Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 562,607 T→G 8.7% R111R (CGT→CGG)  rpsD → 30S ribosomal protein S4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947562,6070TG 8.7% 133.6 / 3.6 46R111R (CGT→CGG) rpsD30S ribosomal protein S4
Reads supporting (aligned to +/- strand):  ref base T (18/24);  new base G (2/2);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TCGTATCTACGGTGTTCTGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAAC  >  NC_002947/562460‑562755
                                                                                                                                                    |                                                                                                                                                       
tcgtATCTACGGTGTTCTGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGtt                                                                                                                                                        <  1:184607/149‑1 (MQ=255)
  gtATCTACGGTGTTCTGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTc                                                                                                                                                       <  7:46752/148‑1 (MQ=255)
     tCTACGGTGTTCTGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGa                                                                                                                                                    <  4:241711/148‑1 (MQ=255)
     tCTACGGTGTTCTGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGa                                                                                                                                                    <  2:17809/148‑1 (MQ=255)
       tACGGTGTTCTGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTc                                                                                                                                                 >  1:71437/1‑149 (MQ=255)
          ggTGTTCTGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACGTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGGAACGAGTcgag                                                                                                                                              <  4:120330/149‑4 (MQ=255)
            tgtTCTGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGtt                                                                                                                                                        >  1:253810/1‑137 (MQ=255)
            tgtTCTGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGtt                                                                                                                                                        <  2:253810/137‑1 (MQ=255)
             gtTCTGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTAACGGAATGGGGTGCGCAACGC‑AACGTTCCGGGTCCCGTCa                                                                                                                                           >  4:46300/1‑149 (MQ=255)
              ttCTGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAg                                                                                                                                          >  5:34433/1‑149 (MQ=255)
                 tGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTg                                                                                                                                       >  7:138288/1‑149 (MQ=255)
                   gAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGa                                                                                                                                                    <  1:181189/134‑1 (MQ=255)
                   gAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGa                                                                                                                                                    >  2:181189/1‑134 (MQ=255)
                   gAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCt                                                                                                                                        <  6:80276/146‑1 (MQ=255)
                       gTCAGTTCCGCGGTTACTAGCATGCTGCTGCCTCGAATAATGGCGCAAGCGGTGAGATCGTGCTGCAACTGCTCGAGTGCGGTGTGGTTAACGTCGTTTACCGTTTGTGCTTCGGCTCGA‑CTCGTTCCGAGTCCGGTCAGCTGGTTTCg                                                                                                                                 <  3:95373/149‑1 (MQ=255)
                       gTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTTGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCg                                                                                                                                 <  1:78449/149‑1 (MQ=255)
                          aGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑ca                                                                                                                            <  6:34433/148‑1 (MQ=255)
                             tCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGGAACGAGTcgag                                                                                                                                              >  4:229516/1‑127 (MQ=255)
                             tCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGGAACGAGTcgag                                                                                                                                              <  3:229516/130‑4 (MQ=255)
                                         acCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGt                                                                                                             >  4:173565/1‑148 (MQ=255)
                                           cAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGtt                                                                                                                                                        <  5:84286/106‑1 (MQ=255)
                                             aGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGa                                                                                                                                                    >  5:13685/1‑108 (MQ=255)
                                             aGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGa                                                                                                                                                    <  6:13685/108‑1 (MQ=255)
                                              gctgctTCCTGGAAAAAATGCGCAACCGGTTATTAACTTGTGGAACTGCTCGTGTGCCGTCTTGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACgg                                                                                                       <  3:46300/149‑1 (MQ=255)
                                              gctgctGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACgg                                                                                                       <  2:71437/149‑1 (MQ=255)
                                               ctgctgCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACgg                                                                                                       >  6:161563/1‑148 (MQ=255)
                                                     ccTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGa                                                                                                  <  6:198824/147‑1 (MQ=255)
                                                       tCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGGAACGAGTcgag                                                                                                                                              >  6:98525/1‑101 (MQ=255)
                                                          aaaaaaGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTaaa                                                                                           <  1:88948/149‑1 (MQ=255)
                                                           aaaaaGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCt                                                                                                                                        <  7:156806/106‑1 (MQ=255)
                                                           aaaaaGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCt                                                                                                                                        >  8:156806/1‑106 (MQ=255)
                                                               aGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTc                                                                                      >  4:111188/1‑149 (MQ=255)
                                                                   gcAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTcc                                                                                     <  4:222004/146‑1 (MQ=255)
                                                                   gcAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTcc                                                                                     >  3:222004/1‑146 (MQ=255)
                                                                          gTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCaa                                                                           <  5:164139/149‑1 (MQ=255)
                                                                                 ccTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTc                                                                    <  5:98974/149‑1 (MQ=255)
                                                                                     ctgcAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCgg                                                                 <  8:138288/148‑1 (MQ=255)
                                                                                        cAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTGCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGt                                                               <  5:122944/147‑1 (MQ=255)
                                                                                         aaCTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGAc                                                            <  6:270089/149‑1 (MQ=255)
                                                                                          aCTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACg                                                           <  5:139433/149‑1 (MQ=255)
                                                                                               tCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTc                                                      >  8:59754/1‑149 (MQ=255)
                                                                                                        gTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCGGGAGAAAAAGGGATCTGCTTCAAGTTTAAGAGTGAAATAATAACATACTACCAAGTTCGTGGGGGGGAGGTGGTCGAAGTc                                                >  1:219273/1‑149 (MQ=255)
                                                                                                        gTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCcgc                                             <  2:2942/149‑1 (MQ=255)
                                                                                                             gATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCgaga                                         >  6:82947/1‑148 (MQ=255)
                                                                                                                   gtcgtTTACCGTATGGGCTTCGGCTCGACCTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGc                                   >  1:153297/1‑149 (MQ=255)
                                                                                                                   gtcgtTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCt                                  >  5:254493/1‑149 (MQ=255)
                                                                                                                          aCCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCa                            <  3:173565/148‑1 (MQ=255)
                                                                                                                                   gCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCAtt                  >  1:144899/1‑149 (MQ=255)
                                                                                                                                           tCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCACAGTTCAAgg          >  2:66849/1‑148 (MQ=255)
                                                                                                                                             gA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGcc         <  5:161563/148‑1 (MQ=255)
                                                                                                                                                    ttCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAAc  >  1:190709/1‑149 (MQ=255)
                                                                                                                                                    ttCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAACCCTTGAAc  >  1:135702/1‑149 (MQ=255)
                                                                                                                                                    ttCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGCCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTgg                                <  4:200849/119‑1 (MQ=255)
                                                                                                                                                    ttCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGCCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTgg                                >  3:200849/1‑119 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
TCGTATCTACGGTGTTCTGGAGCGTCAGTTCCGCGGTTACTACCAAGCTGCTGCCTCGAAAAAAGGCGCAACCGGTGAGAACCTGCTGCAACTGCTCGAGTGCCGTCTGGATAACGTCGTTTACCGTATGGGCTTCGGCTCGA‑CTCGTTCCGAGTCCCGTCAGCTGGTTTCG‑‑‑CACAAAGCGATCAGCGTCAACGGTAAGACTGTAAACATTCCATCCTACCAAGTTCGTCCGGGTGACGTGGTCGCAGTCCGCGAGAAATCGCTGGGCCAGTTGCGCATTGTTCAAGCCCTTGAAC  >  NC_002947/562460‑562755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑