| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,089,217 | G→A | 10.8% | M132I (ATG→ATA) | potF‑IV → | putrescine‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,089,217 | 0 | G | A | 10.8% | 122.1 / 5.4 | 46 | M132I (ATG→ATA) | potF‑IV | putrescine‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (21/20); new base A (3/2); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
ACGTGTTCCTCGCCAAGCAGATCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGT > NC_002947/6089072‑6089365 | aCGTGTTCCTCGCCAAGCAGATCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTAc > 5:166015/1‑149 (MQ=255) ccTCGCCAAGCAGATCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCg > 8:161725/1‑149 (MQ=255) gCCAAGCAGATCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCt < 7:161725/149‑1 (MQ=255) aGATCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGc < 4:66124/149‑1 (MQ=255) gAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCaa < 6:137638/149‑1 (MQ=255) gCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAAccc > 6:201809/1‑149 (MQ=255) ccGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCg < 6:102767/149‑1 (MQ=255) cGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCgg < 1:35905/149‑1 (MQ=255) ttCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGt > 5:206916/1‑149 (MQ=255) aGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTACATATACGGCAcg < 4:257795/116‑2 (MQ=255) aGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTACATATACGGCAcg > 3:257795/1‑115 (MQ=255) agcaAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGt < 7:169889/94‑1 (MQ=255) agcaAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGt > 8:169889/1‑94 (MQ=255) aaGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACaa < 6:166015/149‑1 (MQ=255) ccGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGcc > 8:117087/1‑149 (MQ=255) aaCTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGt < 6:206916/149‑1 (MQ=255) gCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGa > 1:244470/1‑147 (MQ=255) cAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAg > 6:242065/1‑149 (MQ=255) aaCACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGc > 6:262055/1‑149 (MQ=255) cacCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTg < 6:31601/149‑1 (MQ=255) cTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCaa > 6:97939/1‑110 (MQ=255) cTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCaa < 5:97939/110‑1 (MQ=255) cTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAgg < 8:103377/112‑1 (MQ=255) cTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAgg > 7:103377/1‑112 (MQ=255) gACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGAcc < 6:15096/148‑1 (MQ=255) gACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGAc < 5:242065/147‑1 (MQ=255) ccGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGAcc > 6:18038/1‑145 (MQ=255) ccGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGAcc < 5:18038/145‑1 (MQ=255) gtcACTGATGAATCTGATCGAAGCTAAGGACCCTGGCAACTAGTATGCCGTGCCGTATATGTACGGCACCGTGCTGATCTTCTTGAACCGGGCCAAGGTGAAGGCTGCACTCGGCTAGTACGCGCTGGTGGAGAGCTGGGTCGTGATCt < 8:199327/147‑1 (MQ=255) cTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCaa < 2:244470/149‑1 (MQ=255) tgatgaAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAg > 4:211946/1‑149 (MQ=255) tgaAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGaa < 5:262055/149‑1 (MQ=255) gCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAagag > 6:25566/1‑148 (MQ=255) tGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGaa > 4:67549/1‑148 (MQ=255) cGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGcc > 4:101318/1‑142 (MQ=255) cGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGcc < 3:101318/142‑1 (MQ=255) aCCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGaa < 7:4443/128‑1 (MQ=255) aCCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGaa > 8:4443/1‑128 (MQ=255) aCCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAg > 3:145354/1‑149 (MQ=255) cccGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGc < 5:25566/149‑1 (MQ=255) aacaaGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCACTgcgg > 1:226956/1‑149 (MQ=255) caaGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTgcggcg > 1:136030/1‑149 (MQ=255) acggtgccgtACATATACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCt > 7:137867/4‑149 (MQ=255) ggtgccgtACATATACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGc > 6:94798/2‑149 (MQ=255) tgccgtACATATACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTc < 5:94798/149‑1 (MQ=255) atGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGcc > 7:90434/1‑149 (MQ=255) gTACGGCACCGTTCTGATCGGCTTCAACCCGGCCaa < 8:236870/36‑1 (MQ=255) gTACGGCACCGTGCTGATCGGCTTCAACCCGGCCaa > 7:236870/1‑36 (MQ=255) gTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGt > 2:146786/1‑149 (MQ=255) gTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGt > 1:20588/1‑149 (MQ=255) gTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGt > 1:178646/1‑149 (MQ=255) | ACGTGTTCCTCGCCAAGCAGATCGAAGCCGGGGTGTTCCAGCCGCTGGATCGCAGCAAGCTGCCGAACTGGCAACACCTGGACCCGGCACTGATGAAGCTGATCGAAGCTAACGACCCGGGCAACAAGTTTGCCGTGCCGTATATGTACGGCACCGTGCTGATCGGCTTCAACCCGGCCAAGGTCAAGGCTGCACTCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGT > NC_002947/6089072‑6089365 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |