| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 547,203 | A→G | 12.9% | H18R (CAC→CGC) | fusA → | elongation factor G 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 547,203 | 0 | A | G | 12.9% | 134.5 / 11.1 | 54 | H18R (CAC→CGC) | fusA | elongation factor G 1 |
| Reads supporting (aligned to +/- strand): ref base A (19/28); new base G (3/4); total (22/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 | |||||||||||
AGAAGCGTGAAGACGTTCACCGTATGGCTGAAGCCAACAAAGCGTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTG > NC_002947/547056‑547349 | agaagCGTGAAGACGTTCACCGTATGGCTGAAGCCAACAAAGCGTTCTCGCACTATCGCTTCTATTTCAAGCATCAATCTTTTTGCGTGGGCTTTATGGCTCGTACTACAGGATTTTAGAGCTACAGTAACATTGTTATCTGTGCCCAc < 6:214127/149‑1 (MQ=255) aaGACGTTCACCGTATGGCTGAAGCCAACAAAGCGTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGAcgcg > 3:136704/1‑149 (MQ=255) aaGACGTTCACCGTATGGCTGAAGCCAACAAAGCGTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGAcgc < 3:119253/148‑1 (MQ=255) ttCAGCGTATGGCTGAAGCCTACAAAGCGTTCTCGCACTACCGCTTCTAATTCAAGCAGCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCGCGTCAACGTGGGCaca < 3:235865/149‑3 (MQ=255) ttCACCGTATGGCTGAAGCCAACAAAGCGTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCGCGTCAACGTGGGCac > 6:211706/1‑147 (MQ=255) ttCACCGTATGGCTGAAGCCAACAAAGCGTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCGCGTCAACGTGGGCac < 5:211706/148‑2 (MQ=255) cACCGTATGGCTGAAGCCAACAAAGCGTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTACGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGa < 6:60677/148‑1 (MQ=255) gCCAACAAAGCGTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGTCTTTATTGCTAGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTTCCCACTTTGAAGCTGGCAAGACCACAACTACCGAGCg > 6:85173/1‑149 (MQ=255) aaaGCGTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCGCGTCAACGTGGGCacag < 6:12632/127‑4 (MQ=255) aaGCGTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCGCGTCAACGTGGGCaca < 4:96535/125‑3 (MQ=255) aaGCGTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCGCGTCAACGTGGGCaca > 3:96535/1‑123 (MQ=255) gCGTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGtt < 4:122132/149‑1 (MQ=255) gTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGc > 8:84740/1‑137 (MQ=255) gTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGc < 7:84740/137‑1 (MQ=255) gTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCt < 1:32015/149‑1 (MQ=255) cACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAgg > 6:46119/1‑149 (MQ=255) aCTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGt < 7:10756/138‑1 (MQ=255) aCTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGt > 8:10756/1‑138 (MQ=255) ccGCTTCTAATTCAGGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGa < 1:130944/149‑1 (MQ=255) ttCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGc < 1:63866/96‑1 (MQ=255) ttCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGc > 2:63866/1‑96 (MQ=255) ttCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAAt > 6:10516/1‑149 (MQ=255) aaGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATgg < 8:47317/148‑1 (MQ=255) aGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGc < 2:146785/149‑1 (MQ=255) aGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGc > 8:72453/1‑149 (MQ=255) cATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGa < 7:72453/149‑1 (MQ=255) aTCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAGCATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAg > 6:177296/1‑149 (MQ=255) aTCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGtt > 8:61689/1‑118 (MQ=255) aTCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGtt < 7:61689/118‑1 (MQ=255) aTCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAg > 1:171772/1‑149 (MQ=255) tttGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CAt < 4:136704/146‑1 (MQ=255) tATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGAGAGGTG‑CATGACGGCGCCGCGacc < 5:46119/148‑1 (MQ=255) aTGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGaccac > 3:61240/1‑149 (MQ=255) tGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGaccac < 2:7819/148‑1 (MQ=255) tCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGt > 8:127579/1‑123 (MQ=255) tCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGt < 7:127579/123‑1 (MQ=255) cAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGac > 2:81378/1‑58 (MQ=255) cAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGac < 1:81378/58‑1 (MQ=255) gCAATTAACCGCTACCGTAGCATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGACTGGATGGTgca < 5:177296/149‑1 (MQ=255) aaTTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGACTGGATGGTgcagg < 5:10516/149‑1 (MQ=255) aaTTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGACTGGATGGTgcag > 1:4990/1‑148 (MQ=255) aCCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGaccacc < 7:6820/128‑1 (MQ=255) aCCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGaccacc > 8:6820/1‑128 (MQ=255) ccGTAACATTGGTATCTGTGCCCAGGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGACTGGATGGTGCAGGAGCAGGAGCggg < 5:85173/149‑1 (MQ=255) ccGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGACTGGATGGTGCAGGAGCAGGAGCggg < 2:171772/149‑1 (MQ=255) aCATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTGCCTTGACGGCGCCGCGACCACCGACTGGATGGTGCAGGAGCAGGAGCGGGGTAt < 1:202910/149‑1 (MQ=255) aTTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCAc < 4:61240/149‑1 (MQ=255) gTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCcgcg > 5:115380/1‑104 (MQ=255) gTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCcgcg < 6:115380/104‑1 (MQ=255) gTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGACTGGa < 1:267647/117‑1 (MQ=255) gTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGACTGGa > 2:267647/1‑117 (MQ=255) tcttgCCCGCGTCAACGTGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCt > 4:118971/4‑149 (MQ=25) ccACGTTGACGCGGGCAAGACCACCACTGCCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGGCTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTg > 6:107862/1‑149 (MQ=255) ccACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGACTGGATGGTgcagga < 6:147457/117‑1 (MQ=255) | AGAAGCGTGAAGACGTTCACCGTATGGCTGAAGCCAACAAAGCGTTCTCGCACTACCGCTTCTAATTCAAGCATCAATCTTTTTGCGAGGGCTTTATGGCTCGTACTACAGCAATTAACCGCTACCGTAACATTGGTATCTGTGCCCACGTTGACGCGGGCAAGACCACCACTACCGAGCGGATCCTGTTCTACACAGGTCTGAGCCACAAAATGGGCGAGGTG‑CATGACGGCGCCGCGACCACCGACTGGATGGTGCAGGAGCAGGAGCGGGGTATCACCATTACCTCCGCTG > NC_002947/547056‑547349 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |