Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 556,997 A→G 9.4% T16A (ACG→GCG)  rpsN → 30S ribosomal protein S14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947556,9970AG 9.4% 139.0 / 4.9 53T16A (ACG→GCG) rpsN30S ribosomal protein S14
Reads supporting (aligned to +/- strand):  ref base A (28/20);  new base G (2/3);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

ACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAACCGTTGC  >  NC_002947/556856‑557143
                                                                                                                                             |                                                                                                                                                  
aCATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCTTCAGCTCGCGGtttt                                                                                                                                             <  2:80669/149‑4 (MQ=255)
   tCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCaaa                                                                                                                                          <  4:254188/149‑1 (MQ=255)
     aCCCTGACCACCACTGCTCTTTCTGACGACGAAGGCCGCGCTATGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTTGAGTAGGAAATTGGCCAAGAATATCATGTAAAACCGCGATCTTAAGCGTCAGCTCACGGTAGAAAAGt                                                                                                                                        <  2:240117/149‑1 (MQ=255)
     aCCCTGACCACCACTGCTCGTTAGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACAGCGAGATGAAGCGTCAGCTCACGGTAGCaaact                                                                                                                                        >  1:240117/1‑147 (MQ=255)
            ccaccaCTGCTCGTTCTGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTa                                                                                                                                  <  7:216923/148‑1 (MQ=255)
             caccacTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCt                                                                                                                                   >  1:171224/1‑146 (MQ=255)
                  cTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCg                                                                                                                           <  3:209480/149‑1 (MQ=255)
                        gTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTgct                                                                                                                       >  1:1420/1‑147 (MQ=255)
                                   gaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGa                                                                                                          >  3:193065/1‑149 (MQ=255)
                                        ccGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATc                                                                                                     >  6:134298/1‑149 (MQ=255)
                                         cgcgCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGATGAAGCGTCAGCTGACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGACCTGAAAGCGACCATCg                                                                                                    >  7:182594/1‑149 (MQ=255)
                                             ctctGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCaa                                                                                                <  4:193065/149‑1 (MQ=255)
                                                    gcGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCCGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAAc                                                                                         >  4:129690/1‑149 (MQ=255)
                                                     cGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTGACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTAACCCTGAACg                                                                                        >  8:201137/1‑149 (MQ=255)
                                                           ttCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCtctc                                                                                  >  3:128502/1‑149 (MQ=255)
                                                                    ccGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGt                                                                                                                          <  8:173882/100‑1 (MQ=255)
                                                                    ccGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGt                                                                                                                          >  7:173882/1‑100 (MQ=255)
                                                                     cGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCg                                                                        >  7:7637/1‑149 (MQ=255)
                                                                     cGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCg                                                                        >  8:44926/1‑149 (MQ=255)
                                                                       ttCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGtt                                                                      >  2:156702/1‑149 (MQ=255)
                                                                         ccGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGttt                                                                     <  1:156702/148‑1 (MQ=255)
                                                                           gCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGc                                                                  <  4:128502/149‑1 (MQ=255)
                                                                              aCTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGAGAGCTGAAGCGTCAGATCACGGTAGCAAAGTTCGCTAAGAAGATTGCTGAGCTGAAAGAGACCATCCTCAACATGAACGCCTCTCCAGAAGAGCGTTTCGATg                                                                >  7:224433/1‑148 (MQ=255)
                                                                                         tAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGctc                                                    >  5:201237/1‑149 (MQ=255)
                                                                                           ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGc                                                                                       <  8:100099/112‑1 (MQ=255)
                                                                                           ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGc                                                                                       >  7:100099/1‑112 (MQ=255)
                                                                                           ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGc                                                               <  4:226231/136‑1 (MQ=255)
                                                                                           ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGc                                                               >  3:226231/1‑136 (MQ=255)
                                                                                           ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGctct                                                   <  2:1420/148‑1 (MQ=255)
                                                                                           ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTg                                                  >  8:245873/1‑149 (MQ=255)
                                                                                             aaaaTGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCa                                                <  2:171224/149‑1 (MQ=255)
                                                                                               aaTGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTg                                                          <  6:199851/137‑1 (MQ=255)
                                                                                               aaTGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTg                                                          >  5:199851/1‑137 (MQ=255)
                                                                                                   tccAAGAAGAGCATGAAAAACCTCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAagca                                          <  5:134298/148‑1 (MQ=255)
                                                                                                     cAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAagcagc                                        >  1:75132/1‑149 (MQ=255)
                                                                                                        taagaGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGCACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAACCAGCCAc                                     <  7:44926/148‑1 (MQ=255)
                                                                                                         aagaGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCAcg                                    >  7:66161/1‑149 (MQ=255)
                                                                                                           gagCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCg                                                                   <  5:85229/116‑1 (MQ=255)
                                                                                                           gagCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCg                                                                   >  6:85229/1‑116 (MQ=255)
                                                                                                              cATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACg                               >  8:183823/1‑149 (MQ=255)
                                                                                                                       ccGTGAGCTGACGCTTCAGCTCGCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGAGCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCACTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCt                      >  2:65144/1‑149 (MQ=255)
                                                                                                                       ccGTGAGCTGACGCTTCAGCTCGCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGttt                                                                     >  2:110458/1‑102 (MQ=39)
                                                                                                                       ccGTGAGCTGACGCTTCAGCTCGCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGttt                                                                     <  1:110458/102‑1 (MQ=39)
                                                                                                                       ccGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAg                           <  2:6698/144‑1 (MQ=255)
                                                                                                                       ccGCGAGCTGAAGCGTCAGCTCACGGGAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAg                           >  1:6698/1‑144 (MQ=255)
                                                                                                                        cgtgAGCTGACGCTTCAGCTCGCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCACTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTc                     <  1:65144/146‑1 (MQ=255)
                                                                                                                         gcgAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTcg                    <  7:245873/149‑1 (MQ=255)
                                                                                                                         gcgAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTcg                    >  7:50077/1‑149 (MQ=255)
                                                                                                                                gAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTgc             <  3:129690/149‑1 (MQ=255)
                                                                                                                                gAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGATGAAAGCGACCATCGTCAACCTGAAATCCTCTAAAGAAGAGCGTTTAGATGCAGTTGt                                                         >  5:252551/1‑105 (MQ=255)
                                                                                                                                      tCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGCCCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCGGCGCAAcc       >  7:189872/1‑149 (MQ=255)
                                                                                                                                         gCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCTTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAACCGtt    <  8:7637/149‑1 (MQ=255)
                                                                                                                                           tCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAACCGTTgc  >  2:80397/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
ACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCGTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGCAACCGTTGC  >  NC_002947/556856‑557143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑