Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 566,625 T→A 7.7% intergenic (+37/+36) bfr‑I → / ← uvrA bacterioferritin/uvrABC excinuclease protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947566,6250TA 7.7% 177.4 / 3.6 61intergenic (+37/+36)bfr‑I/uvrAbacterioferritin/uvrABC excinuclease protein A
Reads supporting (aligned to +/- strand):  ref base T (25/31);  new base A (3/2);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

CTGCGTGCGCAGCTGGCCGACACCGAAGAAGATCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAGGCAATGATCTGCCCACCCTTGG  >  NC_002947/566478‑566770
                                                                                                                                                   |                                                                                                                                                 
cTGCGTGCGCAGCTGGCCGACACCGAAGAAGATCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCAt                                                                                                                                                   <  1:225067/148‑1 (MQ=255)
   cgtgcgCAGCTGGCCGACACCGAAGAAGATCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCgg                                                                                                                                                <  1:241171/148‑1 (MQ=255)
     tgcgCAGCTGGCCGACACCGAAGAAGATCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTAc                                                                                                                                             <  5:6048/149‑1 (MQ=255)
       cgcAGCTGGCCGACACCGAAGAAGATCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCa                                                                                                                                           >  4:166776/1‑149 (MQ=255)
          aGCTGGCCGACACCGAAGAAGATCACACCTACTGGCTCGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGTACCGATGGTGCCAggg                                                                                                                                        <  3:132299/149‑1 (MQ=255)
                       cGAAGAAGATCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCAtt                                                                                                                             >  3:40047/1‑147 (MQ=255)
                       cGAAGAAGATCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCAtt                                                                                                                             <  4:40047/147‑1 (MQ=255)
                       cGAAGAAGATCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTgc                                                                                                                           >  1:104204/1‑149 (MQ=255)
                       cGAAGAAGATCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTgc                                                                                                                           <  3:26479/149‑1 (MQ=255)
                         aagaagATCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTgcgc                                                                                                                         <  3:214804/149‑1 (MQ=255)
                          agaagaTCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTgcgc                                                                                                                         <  2:104204/148‑1 (MQ=255)
                            aagaTCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGGTGGAAAACTACTTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCgg                                                                                                                      >  1:70033/1‑149 (MQ=255)
                            aagaTCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCgg                                                                                                                      <  3:100607/149‑1 (MQ=255)
                              gaTCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGTACCGATGGTGCCAgg                                                                                                                                         <  5:85178/128‑1 (MQ=255)
                              gaTCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGTACCGATGGTGCCAgg                                                                                                                                         >  6:85178/1‑128 (MQ=255)
                                    acCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACt                                                                                                               >  5:29938/1‑148 (MQ=255)
                                    acCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACt                                                                                                               >  5:25941/1‑148 (MQ=255)
                                    acCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACt                                                                                                               >  7:141545/1‑148 (MQ=255)
                                    acCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATCTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTc                                                                                                              <  1:85960/149‑1 (MQ=255)
                                       tACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAg                                                                                                            >  7:30759/1‑148 (MQ=255)
                                         cTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAg                                                                                                            <  8:30759/146‑1 (MQ=255)
                                                  gaAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCttttt                                                                                                                                 <  3:205395/116‑1 (MQ=255)
                                                  gaAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCttttt                                                                                                                                 >  4:205395/1‑116 (MQ=255)
                                                      cagcagGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTcc                                                                                             <  6:32081/148‑1 (MQ=255)
                                                      cagcagGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTcc                                                                                             >  5:32081/1‑148 (MQ=255)
                                                      cagcagGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCa                                                                                            <  1:132825/149‑1 (MQ=255)
                                                         cagGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGc                                                                                          <  2:245827/148‑1 (MQ=255)
                                                               cTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTACAGCAATGGtt                                                                                   >  3:181827/1‑149 (MQ=255)
                                                                 gATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAgg                                                                                                           >  2:161032/1‑123 (MQ=255)
                                                                 gATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAgg                                                                                                           <  1:161032/123‑1 (MQ=255)
                                                                 gATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGttt                                                                                  >  4:103687/1‑148 (MQ=255)
                                                                   tCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTc                                                                                 <  8:23610/147‑1 (MQ=255)
                                                                   tCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTc                                                                                 >  7:23610/1‑147 (MQ=255)
                                                                        gCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAAt                                                                          <  4:167892/149‑1 (MQ=255)
                                                                        gCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAAt                                                                          <  8:141545/149‑1 (MQ=255)
                                                                                  tGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGtata                                                                >  4:202909/1‑149 (MQ=255)
                                                                                    gAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCAACGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGCTAATGGCCGGTatat                                                               >  6:37365/1‑148 (MQ=255)
                                                                                     aaaaCTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGa                                                             <  1:223361/149‑1 (MQ=255)
                                                                                     aaaaCTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGa                                                             <  3:166776/149‑1 (MQ=255)
                                                                                            ccTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTc                                                      <  8:134643/149‑1 (MQ=255)
                                                                                             cTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCa                                                     <  7:57093/149‑1 (MQ=255)
                                                                                              tGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCGCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCCGTATATGAATGCTTCa                                                     >  5:156945/1‑148 (MQ=255)
                                                                                                                  aTCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACt                                                           <  5:203273/122‑1 (MQ=255)
                                                                                                                  aTCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACt                                                           >  6:203273/1‑122 (MQ=255)
                                                                                                                      acaGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTc                                                                                 >  3:248393/1‑96 (MQ=255)
                                                                                                                      acaGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTc                                                                                 <  4:248393/96‑1 (MQ=255)
                                                                                                                            tCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTCCCGCAgg                       >  4:136891/1‑148 (MQ=255)
                                                                                                                            tCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAgg                       >  1:171681/1‑148 (MQ=255)
                                                                                                                              aaGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGTCTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAGGCaa                    >  5:235242/1‑149 (MQ=255)
                                                                                                                               aGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAGGCAAt                   <  5:37365/149‑1 (MQ=255)
                                                                                                                                   aaaGCCCCTGGCACCATCGGTACCATGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAGGCAATGAt                <  6:25941/148‑1 (MQ=255)
                                                                                                                                   aaaGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAGGCAATGAt                <  6:29938/148‑1 (MQ=255)
                                                                                                                                    aaGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCt                                              <  2:92826/117‑1 (MQ=255)
                                                                                                                                    aaGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCt                                              <  2:96285/117‑1 (MQ=255)
                                                                                                                                     aGCCCCTGGTACCGATGGTGCCAGGGGCTTTTACATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAGGCAATGATCTg             >  7:4017/1‑149 (MQ=255)
                                                                                                                                       ccccTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCAtctc                                                 >  5:226943/1‑111 (MQ=255)
                                                                                                                                       ccccTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCAtctc                                                 <  6:226943/111‑1 (MQ=255)
                                                                                                                                       ccccTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAGGCAATg                  >  7:195386/1‑142 (MQ=255)
                                                                                                                                       ccccTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAGGCAATg                  <  8:195386/142‑1 (MQ=255)
                                                                                                                                           tGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAGGCAATGATCTGCCCAcc       >  6:68225/1‑149 (MQ=255)
                                                                                                                                                ccATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAGGCAATGATCTGCCCACCCTTgg  <  2:70033/149‑1 (MQ=255)
                                                                                                                                                 cATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAGGCAATGATCTGCCCACCCTTgg  >  6:149697/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTGCGTGCGCAGCTGGCCGACACCGAAGAAGATCACACCTACTGGCTTGAGAAGCAGCAGGGCCTGATCAAGGCCATCGGGCTGGAAAACTACCTGCAGTCGCAGATGTAAGTTATCCACAGACTCAAGTAAAAGCCCCTGGCACCATCGGTACCAGGGGCTTTTTCATTGCGCCGGCAATACTCAGGCCCTATCCCGCTCCAGCAATGGTTTCAGGTAATGGCCGGTATATGACTGCTTCATCTCGCTCAGCTCTTCTGGTGTACCGCAGGCAATGATCTGCCCACCCTTGG  >  NC_002947/566478‑566770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑