Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 556,986 A→C 9.3% K12T (AAG→ACG)  rpsN → 30S ribosomal protein S14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947556,9860AC 9.3% 152.3 / 7.7 54K12T (AAG→ACG) rpsN30S ribosomal protein S14
Reads supporting (aligned to +/- strand):  ref base A (27/22);  new base C (2/3);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

CGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGC  >  NC_002947/556846‑557132
                                                                                                                                            |                                                                                                                                                  
cgcgGTTTGGACATCACGCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTc                                                                                                                                               <  3:159843/146‑1 (MQ=255)
cgcgGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAg                                                                                                                                             <  1:269559/148‑1 (MQ=255)
          aCATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTCGCGGtttt                                                                                                                                  <  2:80669/149‑4 (MQ=255)
             tCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCaaa                                                                                                                               <  4:254188/149‑1 (MQ=255)
               aCCCTGACCACCACTGCTCTTTCTGACGACGAAGGCCGCGCTATGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTTGAGTAGGAAATTGGCCAAGAATATCATGTAAAACCGCGATCTTAAGCGTCAGCTCACGGTAGAAAAGt                                                                                                                             <  2:240117/149‑1 (MQ=255)
               aCCCTGACCACCACTGCTCGTTAGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACAGCGAGATGAAGCGTCAGCTCACGGTAGCaaact                                                                                                                             >  1:240117/1‑147 (MQ=255)
                     accaccACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCa                                                                                                                                              >  6:221756/1‑126 (MQ=255)
                     accaccACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCa                                                                                                                                              <  5:221756/126‑1 (MQ=255)
                      ccaccaCTGCTCGTTCTGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTa                                                                                                                       <  7:216923/148‑1 (MQ=255)
                       caccacTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCt                                                                                                                        >  1:171224/1‑146 (MQ=255)
                            cTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCg                                                                                                                <  3:209480/149‑1 (MQ=255)
                                  gTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTgct                                                                                                            >  1:1420/1‑147 (MQ=255)
                                             gaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGa                                                                                               >  3:193065/1‑149 (MQ=255)
                                                  ccGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATc                                                                                          >  6:134298/1‑149 (MQ=255)
                                                   cgcgCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGATGAAGCGTCAGCTGACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGACCTGAAAGCGACCATCg                                                                                         >  7:182594/1‑149 (MQ=255)
                                                       ctctGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCaa                                                                                     <  4:193065/149‑1 (MQ=255)
                                                              gcGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCCGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAAc                                                                              >  4:129690/1‑149 (MQ=255)
                                                               cGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTGACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTAACCCTGAACg                                                                             >  8:201137/1‑149 (MQ=255)
                                                                     ttCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCtctc                                                                       >  3:128502/1‑149 (MQ=255)
                                                                              ccGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGt                                                                                                               <  8:173882/100‑1 (MQ=255)
                                                                              ccGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGt                                                                                                               >  7:173882/1‑100 (MQ=255)
                                                                               cGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCg                                                             >  8:44926/1‑149 (MQ=255)
                                                                               cGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCg                                                             >  7:7637/1‑149 (MQ=255)
                                                                                 ttCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGtt                                                           >  2:156702/1‑149 (MQ=255)
                                                                                   ccGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGttt                                                          <  1:156702/148‑1 (MQ=255)
                                                                                     gCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGc                                                       <  4:128502/149‑1 (MQ=255)
                                                                                        aCTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGAGAGCTGAAGCGTCAGATCACGGTAGCAAAGTTCGCTAAGAAGATTGCTGAGCTGAAAGAGACCATCCTCAACATGAACGCCTCTCCAGAAGAGCGTTTCGATg                                                     >  7:224433/1‑148 (MQ=255)
                                                                                                   tAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGctc                                         >  5:201237/1‑149 (MQ=255)
                                                                                                     ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGc                                                                            <  8:100099/112‑1 (MQ=255)
                                                                                                     ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGc                                                                            >  7:100099/1‑112 (MQ=255)
                                                                                                     ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGc                                                    >  3:226231/1‑136 (MQ=255)
                                                                                                     ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGc                                                    <  4:226231/136‑1 (MQ=255)
                                                                                                     ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGctct                                        <  2:1420/148‑1 (MQ=255)
                                                                                                     ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTg                                       >  8:245873/1‑149 (MQ=255)
                                                                                                       aaaaTGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCa                                     <  2:171224/149‑1 (MQ=255)
                                                                                                         aaTGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTg                                               >  5:199851/1‑137 (MQ=255)
                                                                                                         aaTGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTg                                               <  6:199851/137‑1 (MQ=255)
                                                                                                             tccAAGAAGAGCATGAAAAACCTCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAagca                               <  5:134298/148‑1 (MQ=255)
                                                                                                               cAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAagcagc                             >  1:75132/1‑149 (MQ=255)
                                                                                                                  taagaGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGCACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAACCAGCCAc                          <  7:44926/148‑1 (MQ=255)
                                                                                                                   aagaGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCAcg                         >  7:66161/1‑149 (MQ=255)
                                                                                                                     gagCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCg                                                        <  5:85229/116‑1 (MQ=255)
                                                                                                                     gagCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCg                                                        >  6:85229/1‑116 (MQ=255)
                                                                                                                        cATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACg                    >  8:183823/1‑149 (MQ=255)
                                                                                                                                 ccGTGAGCTGACGCTTCAGCTCGCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGAGCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCACTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCt           >  2:65144/1‑149 (MQ=255)
                                                                                                                                 ccGTGAGCTGACGCTTCAGCTCGCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGttt                                                          <  1:110458/102‑1 (MQ=39)
                                                                                                                                 ccGTGAGCTGACGCTTCAGCTCGCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGttt                                                          >  2:110458/1‑102 (MQ=39)
                                                                                                                                 ccGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAg                <  2:6698/144‑1 (MQ=255)
                                                                                                                                 ccGCGAGCTGAAGCGTCAGCTCACGGGAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAg                >  1:6698/1‑144 (MQ=255)
                                                                                                                                  cgtgAGCTGACGCTTCAGCTCGCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCACTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTc          <  1:65144/146‑1 (MQ=255)
                                                                                                                                   gcgAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTcg         <  7:245873/149‑1 (MQ=255)
                                                                                                                                   gcgAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTcg         >  7:50077/1‑149 (MQ=255)
                                                                                                                                          gAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTgc  <  3:129690/149‑1 (MQ=255)
                                                                                                                                          gAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGATGAAAGCGACCATCGTCAACCTGAAATCCTCTAAAGAAGAGCGTTTAGATGCAGTTGt                                              >  5:252551/1‑105 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
CGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGC  >  NC_002947/556846‑557132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑