Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,150,510	C→T	7.2%	intergenic (‑2694/‑480)	PP_5393 ← / → PP_5395	metal‑binding chaperone/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,150,510	0	C	T	7.2%	160.4 / 2.2	55	intergenic (‑2694/‑480)	PP_5393/PP_5395	metal‑binding chaperone/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base T (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

AGGCACTTTTCACTGCAGAAGCGGTATGTCCTTCCCTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGTCAGCGGACAGAGCAACAAAACTAAATAAGTGTAGTGC  >  NC_002947/6150363‑6150650                                                                                                                                                      |                                                                                                                                             aGGCACTTTTCACTGCAGAAGCGGTATGTCCTTCCCTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCa                                                                                                                                             >  6:154603/1‑149 (MQ=255)     aCTTTTCACTGCAGAAGCGGTATGTCCTTCCCTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGAt                                                                                                                                         >  5:10635/1‑149 (MQ=255)       ttttCACTGCAGAAGCGGTATGTCCTTCCCTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTc                                                                                                                                       >  8:244610/1‑149 (MQ=255)           cACTGCAGAAGCGGTATGTCCTTCCCTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGgt                                                                                                                                    <  7:159385/148‑1 (MQ=255)             cTGGAGAAGCGGTATGTCCTTCCCTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGtgtg                                                                                                                                 <  2:128169/149‑1 (MQ=255)                cAGAAGCGGTATGTCCTTCCCTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCAt                                                                                                                              <  6:10635/149‑1 (MQ=255)                cAGAAGCGGTATGTCCTTCCCTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGAATCGGTGTGCAt                                                                                                                              >  1:153142/1‑149 (MQ=255)                              ccTTCCCTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCtt                                                                                                                >  5:85542/1‑149 (MQ=255)                                ttCCCTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCAt                                                                                                                              >  5:253107/1‑133 (MQ=255)                                ttCCCTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCAt                                                                                                                              <  6:253107/133‑1 (MQ=255)                                  cccTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAACTCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTttgt                                                                                                             <  6:220686/148‑1 (MQ=255)                                             atCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGAGA‑GTGGTAA‑TGGTCGTTCTGGCACGATTCGGTGTGCATGAATTAGTTCCCTTTGTTGTCGTGTTTg                                                                                                  >  4:36021/2‑148 (MQ=255)                                              tCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGa                                                                                                >  6:265913/1‑149 (MQ=255)                                               cGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGCCACGATTCGGTGTGCATGAAGTCGTTCCCTTTGTTGTCCGGTTTGGAc                                                                                               <  7:244610/149‑1 (MQ=255)                                                gAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGa                                                                                                <  7:75367/147‑1 (MQ=255)                                                        gACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCa                                                                                      <  5:265913/149‑1 (MQ=255)                                                        gACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCa                                                                                      >  4:151400/1‑149 (MQ=255)                                                        gACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GGGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCa                                                                                      <  6:114001/149‑1 (MQ=255)                                                          cTATCCGGTTGCACCGTCATACCTAATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCAcc                                                                                    <  1:47659/149‑1 (MQ=255)                                                             tCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCtt                                                                                 >  8:206094/1‑149 (MQ=255)                                                               cGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTc                                                                                <  6:85542/148‑1 (MQ=255)                                                                    gCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGt                                                                            >  1:9598/1‑147 (MQ=255)                                                                    gCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGGTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTga                                                                          <  6:250216/149‑1 (MQ=255)                                                                      aCCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCtt                                                                                                                >  4:162116/1‑109 (MQ=255)                                                                      aCCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCtt                                                                                                                <  3:162116/109‑1 (MQ=255)                                                                          tCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTttg                                                                                                              <  1:251584/107‑1 (MQ=255)                                                                          tCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTttg                                                                                                              >  2:251584/1‑107 (MQ=255)                                                                            aTACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATAcc                                                                   <  3:151400/148‑1 (MQ=255)                                                                                cGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGt                                                                            <  6:45609/135‑1 (MQ=255)                                                                                cGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGt                                                                            >  5:45609/1‑135 (MQ=255)                                                                                  cATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATg                                                            >  1:76245/1‑149 (MQ=255)                                                                                     aCCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGAGGTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAATTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATAc                                                                    >  1:191330/1‑139 (MQ=255)                                                                                     aCCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATAc                                                                    <  2:191330/138‑1 (MQ=255)                                                                                      ccAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTtg                                                        >  6:247143/1‑149 (MQ=255)                                                                                         aaTCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGc                                                     >  6:151178/1‑149 (MQ=255)                                                                                               gcTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAag                                               <  7:206094/149‑1 (MQ=255)                                                                                               gcTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAag                                               <  5:154603/149‑1 (MQ=255)                                                                                                   aCTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCACTGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAgg                                            >  1:184121/1‑149 (MQ=255)                                                                                                   aCTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGTACGATTCGGTGTGCATGAAGTTGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGt                                           >  7:81116/1‑149 (MQ=255)                                                                                                   aCTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGTACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGt                                           >  7:85001/1‑149 (MQ=255)                                                                                                       ccATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGt                                       >  7:7492/1‑149 (MQ=255)                                                                                                       ccATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGGTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGc                                         <  4:228683/147‑1 (MQ=255)                                                                                                                 gCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGTCAGCGGACa                              <  2:184121/148‑1 (MQ=255)                                                                                                                   tCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGTCAGCGGACa                              >  3:177387/1‑146 (MQ=255)                                                                                                                      tGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGTCAGCGGACAGAGcaa                        <  5:151178/149‑1 (MQ=255)                                                                                                                                a‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGTCAGCGGACAGAGCAACAAAACTaaa              <  2:76245/149‑1 (MQ=255)                                                                                                                                   tGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGTCAGCGGACAGAGCAACAAAACTAAAt             >  4:45331/1‑148 (MQ=255)                                                                                                                                     gTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGTCAGCGGACAGAGCAACAAAACTAAATaa           <  3:36021/148‑1 (MQ=255)                                                                                                                                      tCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTACCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGTCAGCGGACAGAGCAACAAAACTAAATAAgt         >  1:134533/1‑149 (MQ=255)                                                                                                                                           ggTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGTCAGCGGACAGAGCAACAAAACTAAATAAGTGTAg     >  2:248538/1‑149 (MQ=255)                                                                                                                                               gTGCTGGTACTATTCGGTGTGCATGAAGTAGTTCACTTTTTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGTCAGCGGACAGAGCAACAAAACTAAATAAGTGTAGTGc  <  8:85001/148‑1 (MQ=255)                                                                                                                                               gTGCTGGTACGATTCGGGGTGCATGAAGTAGTTCCCTTTCTTGTGCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGGCATGGTTGTGCGCGATGAGGTGCGTCAGCGGAGAGAGCAAGAAAACTAAATTAGTGTAGTGc  <  8:81116/148‑1 (MQ=255)                                                                                                                                               gTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGTCAGCGGACAGAGCAACAAAACTAAATAAGTGTAGTGc  <  5:247143/148‑1 (MQ=255)                                                                                                                                                     gCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCg                                                  >  1:47857/1‑94 (MQ=255)                                                                                                                                                     gCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCg                                                  <  2:47857/94‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                             AGGCACTTTTCACTGCAGAAGCGGTATGTCCTTCCCTCGTATAGCTCGAAGTAGCGACTATCCGGTGGCACCGTCATACCGCATACCAAATCGCGCTGACTACCATCCGGTTGCTCATGCGGTTGCGA‑GTGGTCA‑TGGTCGTGCTGGCACGATTCGGTGTGCATGAAGTAGTTCCCTTTGTTGTCCGGTTTGGACGAGTTTTCACCCTTCTGGTGAGAATACCTGCCATGGTTGTGCCCGAAGAGGTGCGTCAGCGGACAGAGCAACAAAACTAAATAAGTGTAGTGC  >  NC_002947/6150363‑6150650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑