 <  8:125928/148‑1 (MQ=255)
                                                                                                      aCGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCggtg                                                 <  4:237773/148‑1 (MQ=255)
                                                                                                        ggCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCggtggt                                               >  4:174458/1‑148 (MQ=255)
                                                                                                        ggCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCggtggt                                               >  4:203854/1‑148 (MQ=255)
                                                                                                         gCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACAGTCTCGGTCATTTGCGGCAGTTCTATGAGGTTCTGCTCGGTGGTGa                                             >  8:203305/1‑149 (MQ=255)
                                                                                                            gTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACct                                          >  3:185738/1‑149 (MQ=255)
                                                                                                                aGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTc                                      <  6:14045/149‑1 (MQ=255)
                                                                                                                aGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTc                                      >  7:190626/1‑149 (MQ=255)
                                                                                                                      ttGTCGCTCAGGCTGAACACTTCGCCCGTGGCACGGCCGTTGATGACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGTCTGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGtc                                  <  4:56020/147‑1 (MQ=255)
                                                                                                                         tCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCggtg                                                 <  8:33779/129‑1 (MQ=255)
                                                                                                                         tCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCggtg                                                 >  7:33779/1‑129 (MQ=255)
                                                                                                                               aGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCGAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTc                       <  3:174565/149‑1 (MQ=255)
                                                                                                                                        tcaacGGCCGTGCCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGt              >  5:258656/5‑149 (MQ=255)
                                                                                                                                          gTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGt              >  5:187260/1‑147 (MQ=255)
                                                                                                                                          gTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTCCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGt              >  3:164418/1‑147 (MQ=255)
                                                                                                                                          aacGGCCGTGCCACGCCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAACATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGt              >  8:76667/3‑147 (MQ=255)
                                                                                                                                             ggCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGc          <  4:185738/148‑1 (MQ=255)
                                                                                                                                             ggCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCt         <  7:203305/149‑1 (MQ=255)
                                                                                                                                                 gTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTCCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGac     <  4:164418/149‑1 (MQ=255)
                                                                                                                                                    gCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCTTGCGGGTCAGCTCGaccac  <  5:164711/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGGGCCAGCCCCCCCTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCAC  >  NC_002947/6075599‑6075895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑