Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 550,549 C→G 11.8% intergenic (+30/‑112) tufB → / → rpsJ elongation factor Tu‑B/30S ribosomal protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947550,5490CG11.8% 178.4 / 14.2 68intergenic (+30/‑112)tufB/rpsJelongation factor Tu‑B/30S ribosomal protein S10
Reads supporting (aligned to +/- strand):  ref base C (31/28);  new base G (6/2);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01

ATTCAGATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGG  >  NC_002947/550403‑550694
                                                                                                                                                   |                                                                                                                                                 
aTTCAGATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGGGTTATCACTCGACCGCTTGAAAAAA‑CCCCCGCTAAg                                                                                                                                                 >  8:193195/1‑149 (MQ=255)
    aGATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATAGAGTAATCACTCGACCGATTGAAAAAA‑CCACCCCTCAGCggg                                                                                                                                             >  8:160355/1‑149 (MQ=255)
     gATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCgggg                                                                                                                                            >  7:87970/1‑149 (MQ=255)
         aCTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGttt                                                                                                                                        >  8:67644/1‑149 (MQ=255)
          cTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGtttt                                                                                                                                       <  4:20638/149‑1 (MQ=255)
             ttACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGttttttt                                                                                                                                    <  5:230839/149‑1 (MQ=255)
                 ccTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTAtt                                                                                                                                <  7:67644/149‑1 (MQ=255)
                 ccTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCCATTTAAAAAA‑CCCCCGCTGAGCGGGGGttttttcaatc                                                                                                                                >  1:139958/1‑144 (MQ=255)
                    gATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAACGCGGTCGTACCGTCGGCCCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCACCGGGGGTTTTTTTTATTggg                                                                                                                             >  2:188190/1‑149 (MQ=255)
                       cAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGtt                                                                                                                           >  8:102611/1‑148 (MQ=255)
                        aaGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGa                                                                                                                         <  5:276316/149‑1 (MQ=255)
                         aGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGa                                                                                                                         >  5:159266/1‑148 (MQ=255)
                         aGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGa                                                                                                                         >  7:180315/1‑148 (MQ=255)
                            ccATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGTGGTTTTTTTTATTGGGTTGacac                                                                                                                      <  3:102968/148‑1 (MQ=255)
                             cATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGtt                                                                                                                                         >  7:171014/1‑128 (MQ=255)
                             cATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGtt                                                                                                                                         <  8:171014/128‑1 (MQ=255)
                             cATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTa                                                                                                                    <  1:159825/149‑1 (MQ=255)
                                  cAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTa                                                                                                                                  <  3:212136/130‑1 (MQ=255)
                                  cAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTa                                                                                                                                  >  4:212136/1‑130 (MQ=255)
                                  cAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTgg                                                                                                               <  7:160355/149‑1 (MQ=255)
                                      ggATGACGGGCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTTATTgggg                                                                                                             <  1:188190/148‑1 (MQ=37)
                                        aaGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGt                                                                                                         >  5:129265/1‑149 (MQ=255)
                                        aaGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGt                                                                                                         <  7:14987/149‑1 (MQ=255)
                                          gACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTGAGCGGGGGtttt                                                                                                                                       <  2:117487/117‑1 (MQ=255)
                                          gACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTGAGCGGGGGtttt                                                                                                                                       >  1:117487/1‑117 (MQ=255)
                                          gACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTGAGCGGGGGtt                                                                                                                                         >  7:112322/1‑115 (MQ=255)
                                          gACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTGAGCGGGGGtt                                                                                                                                         <  8:112322/115‑1 (MQ=255)
                                          gACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTc                                                                                                        >  3:156057/1‑148 (MQ=255)
                                            cGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCtat                                                                                                     <  8:180315/149‑1 (MQ=255)
                                                      ttCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGc                                                                                           >  1:164312/1‑149 (MQ=255)
                                                        cGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGc                                                                                           <  8:87970/147‑1 (MQ=255)
                                                          cTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGcctcc                                                                                       >  4:242356/1‑149 (MQ=255)
                                                          cTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGcctcc                                                                                       <  8:87934/149‑1 (MQ=255)
                                                               cGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCtttt                                                                                   <  6:129265/148‑1 (MQ=255)
                                                               cGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCttt                                                                                    >  3:140769/1‑147 (MQ=255)
                                                                   aaGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCgg                                                                              >  5:185361/1‑149 (MQ=255)
                                                                          tCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGt                                                                       <  6:159266/149‑1 (MQ=255)
                                                                              aCCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGt                                                                   >  1:137804/1‑149 (MQ=255)
                                                                                    ggCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGtt                                                              <  7:102611/148‑1 (MQ=255)
                                                                                          ggCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACa                                                       <  2:137804/149‑1 (MQ=255)
                                                                                          ggCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACa                                                       <  6:185361/149‑1 (MQ=255)
                                                                                           gCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACg                                                                                            <  3:47868/111‑1 (MQ=255)
                                                                                           gCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACg                                                                                            >  4:47868/1‑111 (MQ=255)
                                                                                           gCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAg                                                      >  6:277606/1‑149 (MQ=255)
                                                                                           gCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGAATGAAAAAA‑CCCCCGCTCCGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGCGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAg                                                      <  7:193195/149‑1 (MQ=255)
                                                                                              ttGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCt                                                   >  5:43485/1‑149 (MQ=255)
                                                                                                   gCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGt                                              >  8:112257/1‑149 (MQ=255)
                                                                                                    ccAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTc                                             >  7:1859/1‑149 (MQ=255)
                                                                                                      aaaaTCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCt                                            >  8:57711/1‑148 (MQ=255)
                                                                                                             tcGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGcctc                                                                                        >  8:14388/1‑97 (MQ=255)
                                                                                                             tcGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGcctc                                                                                        <  7:14388/97‑1 (MQ=255)
                                                                                                             tcGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCt                                                   >  6:20663/1‑134 (MQ=255)
                                                                                                             tcGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATcc                                     >  2:187928/1‑148 (MQ=255)
                                                                                                                aGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATg                                 <  4:156057/149‑1 (MQ=255)
                                                                                                                     tCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCaaaa                            <  5:277606/149‑1 (MQ=255)
                                                                                                                       aCTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATc                                      >  5:75403/1‑137 (MQ=255)
                                                                                                                       aCTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATc                                      <  6:75403/137‑1 (MQ=255)
                                                                                                                        cTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCa                         <  3:242356/149‑1 (MQ=255)
                                                                                                                        cTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCa                         <  6:43485/149‑1 (MQ=255)
                                                                                                                              caataaaaaaaa‑CCCCCGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGGAAAATCAGCaaag                   >  5:147111/7‑148 (MQ=255)
                                                                                                                              cGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAAt                   >  3:14520/1‑149 (MQ=255)
                                                                                                                               aataaaaaaaa‑CCCCCGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATc                  >  4:68584/6‑149 (MQ=255)
                                                                                                                                   aaaaaaa‑CCCCCGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTa              >  1:242839/2‑149 (MQ=255)
                                                                                                                                     aaaaa‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATc            >  3:219738/1‑149 (MQ=255)
                                                                                                                                     aaaaa‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATc            <  7:112257/149‑1 (MQ=255)
                                                                                                                                            ccccGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCATCAAATCCGTATCAGGtt       >  1:141899/1‑148 (MQ=255)
                                                                                                                                            ccccGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGtt       >  7:242915/1‑148 (MQ=255)
                                                                                                                                                gCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAgg  >  6:214675/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATTCAGATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAA‑CCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGG  >  NC_002947/550403‑550694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑